Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0185 and Dshi_0186 overlap by 4 nucleotides Dshi_0186 and Dshi_0187 are separated by 22 nucleotides Dshi_0187 and Dshi_0188 are separated by 662 nucleotides
Dshi_0185: Dshi_0185 - phosphatidate cytidylyltransferase (RefSeq), at 186,744 to 187,694
_0185
Dshi_0186: Dshi_0186 - phospholipid/glycerol acyltransferase (RefSeq), at 187,691 to 188,344
_0186
Dshi_0187: Dshi_0187 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 188,367 to 188,981
_0187
Dshi_0188: Dshi_0188 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 189,644 to 190,600
_0188
Position (kb)
188
189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 187.543 kb on + strand, within Dshi_0185 at 187.774 kb on + strand at 187.806 kb on + strand, within Dshi_0186 at 187.814 kb on - strand, within Dshi_0186 at 188.180 kb on + strand, within Dshi_0186 at 188.411 kb on - strand at 188.618 kb on + strand, within Dshi_0187 at 188.818 kb on - strand, within Dshi_0187 at 188.911 kb on + strand, within Dshi_0187 at 188.918 kb on - strand, within Dshi_0187 at 189.014 kb on - strand at 189.044 kb on + strand at 189.052 kb on - strand at 189.075 kb on - strand at 189.145 kb on + strand at 189.152 kb on + strand at 189.160 kb on - strand at 189.169 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.179 kb on + strand at 189.218 kb on - strand at 189.413 kb on + strand at 189.554 kb on - strand at 189.570 kb on + strand at 189.578 kb on - strand at 189.578 kb on - strand at 189.588 kb on - strand at 189.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 187,543 + Dshi_0185 0.84 +0.5 187,774 + -0.6 187,806 + Dshi_0186 0.18 -0.7 187,814 - Dshi_0186 0.19 +0.4 188,180 + Dshi_0186 0.75 -0.6 188,411 - -1.3 188,618 + Dshi_0187 0.41 -0.6 188,818 - Dshi_0187 0.73 -0.4 188,911 + Dshi_0187 0.88 -1.8 188,918 - Dshi_0187 0.90 -0.7 189,014 - +0.1 189,044 + -0.6 189,052 - -1.0 189,075 - -0.3 189,145 + -1.0 189,152 + -0.2 189,160 - -0.3 189,169 - -1.3 189,172 - -0.5 189,172 - +0.4 189,172 - -0.8 189,179 + +0.5 189,218 - -0.2 189,413 + +2.0 189,554 - -0.2 189,570 + -0.6 189,578 - +2.6 189,578 - +0.3 189,588 - -2.2 189,618 - +1.0
Or see this region's nucleotide sequence