Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0027 and Dshi_0028 are separated by 7 nucleotides Dshi_0028 and Dshi_0029 are separated by 85 nucleotides Dshi_0029 and Dshi_0030 are separated by 11 nucleotides
Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807
_0027
Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909
_0028
Dshi_0029: Dshi_0029 - protein of unknown function DUF1159 (RefSeq), at 32,995 to 33,507
_0029
Dshi_0030: Dshi_0030 - thiol-disulfide oxidoreductase D, putative (RefSeq), at 33,519 to 34,190
_0030
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.821 kb on + strand, within Dshi_0027 at 30.836 kb on + strand, within Dshi_0027 at 30.837 kb on + strand, within Dshi_0027 at 30.857 kb on - strand, within Dshi_0027 at 30.920 kb on + strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 31.019 kb on - strand, within Dshi_0027 at 31.102 kb on - strand, within Dshi_0027 at 31.145 kb on + strand, within Dshi_0027 at 31.192 kb on + strand, within Dshi_0027 at 31.197 kb on + strand, within Dshi_0027 at 31.234 kb on + strand, within Dshi_0027 at 31.308 kb on + strand, within Dshi_0027 at 31.335 kb on + strand, within Dshi_0027 at 31.343 kb on - strand, within Dshi_0027 at 31.350 kb on + strand, within Dshi_0027 at 31.377 kb on - strand, within Dshi_0027 at 31.611 kb on + strand, within Dshi_0027 at 31.803 kb on + strand at 31.847 kb on - strand at 31.868 kb on - strand at 31.868 kb on - strand at 31.959 kb on + strand, within Dshi_0028 at 32.097 kb on + strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.249 kb on + strand, within Dshi_0028 at 32.376 kb on - strand, within Dshi_0028 at 32.520 kb on - strand, within Dshi_0028 at 32.629 kb on + strand, within Dshi_0028 at 32.678 kb on + strand, within Dshi_0028 at 32.949 kb on + strand at 32.956 kb on + strand at 32.961 kb on + strand at 33.586 kb on - strand at 33.601 kb on - strand, within Dshi_0030 at 33.717 kb on + strand, within Dshi_0030 at 33.759 kb on - strand, within Dshi_0030 at 33.853 kb on + strand, within Dshi_0030 at 33.854 kb on + strand, within Dshi_0030 at 33.863 kb on - strand, within Dshi_0030
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 30,821 + Dshi_0027 0.15 +0.2 30,836 + Dshi_0027 0.16 +0.2 30,837 + Dshi_0027 0.16 +1.6 30,857 - Dshi_0027 0.18 +0.5 30,920 + Dshi_0027 0.23 -1.6 30,982 - Dshi_0027 0.28 -0.4 30,982 - Dshi_0027 0.28 +0.6 31,019 - Dshi_0027 0.32 -0.6 31,102 - Dshi_0027 0.39 +1.0 31,145 + Dshi_0027 0.43 -0.4 31,192 + Dshi_0027 0.47 +1.0 31,197 + Dshi_0027 0.47 -0.0 31,234 + Dshi_0027 0.50 +1.0 31,308 + Dshi_0027 0.57 -0.6 31,335 + Dshi_0027 0.59 -0.0 31,343 - Dshi_0027 0.60 +1.0 31,350 + Dshi_0027 0.60 +0.2 31,377 - Dshi_0027 0.63 +1.2 31,611 + Dshi_0027 0.83 -0.9 31,803 + +0.3 31,847 - -0.8 31,868 - -0.8 31,868 - -0.9 31,959 + Dshi_0028 0.13 +0.6 32,097 + Dshi_0028 0.26 -0.7 32,166 - Dshi_0028 0.32 -0.3 32,166 - Dshi_0028 0.32 -1.6 32,249 + Dshi_0028 0.40 -0.0 32,376 - Dshi_0028 0.51 -1.2 32,520 - Dshi_0028 0.64 -2.4 32,629 + Dshi_0028 0.74 -1.0 32,678 + Dshi_0028 0.79 -0.6 32,949 + +0.5 32,956 + -1.8 32,961 + +0.6 33,586 - +0.2 33,601 - Dshi_0030 0.12 +0.4 33,717 + Dshi_0030 0.29 -0.2 33,759 - Dshi_0030 0.36 -0.6 33,853 + Dshi_0030 0.50 +1.0 33,854 + Dshi_0030 0.50 +0.8 33,863 - Dshi_0030 0.51 -0.8
Or see this region's nucleotide sequence