Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03686 and CCNA_03687 are separated by 73 nucleotides CCNA_03687 and CCNA_03688 are separated by 72 nucleotides CCNA_03688 and CCNA_03689 are separated by 119 nucleotides
CCNA_03686: CCNA_03686 - alpha2 macroglobulin domain-containing extracellular protein, at 3,848,258 to 3,853,318
_03686
CCNA_03687: CCNA_03687 - carbonic anhydrase, at 3,853,392 to 3,854,219
_03687
CCNA_03688: CCNA_03688 - AsnC-family transcriptional regulator, at 3,854,292 to 3,854,765
_03688
CCNA_03689: CCNA_03689 - alanine dehydrogenase, at 3,854,885 to 3,855,997
_03689
Position (kb) 3854
3855 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 3853.321 kb on + strand at 3853.321 kb on + strand at 3853.333 kb on + strand at 3853.333 kb on + strand at 3853.333 kb on + strand at 3853.333 kb on + strand at 3853.336 kb on - strand at 3853.336 kb on - strand at 3853.341 kb on - strand at 3853.341 kb on - strand at 3853.341 kb on - strand at 3853.341 kb on - strand at 3853.343 kb on + strand at 3853.344 kb on + strand at 3853.367 kb on + strand at 3853.367 kb on + strand at 3853.367 kb on + strand at 3853.375 kb on - strand at 3853.422 kb on - strand at 3853.424 kb on + strand at 3853.424 kb on + strand at 3853.424 kb on + strand at 3853.432 kb on - strand at 3853.432 kb on - strand at 3853.432 kb on - strand at 3853.432 kb on - strand at 3853.438 kb on + strand at 3853.456 kb on + strand at 3853.467 kb on - strand at 3853.467 kb on - strand at 3853.467 kb on - strand at 3853.492 kb on + strand, within CCNA_03687 at 3853.500 kb on - strand, within CCNA_03687 at 3853.593 kb on + strand, within CCNA_03687 at 3853.598 kb on + strand, within CCNA_03687 at 3853.606 kb on - strand, within CCNA_03687 at 3853.606 kb on - strand, within CCNA_03687 at 3853.615 kb on + strand, within CCNA_03687 at 3853.616 kb on - strand, within CCNA_03687 at 3853.616 kb on - strand, within CCNA_03687 at 3853.626 kb on - strand, within CCNA_03687 at 3853.628 kb on - strand, within CCNA_03687 at 3853.683 kb on + strand, within CCNA_03687 at 3853.683 kb on + strand, within CCNA_03687 at 3853.691 kb on - strand, within CCNA_03687 at 3853.695 kb on + strand, within CCNA_03687 at 3853.695 kb on + strand, within CCNA_03687 at 3853.703 kb on - strand, within CCNA_03687 at 3853.703 kb on - strand, within CCNA_03687 at 3853.705 kb on + strand, within CCNA_03687 at 3853.715 kb on - strand, within CCNA_03687 at 3853.745 kb on - strand, within CCNA_03687 at 3853.745 kb on - strand, within CCNA_03687 at 3853.750 kb on - strand, within CCNA_03687 at 3853.765 kb on - strand, within CCNA_03687 at 3853.803 kb on + strand, within CCNA_03687 at 3853.862 kb on + strand, within CCNA_03687 at 3853.870 kb on + strand, within CCNA_03687 at 3853.870 kb on + strand, within CCNA_03687 at 3853.870 kb on + strand, within CCNA_03687 at 3853.870 kb on - strand, within CCNA_03687 at 3853.882 kb on + strand, within CCNA_03687 at 3853.905 kb on - strand, within CCNA_03687 at 3853.906 kb on - strand, within CCNA_03687 at 3853.915 kb on - strand, within CCNA_03687 at 3853.915 kb on - strand, within CCNA_03687 at 3853.926 kb on - strand, within CCNA_03687 at 3853.935 kb on + strand, within CCNA_03687 at 3853.944 kb on + strand, within CCNA_03687 at 3853.957 kb on - strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.959 kb on + strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.967 kb on - strand, within CCNA_03687 at 3853.994 kb on + strand, within CCNA_03687 at 3854.001 kb on + strand, within CCNA_03687 at 3854.001 kb on + strand, within CCNA_03687 at 3854.001 kb on + strand, within CCNA_03687 at 3854.009 kb on - strand, within CCNA_03687 at 3854.009 kb on - strand, within CCNA_03687 at 3854.034 kb on - strand, within CCNA_03687 at 3854.046 kb on + strand, within CCNA_03687 at 3854.046 kb on + strand, within CCNA_03687 at 3854.054 kb on - strand, within CCNA_03687 at 3854.070 kb on - strand, within CCNA_03687 at 3854.137 kb on - strand at 3854.150 kb on - strand at 3854.168 kb on - strand at 3854.253 kb on + strand at 3854.253 kb on + strand at 3854.253 kb on + strand at 3854.268 kb on + strand at 3854.276 kb on - strand at 3854.286 kb on - strand at 3854.319 kb on - strand at 3854.319 kb on - strand at 3854.325 kb on + strand at 3854.325 kb on + strand at 3854.333 kb on - strand at 3854.333 kb on - strand at 3854.354 kb on - strand, within CCNA_03688 at 3854.354 kb on - strand, within CCNA_03688 at 3854.354 kb on - strand, within CCNA_03688 at 3854.354 kb on - strand, within CCNA_03688 at 3854.355 kb on - strand, within CCNA_03688 at 3854.356 kb on + strand, within CCNA_03688 at 3854.367 kb on - strand, within CCNA_03688 at 3854.468 kb on - strand, within CCNA_03688 at 3854.468 kb on - strand, within CCNA_03688 at 3854.469 kb on - strand, within CCNA_03688 at 3854.485 kb on + strand, within CCNA_03688 at 3854.488 kb on - strand, within CCNA_03688 at 3854.493 kb on - strand, within CCNA_03688 at 3854.523 kb on - strand, within CCNA_03688 at 3854.564 kb on + strand, within CCNA_03688 at 3854.564 kb on + strand, within CCNA_03688 at 3854.588 kb on + strand, within CCNA_03688 at 3854.621 kb on - strand, within CCNA_03688 at 3854.658 kb on - strand, within CCNA_03688 at 3854.678 kb on + strand, within CCNA_03688 at 3854.806 kb on + strand at 3854.934 kb on + strand at 3854.934 kb on + strand at 3854.934 kb on + strand at 3854.942 kb on - strand at 3854.942 kb on - strand at 3854.942 kb on - strand at 3854.971 kb on + strand at 3854.974 kb on - strand at 3855.029 kb on + strand, within CCNA_03689 at 3855.033 kb on + strand, within CCNA_03689 at 3855.036 kb on - strand, within CCNA_03689 at 3855.074 kb on + strand, within CCNA_03689 at 3855.074 kb on + strand, within CCNA_03689 at 3855.103 kb on + strand, within CCNA_03689 at 3855.103 kb on + strand, within CCNA_03689 at 3855.103 kb on + strand, within CCNA_03689 at 3855.103 kb on + strand, within CCNA_03689 at 3855.111 kb on - strand, within CCNA_03689 at 3855.118 kb on - strand, within CCNA_03689 at 3855.155 kb on + strand at 3855.155 kb on + strand, within CCNA_03689 at 3855.163 kb on - strand, within CCNA_03689 at 3855.205 kb on + strand, within CCNA_03689 at 3855.205 kb on + strand, within CCNA_03689 at 3855.205 kb on + strand, within CCNA_03689 at 3855.224 kb on + strand, within CCNA_03689 at 3855.305 kb on + strand, within CCNA_03689 at 3855.328 kb on - strand, within CCNA_03689 at 3855.364 kb on - strand, within CCNA_03689 at 3855.369 kb on - strand, within CCNA_03689 at 3855.383 kb on + strand, within CCNA_03689 at 3855.391 kb on - strand, within CCNA_03689 at 3855.409 kb on - strand, within CCNA_03689 at 3855.480 kb on + strand, within CCNA_03689 at 3855.518 kb on - strand, within CCNA_03689 at 3855.545 kb on + strand, within CCNA_03689 at 3855.571 kb on + strand, within CCNA_03689 at 3855.571 kb on + strand, within CCNA_03689 at 3855.571 kb on + strand, within CCNA_03689 at 3855.579 kb on - strand, within CCNA_03689 at 3855.599 kb on + strand, within CCNA_03689 at 3855.599 kb on + strand, within CCNA_03689 at 3855.634 kb on - strand, within CCNA_03689 at 3855.646 kb on + strand at 3855.646 kb on + strand, within CCNA_03689 at 3855.646 kb on + strand, within CCNA_03689 at 3855.654 kb on - strand, within CCNA_03689 at 3855.654 kb on - strand, within CCNA_03689 at 3855.663 kb on - strand, within CCNA_03689 at 3855.666 kb on + strand, within CCNA_03689 at 3855.700 kb on - strand, within CCNA_03689 at 3855.705 kb on + strand, within CCNA_03689 at 3855.705 kb on + strand, within CCNA_03689 at 3855.705 kb on + strand, within CCNA_03689 at 3855.729 kb on + strand, within CCNA_03689 at 3855.737 kb on + strand, within CCNA_03689 at 3855.737 kb on - strand, within CCNA_03689 at 3855.743 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689 at 3855.764 kb on + strand, within CCNA_03689
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 3,853,321 + +0.1 3,853,321 + +0.8 3,853,333 + -1.4 3,853,333 + +0.1 3,853,333 + -1.0 3,853,333 + +0.8 3,853,336 - +0.2 3,853,336 - +0.8 3,853,341 - +0.5 3,853,341 - -0.2 3,853,341 - +0.5 3,853,341 - +1.7 3,853,343 + +0.1 3,853,344 + -1.2 3,853,367 + -0.2 3,853,367 + -2.0 3,853,367 + -0.4 3,853,375 - +1.9 3,853,422 - -0.6 3,853,424 + +0.1 3,853,424 + +0.7 3,853,424 + -0.1 3,853,432 - -0.7 3,853,432 - +0.2 3,853,432 - +1.4 3,853,432 - -0.2 3,853,438 + -2.4 3,853,456 + -0.4 3,853,467 - -0.2 3,853,467 - -0.7 3,853,467 - -1.5 3,853,492 + CCNA_03687 0.12 -1.3 3,853,500 - CCNA_03687 0.13 +0.6 3,853,593 + CCNA_03687 0.24 +0.1 3,853,598 + CCNA_03687 0.25 +0.4 3,853,606 - CCNA_03687 0.26 -1.0 3,853,606 - CCNA_03687 0.26 -1.6 3,853,615 + CCNA_03687 0.27 +1.4 3,853,616 - CCNA_03687 0.27 +0.7 3,853,616 - CCNA_03687 0.27 -0.7 3,853,626 - CCNA_03687 0.28 +0.4 3,853,628 - CCNA_03687 0.29 -1.7 3,853,683 + CCNA_03687 0.35 -1.6 3,853,683 + CCNA_03687 0.35 -0.9 3,853,691 - CCNA_03687 0.36 +0.3 3,853,695 + CCNA_03687 0.37 +2.4 3,853,695 + CCNA_03687 0.37 -0.4 3,853,703 - CCNA_03687 0.38 +0.7 3,853,703 - CCNA_03687 0.38 -2.1 3,853,705 + CCNA_03687 0.38 -0.7 3,853,715 - CCNA_03687 0.39 -1.2 3,853,745 - CCNA_03687 0.43 +0.5 3,853,745 - CCNA_03687 0.43 -0.2 3,853,750 - CCNA_03687 0.43 -0.7 3,853,765 - CCNA_03687 0.45 +0.9 3,853,803 + CCNA_03687 0.50 +0.8 3,853,862 + CCNA_03687 0.57 -0.2 3,853,870 + CCNA_03687 0.58 -1.7 3,853,870 + CCNA_03687 0.58 -1.7 3,853,870 + CCNA_03687 0.58 +0.8 3,853,870 - CCNA_03687 0.58 -0.7 3,853,882 + CCNA_03687 0.59 -1.2 3,853,905 - CCNA_03687 0.62 -0.1 3,853,906 - CCNA_03687 0.62 -0.2 3,853,915 - CCNA_03687 0.63 -1.4 3,853,915 - CCNA_03687 0.63 -0.9 3,853,926 - CCNA_03687 0.64 +1.4 3,853,935 + CCNA_03687 0.66 -0.4 3,853,944 + CCNA_03687 0.67 -1.7 3,853,957 - CCNA_03687 0.68 +1.8 3,853,959 + CCNA_03687 0.68 +0.4 3,853,959 + CCNA_03687 0.68 -2.3 3,853,959 + CCNA_03687 0.68 -2.0 3,853,959 + CCNA_03687 0.68 -0.4 3,853,959 + CCNA_03687 0.68 +1.4 3,853,959 + CCNA_03687 0.68 -1.9 3,853,959 + CCNA_03687 0.68 -1.5 3,853,959 + CCNA_03687 0.68 -1.0 3,853,967 - CCNA_03687 0.69 +1.6 3,853,967 - CCNA_03687 0.69 +0.8 3,853,967 - CCNA_03687 0.69 -0.2 3,853,967 - CCNA_03687 0.69 -1.5 3,853,967 - CCNA_03687 0.69 -0.9 3,853,967 - CCNA_03687 0.69 -1.0 3,853,967 - CCNA_03687 0.69 +0.6 3,853,967 - CCNA_03687 0.69 +1.4 3,853,994 + CCNA_03687 0.73 +0.3 3,854,001 + CCNA_03687 0.74 +0.1 3,854,001 + CCNA_03687 0.74 -0.6 3,854,001 + CCNA_03687 0.74 -2.0 3,854,009 - CCNA_03687 0.75 +0.2 3,854,009 - CCNA_03687 0.75 -1.2 3,854,034 - CCNA_03687 0.78 +3.7 3,854,046 + CCNA_03687 0.79 +0.9 3,854,046 + CCNA_03687 0.79 -1.1 3,854,054 - CCNA_03687 0.80 -0.5 3,854,070 - CCNA_03687 0.82 +1.4 3,854,137 - -0.7 3,854,150 - -2.4 3,854,168 - -0.8 3,854,253 + +0.8 3,854,253 + -0.9 3,854,253 + +0.1 3,854,268 + -0.8 3,854,276 - +0.6 3,854,286 - +4.4 3,854,319 - +1.8 3,854,319 - +1.8 3,854,325 + +1.4 3,854,325 + -0.4 3,854,333 - +2.1 3,854,333 - +2.1 3,854,354 - CCNA_03688 0.13 +1.7 3,854,354 - CCNA_03688 0.13 +1.7 3,854,354 - CCNA_03688 0.13 +1.7 3,854,354 - CCNA_03688 0.13 +2.3 3,854,355 - CCNA_03688 0.13 +1.9 3,854,356 + CCNA_03688 0.14 +2.4 3,854,367 - CCNA_03688 0.16 +3.1 3,854,468 - CCNA_03688 0.37 +2.3 3,854,468 - CCNA_03688 0.37 +2.0 3,854,469 - CCNA_03688 0.37 +1.3 3,854,485 + CCNA_03688 0.41 +2.4 3,854,488 - CCNA_03688 0.41 +1.8 3,854,493 - CCNA_03688 0.42 +1.5 3,854,523 - CCNA_03688 0.49 +1.1 3,854,564 + CCNA_03688 0.57 -0.8 3,854,564 + CCNA_03688 0.57 +0.8 3,854,588 + CCNA_03688 0.62 +0.4 3,854,621 - CCNA_03688 0.69 +1.8 3,854,658 - CCNA_03688 0.77 +2.0 3,854,678 + CCNA_03688 0.81 +1.4 3,854,806 + +4.0 3,854,934 + +1.3 3,854,934 + +2.4 3,854,934 + +1.9 3,854,942 - +0.1 3,854,942 - +0.4 3,854,942 - -0.4 3,854,971 + +1.4 3,854,974 - +0.4 3,855,029 + CCNA_03689 0.13 +2.5 3,855,033 + CCNA_03689 0.13 +2.4 3,855,036 - CCNA_03689 0.14 -0.3 3,855,074 + CCNA_03689 0.17 +2.8 3,855,074 + CCNA_03689 0.17 +2.6 3,855,103 + CCNA_03689 0.20 +3.1 3,855,103 + CCNA_03689 0.20 +2.7 3,855,103 + CCNA_03689 0.20 +2.6 3,855,103 + CCNA_03689 0.20 +2.8 3,855,111 - CCNA_03689 0.20 -1.2 3,855,118 - CCNA_03689 0.21 -1.2 3,855,155 + +2.4 3,855,155 + CCNA_03689 0.24 +2.7 3,855,163 - CCNA_03689 0.25 +1.4 3,855,205 + CCNA_03689 0.29 +2.8 3,855,205 + CCNA_03689 0.29 +2.9 3,855,205 + CCNA_03689 0.29 +3.0 3,855,224 + CCNA_03689 0.30 +3.7 3,855,305 + CCNA_03689 0.38 +4.2 3,855,328 - CCNA_03689 0.40 -3.3 3,855,364 - CCNA_03689 0.43 +0.8 3,855,369 - CCNA_03689 0.43 -0.2 3,855,383 + CCNA_03689 0.45 +3.5 3,855,391 - CCNA_03689 0.45 +0.4 3,855,409 - CCNA_03689 0.47 +1.4 3,855,480 + CCNA_03689 0.53 +2.0 3,855,518 - CCNA_03689 0.57 +2.4 3,855,545 + CCNA_03689 0.59 +3.1 3,855,571 + CCNA_03689 0.62 +3.0 3,855,571 + CCNA_03689 0.62 +3.0 3,855,571 + CCNA_03689 0.62 +2.7 3,855,579 - CCNA_03689 0.62 -0.2 3,855,599 + CCNA_03689 0.64 +3.8 3,855,599 + CCNA_03689 0.64 +2.8 3,855,634 - CCNA_03689 0.67 +0.4 3,855,646 + +2.4 3,855,646 + CCNA_03689 0.68 +1.9 3,855,646 + CCNA_03689 0.68 +2.7 3,855,654 - CCNA_03689 0.69 +0.8 3,855,654 - CCNA_03689 0.69 -0.8 3,855,663 - CCNA_03689 0.70 +0.1 3,855,666 + CCNA_03689 0.70 +3.1 3,855,700 - CCNA_03689 0.73 -0.6 3,855,705 + CCNA_03689 0.74 +2.7 3,855,705 + CCNA_03689 0.74 +3.3 3,855,705 + CCNA_03689 0.74 +2.9 3,855,729 + CCNA_03689 0.76 +3.9 3,855,737 + CCNA_03689 0.77 +3.5 3,855,737 - CCNA_03689 0.77 -1.2 3,855,743 + CCNA_03689 0.77 +3.4 3,855,764 + CCNA_03689 0.79 +3.7 3,855,764 + CCNA_03689 0.79 +3.3 3,855,764 + CCNA_03689 0.79 +4.1 3,855,764 + CCNA_03689 0.79 +3.4 3,855,764 + CCNA_03689 0.79 +3.7 3,855,764 + CCNA_03689 0.79 +3.2 3,855,764 + CCNA_03689 0.79 +3.6 3,855,764 + CCNA_03689 0.79 +3.7 3,855,764 + CCNA_03689 0.79 +4.1 3,855,764 + CCNA_03689 0.79 +3.6 3,855,764 + CCNA_03689 0.79 +3.9 3,855,764 + CCNA_03689 0.79 +3.6 3,855,764 + CCNA_03689 0.79 +3.7
Or see this region's nucleotide sequence