Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02373 and CCNA_02374 overlap by 7 nucleotides CCNA_02374 and CCNA_02375 are separated by 58 nucleotides CCNA_02375 and CCNA_02376 overlap by 12 nucleotides CCNA_02376 and CCNA_02377 overlap by 4 nucleotides CCNA_02377 and CCNA_R0055 are separated by 225 nucleotides
CCNA_02373: CCNA_02373 - transposase, at 2,518,748 to 2,519,674
_02373
CCNA_02374: CCNA_02374 - transposase, at 2,519,668 to 2,519,934
_02374
CCNA_02375: CCNA_02375 - hypothetical protein, at 2,519,993 to 2,520,256
_02375
CCNA_02376: CCNA_02376 - hypothetical protein, at 2,520,245 to 2,520,523
_02376
CCNA_02377: CCNA_02377 - hypothetical protein, at 2,520,520 to 2,521,242
_02377
CCNA_R0055: CCNA_R0055 - tRNA-Ile, at 2,521,468 to 2,521,543
_R0055
Position (kb)
2520
2521 Strain fitness (log2 ratio)
-1
0
1
2 at 2519.999 kb on + strand at 2519.999 kb on + strand at 2520.068 kb on + strand, within CCNA_02375 at 2520.076 kb on - strand, within CCNA_02375 at 2520.076 kb on - strand, within CCNA_02375 at 2520.092 kb on + strand, within CCNA_02375 at 2520.109 kb on - strand, within CCNA_02375 at 2520.137 kb on + strand, within CCNA_02375 at 2520.137 kb on + strand, within CCNA_02375 at 2520.182 kb on - strand, within CCNA_02375 at 2520.184 kb on - strand, within CCNA_02375 at 2520.209 kb on - strand, within CCNA_02375 at 2520.221 kb on - strand, within CCNA_02375 at 2520.223 kb on + strand, within CCNA_02375 at 2520.251 kb on + strand at 2520.251 kb on + strand at 2520.259 kb on - strand at 2520.393 kb on + strand, within CCNA_02376 at 2520.414 kb on + strand, within CCNA_02376 at 2520.481 kb on - strand, within CCNA_02376 at 2520.491 kb on - strand, within CCNA_02376 at 2520.525 kb on - strand at 2520.549 kb on + strand at 2520.583 kb on - strand at 2520.595 kb on + strand, within CCNA_02377 at 2520.602 kb on - strand, within CCNA_02377 at 2520.603 kb on - strand, within CCNA_02377 at 2520.603 kb on - strand, within CCNA_02377 at 2520.615 kb on + strand, within CCNA_02377 at 2520.659 kb on - strand, within CCNA_02377 at 2520.727 kb on + strand, within CCNA_02377 at 2520.735 kb on - strand, within CCNA_02377 at 2520.741 kb on - strand, within CCNA_02377 at 2520.760 kb on - strand, within CCNA_02377 at 2520.820 kb on + strand, within CCNA_02377 at 2520.871 kb on - strand, within CCNA_02377 at 2520.893 kb on + strand, within CCNA_02377 at 2520.955 kb on - strand, within CCNA_02377 at 2520.982 kb on + strand, within CCNA_02377 at 2521.002 kb on - strand, within CCNA_02377 at 2521.002 kb on - strand, within CCNA_02377 at 2521.003 kb on - strand, within CCNA_02377 at 2521.027 kb on + strand, within CCNA_02377 at 2521.034 kb on - strand, within CCNA_02377 at 2521.034 kb on - strand, within CCNA_02377 at 2521.081 kb on + strand, within CCNA_02377 at 2521.081 kb on + strand, within CCNA_02377 at 2521.089 kb on - strand, within CCNA_02377 at 2521.089 kb on - strand, within CCNA_02377 at 2521.089 kb on - strand, within CCNA_02377 at 2521.089 kb on - strand, within CCNA_02377 at 2521.094 kb on - strand, within CCNA_02377 at 2521.104 kb on + strand, within CCNA_02377 at 2521.236 kb on - strand at 2521.264 kb on + strand at 2521.272 kb on - strand at 2521.369 kb on - strand at 2521.369 kb on - strand at 2521.385 kb on + strand at 2521.393 kb on - strand at 2521.393 kb on - strand at 2521.409 kb on - strand at 2521.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,519,999 + +0.7 2,519,999 + +0.6 2,520,068 + CCNA_02375 0.28 -1.4 2,520,076 - CCNA_02375 0.31 +0.1 2,520,076 - CCNA_02375 0.31 -0.6 2,520,092 + CCNA_02375 0.38 -1.7 2,520,109 - CCNA_02375 0.44 +1.7 2,520,137 + CCNA_02375 0.55 -0.9 2,520,137 + CCNA_02375 0.55 +0.9 2,520,182 - CCNA_02375 0.72 +0.4 2,520,184 - CCNA_02375 0.72 -1.0 2,520,209 - CCNA_02375 0.82 +2.3 2,520,221 - CCNA_02375 0.86 +1.7 2,520,223 + CCNA_02375 0.87 -0.7 2,520,251 + -0.7 2,520,251 + -1.4 2,520,259 - -1.4 2,520,393 + CCNA_02376 0.53 -0.7 2,520,414 + CCNA_02376 0.61 +1.1 2,520,481 - CCNA_02376 0.85 -0.7 2,520,491 - CCNA_02376 0.88 -0.4 2,520,525 - +0.7 2,520,549 + -0.7 2,520,583 - -0.5 2,520,595 + CCNA_02377 0.10 +0.7 2,520,602 - CCNA_02377 0.11 -0.4 2,520,603 - CCNA_02377 0.11 +0.4 2,520,603 - CCNA_02377 0.11 +1.7 2,520,615 + CCNA_02377 0.13 +1.1 2,520,659 - CCNA_02377 0.19 -0.6 2,520,727 + CCNA_02377 0.29 -0.7 2,520,735 - CCNA_02377 0.30 +0.3 2,520,741 - CCNA_02377 0.31 +2.1 2,520,760 - CCNA_02377 0.33 +0.1 2,520,820 + CCNA_02377 0.41 +0.1 2,520,871 - CCNA_02377 0.49 -1.6 2,520,893 + CCNA_02377 0.52 +1.1 2,520,955 - CCNA_02377 0.60 +1.7 2,520,982 + CCNA_02377 0.64 +1.1 2,521,002 - CCNA_02377 0.67 +0.4 2,521,002 - CCNA_02377 0.67 -0.7 2,521,003 - CCNA_02377 0.67 -0.1 2,521,027 + CCNA_02377 0.70 +0.7 2,521,034 - CCNA_02377 0.71 -0.3 2,521,034 - CCNA_02377 0.71 +0.4 2,521,081 + CCNA_02377 0.78 -0.4 2,521,081 + CCNA_02377 0.78 +0.4 2,521,089 - CCNA_02377 0.79 -0.1 2,521,089 - CCNA_02377 0.79 +0.4 2,521,089 - CCNA_02377 0.79 +0.4 2,521,089 - CCNA_02377 0.79 -0.4 2,521,094 - CCNA_02377 0.79 -1.1 2,521,104 + CCNA_02377 0.81 +1.1 2,521,236 - +1.1 2,521,264 + -0.3 2,521,272 - +0.7 2,521,369 - +2.7 2,521,369 - +1.1 2,521,385 + +1.1 2,521,393 - +0.7 2,521,393 - -0.1 2,521,409 - +0.7 2,521,409 - -0.3
Or see this region's nucleotide sequence