Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02316 and CCNA_02317 overlap by 91 nucleotides CCNA_02317 and CCNA_02318 are separated by 28 nucleotides CCNA_02318 and CCNA_02319 are separated by 116 nucleotides CCNA_02319 and CCNA_02320 are separated by 118 nucleotides
CCNA_02316: CCNA_02316 - hypothetical protein, at 2,460,427 to 2,460,942
_02316
CCNA_02317: CCNA_02317 - hypothetical protein, at 2,460,852 to 2,461,289
_02317
CCNA_02318: CCNA_02318 - hypothetical protein, at 2,461,318 to 2,461,581
_02318
CCNA_02319: CCNA_02319 - hypothetical protein, at 2,461,698 to 2,462,216
_02319
CCNA_02320: CCNA_02320 - 2'-deoxycytidine 5'-triphosphate deaminase, at 2,462,335 to 2,463,360
_02320
Position (kb)
2461
2462
2463 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2460.713 kb on + strand, within CCNA_02316 at 2460.721 kb on - strand, within CCNA_02316 at 2460.730 kb on + strand, within CCNA_02316 at 2460.740 kb on - strand, within CCNA_02316 at 2460.743 kb on - strand, within CCNA_02316 at 2460.743 kb on - strand, within CCNA_02316 at 2460.743 kb on - strand, within CCNA_02316 at 2460.764 kb on - strand, within CCNA_02316 at 2460.771 kb on - strand, within CCNA_02316 at 2460.825 kb on - strand, within CCNA_02316 at 2460.825 kb on - strand, within CCNA_02316 at 2460.855 kb on + strand at 2460.872 kb on + strand at 2460.877 kb on + strand at 2460.885 kb on - strand at 2460.895 kb on + strand at 2460.900 kb on + strand at 2460.908 kb on - strand at 2460.949 kb on + strand, within CCNA_02317 at 2460.949 kb on + strand, within CCNA_02317 at 2460.957 kb on - strand, within CCNA_02317 at 2460.957 kb on - strand, within CCNA_02317 at 2460.994 kb on + strand, within CCNA_02317 at 2461.025 kb on + strand, within CCNA_02317 at 2461.025 kb on + strand, within CCNA_02317 at 2461.033 kb on - strand, within CCNA_02317 at 2461.036 kb on + strand, within CCNA_02317 at 2461.036 kb on + strand, within CCNA_02317 at 2461.036 kb on + strand, within CCNA_02317 at 2461.044 kb on - strand, within CCNA_02317 at 2461.044 kb on - strand, within CCNA_02317 at 2461.061 kb on - strand, within CCNA_02317 at 2461.130 kb on - strand, within CCNA_02317 at 2461.130 kb on - strand, within CCNA_02317 at 2461.247 kb on - strand at 2461.247 kb on - strand at 2461.263 kb on + strand at 2461.310 kb on - strand at 2461.326 kb on - strand at 2461.360 kb on + strand, within CCNA_02318 at 2461.379 kb on - strand, within CCNA_02318 at 2461.379 kb on - strand, within CCNA_02318 at 2461.426 kb on - strand, within CCNA_02318 at 2461.440 kb on + strand, within CCNA_02318 at 2461.440 kb on + strand, within CCNA_02318 at 2461.441 kb on + strand, within CCNA_02318 at 2461.444 kb on - strand, within CCNA_02318 at 2461.449 kb on - strand, within CCNA_02318 at 2461.451 kb on + strand, within CCNA_02318 at 2461.479 kb on - strand, within CCNA_02318 at 2461.489 kb on + strand, within CCNA_02318 at 2461.790 kb on - strand, within CCNA_02319 at 2461.805 kb on - strand, within CCNA_02319 at 2461.822 kb on + strand, within CCNA_02319 at 2461.822 kb on + strand, within CCNA_02319 at 2461.836 kb on + strand, within CCNA_02319 at 2461.837 kb on - strand, within CCNA_02319 at 2461.844 kb on - strand, within CCNA_02319 at 2461.857 kb on + strand, within CCNA_02319 at 2461.865 kb on - strand, within CCNA_02319 at 2461.870 kb on - strand, within CCNA_02319 at 2461.903 kb on - strand, within CCNA_02319 at 2461.987 kb on - strand, within CCNA_02319 at 2462.010 kb on + strand, within CCNA_02319 at 2462.012 kb on + strand, within CCNA_02319 at 2462.020 kb on - strand, within CCNA_02319 at 2462.039 kb on + strand, within CCNA_02319 at 2462.044 kb on + strand, within CCNA_02319 at 2462.052 kb on - strand, within CCNA_02319 at 2462.052 kb on - strand, within CCNA_02319 at 2462.052 kb on - strand, within CCNA_02319 at 2462.062 kb on + strand, within CCNA_02319 at 2462.098 kb on + strand, within CCNA_02319 at 2462.250 kb on + strand at 2462.289 kb on - strand at 2462.318 kb on + strand at 2462.331 kb on - strand at 2462.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,460,713 + CCNA_02316 0.55 -0.5 2,460,721 - CCNA_02316 0.57 +3.8 2,460,730 + CCNA_02316 0.59 +1.5 2,460,740 - CCNA_02316 0.61 +0.9 2,460,743 - CCNA_02316 0.61 +2.4 2,460,743 - CCNA_02316 0.61 +0.7 2,460,743 - CCNA_02316 0.61 +1.8 2,460,764 - CCNA_02316 0.65 +1.8 2,460,771 - CCNA_02316 0.67 +0.1 2,460,825 - CCNA_02316 0.77 +1.9 2,460,825 - CCNA_02316 0.77 +1.0 2,460,855 + +2.5 2,460,872 + -0.9 2,460,877 + +1.4 2,460,885 - -1.0 2,460,895 + +1.6 2,460,900 + +1.0 2,460,908 - +0.2 2,460,949 + CCNA_02317 0.22 +1.6 2,460,949 + CCNA_02317 0.22 +0.2 2,460,957 - CCNA_02317 0.24 -0.6 2,460,957 - CCNA_02317 0.24 -0.0 2,460,994 + CCNA_02317 0.32 -1.0 2,461,025 + CCNA_02317 0.39 -0.4 2,461,025 + CCNA_02317 0.39 +1.0 2,461,033 - CCNA_02317 0.41 -1.5 2,461,036 + CCNA_02317 0.42 +1.6 2,461,036 + CCNA_02317 0.42 +2.6 2,461,036 + CCNA_02317 0.42 +2.6 2,461,044 - CCNA_02317 0.44 -1.4 2,461,044 - CCNA_02317 0.44 -2.0 2,461,061 - CCNA_02317 0.48 +0.2 2,461,130 - CCNA_02317 0.63 +1.0 2,461,130 - CCNA_02317 0.63 -2.2 2,461,247 - +0.8 2,461,247 - +0.7 2,461,263 + -0.0 2,461,310 - +0.5 2,461,326 - -3.7 2,461,360 + CCNA_02318 0.16 -1.0 2,461,379 - CCNA_02318 0.23 -1.0 2,461,379 - CCNA_02318 0.23 +2.6 2,461,426 - CCNA_02318 0.41 -0.2 2,461,440 + CCNA_02318 0.46 -0.0 2,461,440 + CCNA_02318 0.46 -0.4 2,461,441 + CCNA_02318 0.47 +2.7 2,461,444 - CCNA_02318 0.48 +0.5 2,461,449 - CCNA_02318 0.50 -2.2 2,461,451 + CCNA_02318 0.50 -0.2 2,461,479 - CCNA_02318 0.61 +1.0 2,461,489 + CCNA_02318 0.65 +0.7 2,461,790 - CCNA_02319 0.18 -1.3 2,461,805 - CCNA_02319 0.21 -1.0 2,461,822 + CCNA_02319 0.24 -1.0 2,461,822 + CCNA_02319 0.24 +1.6 2,461,836 + CCNA_02319 0.27 -0.2 2,461,837 - CCNA_02319 0.27 -1.1 2,461,844 - CCNA_02319 0.28 -1.3 2,461,857 + CCNA_02319 0.31 -1.1 2,461,865 - CCNA_02319 0.32 -1.3 2,461,870 - CCNA_02319 0.33 -0.8 2,461,903 - CCNA_02319 0.39 +2.6 2,461,987 - CCNA_02319 0.56 -1.0 2,462,010 + CCNA_02319 0.60 +0.2 2,462,012 + CCNA_02319 0.61 -0.6 2,462,020 - CCNA_02319 0.62 +0.8 2,462,039 + CCNA_02319 0.66 -0.0 2,462,044 + CCNA_02319 0.67 -0.2 2,462,052 - CCNA_02319 0.68 -2.1 2,462,052 - CCNA_02319 0.68 -1.3 2,462,052 - CCNA_02319 0.68 +1.6 2,462,062 + CCNA_02319 0.70 +0.2 2,462,098 + CCNA_02319 0.77 -1.8 2,462,250 + -1.0 2,462,289 - +1.8 2,462,318 + -1.1 2,462,331 - +0.4 2,462,353 - +1.4
Or see this region's nucleotide sequence