Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01961 and CCNA_01962 are separated by 99 nucleotides CCNA_01962 and CCNA_01963 overlap by 64 nucleotides CCNA_01963 and CCNA_01964 are separated by 4 nucleotides
CCNA_01961: CCNA_01961 - biotin carboxylase, at 2,104,868 to 2,106,214
_01961
CCNA_01962: CCNA_01962 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,106,314 to 2,106,967
_01962
CCNA_01963: CCNA_01963 - hypothetical protein, at 2,106,904 to 2,107,542
_01963
CCNA_01964: CCNA_01964 - NADH dehydrogenase, at 2,107,547 to 2,107,942
_01964
Position (kb)
2106
2107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.288 kb on + strand at 2106.298 kb on + strand at 2106.332 kb on + strand at 2106.341 kb on - strand at 2106.363 kb on + strand at 2106.422 kb on - strand, within CCNA_01962 at 2106.466 kb on - strand, within CCNA_01962 at 2106.484 kb on + strand, within CCNA_01962 at 2106.515 kb on + strand, within CCNA_01962 at 2106.554 kb on - strand, within CCNA_01962 at 2106.603 kb on - strand at 2106.615 kb on - strand, within CCNA_01962 at 2106.703 kb on - strand, within CCNA_01962 at 2106.723 kb on - strand, within CCNA_01962 at 2106.723 kb on - strand, within CCNA_01962 at 2106.783 kb on + strand, within CCNA_01962 at 2106.783 kb on + strand, within CCNA_01962 at 2106.813 kb on - strand, within CCNA_01962 at 2106.874 kb on + strand, within CCNA_01962 at 2106.874 kb on + strand, within CCNA_01962 at 2106.882 kb on - strand, within CCNA_01962 at 2106.947 kb on - strand at 2106.970 kb on - strand, within CCNA_01963 at 2106.976 kb on + strand, within CCNA_01963 at 2107.009 kb on + strand, within CCNA_01963 at 2107.009 kb on + strand, within CCNA_01963 at 2107.009 kb on + strand, within CCNA_01963 at 2107.017 kb on - strand, within CCNA_01963 at 2107.027 kb on - strand, within CCNA_01963 at 2107.061 kb on + strand, within CCNA_01963 at 2107.061 kb on + strand, within CCNA_01963 at 2107.061 kb on + strand, within CCNA_01963 at 2107.069 kb on - strand, within CCNA_01963 at 2107.069 kb on - strand, within CCNA_01963 at 2107.165 kb on - strand, within CCNA_01963 at 2107.165 kb on - strand, within CCNA_01963 at 2107.214 kb on + strand, within CCNA_01963 at 2107.222 kb on - strand, within CCNA_01963 at 2107.354 kb on - strand, within CCNA_01963 at 2107.360 kb on + strand, within CCNA_01963 at 2107.506 kb on - strand at 2107.552 kb on - strand at 2107.612 kb on - strand, within CCNA_01964 at 2107.631 kb on - strand, within CCNA_01964
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 2,106,288 + +1.7 2,106,288 + +0.9 2,106,288 + +1.8 2,106,288 + +1.9 2,106,298 + +2.9 2,106,332 + +2.0 2,106,341 - +1.1 2,106,363 + +2.9 2,106,422 - CCNA_01962 0.17 -0.9 2,106,466 - CCNA_01962 0.23 -1.6 2,106,484 + CCNA_01962 0.26 +1.7 2,106,515 + CCNA_01962 0.31 +0.2 2,106,554 - CCNA_01962 0.37 +0.6 2,106,603 - +0.1 2,106,615 - CCNA_01962 0.46 -1.1 2,106,703 - CCNA_01962 0.59 -1.3 2,106,723 - CCNA_01962 0.63 -0.2 2,106,723 - CCNA_01962 0.63 -1.6 2,106,783 + CCNA_01962 0.72 -1.0 2,106,783 + CCNA_01962 0.72 +0.5 2,106,813 - CCNA_01962 0.76 -0.7 2,106,874 + CCNA_01962 0.86 -0.1 2,106,874 + CCNA_01962 0.86 -0.8 2,106,882 - CCNA_01962 0.87 -1.3 2,106,947 - -0.9 2,106,970 - CCNA_01963 0.10 +1.1 2,106,976 + CCNA_01963 0.11 -0.3 2,107,009 + CCNA_01963 0.16 -0.5 2,107,009 + CCNA_01963 0.16 -0.3 2,107,009 + CCNA_01963 0.16 -1.6 2,107,017 - CCNA_01963 0.18 +1.4 2,107,027 - CCNA_01963 0.19 -0.5 2,107,061 + CCNA_01963 0.25 -1.3 2,107,061 + CCNA_01963 0.25 +0.4 2,107,061 + CCNA_01963 0.25 -0.1 2,107,069 - CCNA_01963 0.26 +0.3 2,107,069 - CCNA_01963 0.26 +0.9 2,107,165 - CCNA_01963 0.41 +1.2 2,107,165 - CCNA_01963 0.41 +1.0 2,107,214 + CCNA_01963 0.49 -0.5 2,107,222 - CCNA_01963 0.50 +1.7 2,107,354 - CCNA_01963 0.70 +1.4 2,107,360 + CCNA_01963 0.71 -1.8 2,107,506 - +2.1 2,107,552 - +2.2 2,107,612 - CCNA_01964 0.16 +2.3 2,107,631 - CCNA_01964 0.21 +2.1
Or see this region's nucleotide sequence