Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00546 and CCNA_00547 are separated by 294 nucleotides CCNA_00547 and CCNA_00548 are separated by 24 nucleotides CCNA_00548 and CCNA_00549 overlap by 7 nucleotides CCNA_00549 and CCNA_00550 overlap by 85 nucleotides
CCNA_00546: CCNA_00546 - hypothetical protein, at 564,671 to 565,453
_00546
CCNA_00547: CCNA_00547 - PurR-family transcriptional regulator, at 565,748 to 565,879
_00547
CCNA_00548: CCNA_00548 - transposase, HTH-Tnp1 superfamily, at 565,904 to 566,170
_00548
CCNA_00549: CCNA_00549 - transposase, IS2 family, at 566,164 to 567,090
_00549
CCNA_00550: CCNA_00550 - LacI-family transcriptional regulator, at 567,006 to 568,052
_00550
Position (kb)
566
567
568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 565.602 kb on + strand at 565.620 kb on - strand at 565.662 kb on - strand at 565.663 kb on + strand at 565.671 kb on - strand at 565.753 kb on + strand at 565.753 kb on + strand at 565.753 kb on + strand at 565.753 kb on + strand at 565.753 kb on + strand at 565.761 kb on - strand at 565.782 kb on - strand, within CCNA_00547 at 565.810 kb on + strand, within CCNA_00547 at 565.810 kb on + strand, within CCNA_00547 at 565.810 kb on + strand, within CCNA_00547 at 565.810 kb on + strand, within CCNA_00547 at 565.818 kb on - strand, within CCNA_00547 at 565.847 kb on - strand, within CCNA_00547 at 565.881 kb on - strand at 565.881 kb on - strand at 565.881 kb on - strand at 565.881 kb on - strand at 567.093 kb on + strand at 567.093 kb on + strand at 567.130 kb on - strand, within CCNA_00550 at 567.142 kb on - strand, within CCNA_00550 at 567.184 kb on + strand, within CCNA_00550 at 567.184 kb on + strand, within CCNA_00550 at 567.189 kb on + strand at 567.192 kb on - strand, within CCNA_00550 at 567.192 kb on - strand, within CCNA_00550 at 567.192 kb on - strand, within CCNA_00550 at 567.219 kb on - strand, within CCNA_00550 at 567.219 kb on - strand, within CCNA_00550 at 567.219 kb on - strand, within CCNA_00550 at 567.221 kb on + strand, within CCNA_00550 at 567.229 kb on - strand, within CCNA_00550 at 567.229 kb on - strand, within CCNA_00550 at 567.253 kb on - strand, within CCNA_00550 at 567.260 kb on - strand, within CCNA_00550 at 567.278 kb on - strand, within CCNA_00550 at 567.364 kb on - strand, within CCNA_00550 at 567.373 kb on - strand, within CCNA_00550 at 567.385 kb on + strand, within CCNA_00550 at 567.385 kb on + strand, within CCNA_00550 at 567.398 kb on + strand, within CCNA_00550 at 567.407 kb on - strand, within CCNA_00550 at 567.407 kb on - strand, within CCNA_00550 at 567.425 kb on + strand, within CCNA_00550 at 567.435 kb on + strand, within CCNA_00550 at 567.448 kb on + strand, within CCNA_00550 at 567.466 kb on - strand, within CCNA_00550 at 567.466 kb on - strand, within CCNA_00550 at 567.471 kb on + strand, within CCNA_00550 at 567.471 kb on + strand, within CCNA_00550 at 567.478 kb on + strand, within CCNA_00550 at 567.479 kb on - strand, within CCNA_00550 at 567.486 kb on - strand, within CCNA_00550 at 567.486 kb on - strand, within CCNA_00550 at 567.521 kb on + strand, within CCNA_00550 at 567.524 kb on - strand, within CCNA_00550 at 567.524 kb on - strand, within CCNA_00550 at 567.529 kb on - strand, within CCNA_00550 at 567.541 kb on + strand, within CCNA_00550 at 567.595 kb on - strand, within CCNA_00550 at 567.596 kb on + strand, within CCNA_00550 at 567.647 kb on + strand, within CCNA_00550 at 567.655 kb on - strand, within CCNA_00550 at 567.660 kb on + strand, within CCNA_00550 at 567.660 kb on + strand, within CCNA_00550 at 567.660 kb on + strand, within CCNA_00550 at 567.667 kb on + strand, within CCNA_00550 at 567.668 kb on - strand, within CCNA_00550 at 567.675 kb on - strand, within CCNA_00550 at 567.675 kb on - strand, within CCNA_00550 at 567.716 kb on + strand, within CCNA_00550 at 567.786 kb on + strand, within CCNA_00550 at 567.786 kb on + strand, within CCNA_00550 at 567.794 kb on - strand, within CCNA_00550 at 567.804 kb on + strand, within CCNA_00550 at 567.828 kb on + strand at 567.841 kb on - strand, within CCNA_00550 at 567.842 kb on + strand, within CCNA_00550 at 567.862 kb on + strand, within CCNA_00550 at 567.870 kb on - strand, within CCNA_00550 at 567.886 kb on + strand, within CCNA_00550 at 567.894 kb on - strand, within CCNA_00550 at 567.905 kb on - strand, within CCNA_00550 at 567.925 kb on - strand, within CCNA_00550 at 567.936 kb on + strand, within CCNA_00550 at 567.944 kb on - strand, within CCNA_00550 at 567.991 kb on - strand at 568.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 565,602 + +0.2 565,620 - +0.1 565,662 - +0.3 565,663 + -0.4 565,671 - -0.8 565,753 + -0.0 565,753 + +0.5 565,753 + +0.4 565,753 + +1.5 565,753 + -0.5 565,761 - +0.2 565,782 - CCNA_00547 0.26 -2.8 565,810 + CCNA_00547 0.47 +0.9 565,810 + CCNA_00547 0.47 +2.4 565,810 + CCNA_00547 0.47 +0.1 565,810 + CCNA_00547 0.47 -0.4 565,818 - CCNA_00547 0.53 -0.9 565,847 - CCNA_00547 0.75 -1.5 565,881 - +2.7 565,881 - +2.6 565,881 - +3.5 565,881 - +2.0 567,093 + -0.5 567,093 + +0.1 567,130 - CCNA_00550 0.12 +0.5 567,142 - CCNA_00550 0.13 +0.9 567,184 + CCNA_00550 0.17 -0.5 567,184 + CCNA_00550 0.17 +0.5 567,189 + +2.9 567,192 - CCNA_00550 0.18 +3.9 567,192 - CCNA_00550 0.18 +4.3 567,192 - CCNA_00550 0.18 +4.5 567,219 - CCNA_00550 0.20 +0.6 567,219 - CCNA_00550 0.20 +1.7 567,219 - CCNA_00550 0.20 +1.1 567,221 + CCNA_00550 0.21 +1.9 567,229 - CCNA_00550 0.21 -0.3 567,229 - CCNA_00550 0.21 -1.7 567,253 - CCNA_00550 0.24 -0.1 567,260 - CCNA_00550 0.24 +2.3 567,278 - CCNA_00550 0.26 -0.9 567,364 - CCNA_00550 0.34 +0.4 567,373 - CCNA_00550 0.35 +2.9 567,385 + CCNA_00550 0.36 -0.4 567,385 + CCNA_00550 0.36 -0.5 567,398 + CCNA_00550 0.37 -1.7 567,407 - CCNA_00550 0.38 -0.8 567,407 - CCNA_00550 0.38 -0.2 567,425 + CCNA_00550 0.40 -1.5 567,435 + CCNA_00550 0.41 -0.3 567,448 + CCNA_00550 0.42 +0.6 567,466 - CCNA_00550 0.44 -0.9 567,466 - CCNA_00550 0.44 -0.1 567,471 + CCNA_00550 0.44 +0.9 567,471 + CCNA_00550 0.44 +0.1 567,478 + CCNA_00550 0.45 +0.3 567,479 - CCNA_00550 0.45 -1.3 567,486 - CCNA_00550 0.46 -0.5 567,486 - CCNA_00550 0.46 -0.7 567,521 + CCNA_00550 0.49 -0.2 567,524 - CCNA_00550 0.49 -2.0 567,524 - CCNA_00550 0.49 -0.8 567,529 - CCNA_00550 0.50 +0.9 567,541 + CCNA_00550 0.51 -1.5 567,595 - CCNA_00550 0.56 +2.9 567,596 + CCNA_00550 0.56 +2.5 567,647 + CCNA_00550 0.61 +1.9 567,655 - CCNA_00550 0.62 +1.9 567,660 + CCNA_00550 0.62 +0.9 567,660 + CCNA_00550 0.62 +1.3 567,660 + CCNA_00550 0.62 -0.1 567,667 + CCNA_00550 0.63 +0.8 567,668 - CCNA_00550 0.63 -0.8 567,675 - CCNA_00550 0.64 -1.5 567,675 - CCNA_00550 0.64 +0.3 567,716 + CCNA_00550 0.68 -0.5 567,786 + CCNA_00550 0.74 -0.0 567,786 + CCNA_00550 0.74 +0.3 567,794 - CCNA_00550 0.75 -0.5 567,804 + CCNA_00550 0.76 +1.1 567,828 + +2.9 567,841 - CCNA_00550 0.80 -0.8 567,842 + CCNA_00550 0.80 +0.4 567,862 + CCNA_00550 0.82 -0.6 567,870 - CCNA_00550 0.83 +0.3 567,886 + CCNA_00550 0.84 -1.2 567,894 - CCNA_00550 0.85 +1.9 567,905 - CCNA_00550 0.86 +0.5 567,925 - CCNA_00550 0.88 +1.0 567,936 + CCNA_00550 0.89 -1.7 567,944 - CCNA_00550 0.90 -0.9 567,991 - +0.1 568,008 + -1.3
Or see this region's nucleotide sequence