Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00514 and CCNA_00515 are separated by 415 nucleotides CCNA_00515 and CCNA_00516 are separated by 25 nucleotides CCNA_00516 and CCNA_00517 are separated by 2 nucleotides
CCNA_00514: CCNA_00514 - pirin, at 528,078 to 528,908
_00514
CCNA_00515: CCNA_00515 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 529,324 to 531,657
_00515
CCNA_00516: CCNA_00516 - integral membrane protein, at 531,683 to 532,432
_00516
CCNA_00517: CCNA_00517 - peptidyl-tRNA hydrolase, at 532,435 to 533,052
_00517
Position (kb) 529
530
531
532 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 528.330 kb on + strand, within CCNA_00514 at 528.337 kb on - strand, within CCNA_00514 at 528.338 kb on - strand, within CCNA_00514 at 528.369 kb on + strand, within CCNA_00514 at 528.405 kb on + strand, within CCNA_00514 at 528.405 kb on + strand, within CCNA_00514 at 528.413 kb on - strand, within CCNA_00514 at 528.424 kb on + strand, within CCNA_00514 at 528.424 kb on + strand, within CCNA_00514 at 528.432 kb on - strand, within CCNA_00514 at 528.432 kb on - strand, within CCNA_00514 at 528.436 kb on + strand, within CCNA_00514 at 528.436 kb on + strand, within CCNA_00514 at 528.621 kb on + strand, within CCNA_00514 at 528.645 kb on - strand, within CCNA_00514 at 528.645 kb on - strand, within CCNA_00514 at 528.662 kb on + strand, within CCNA_00514 at 528.690 kb on + strand, within CCNA_00514 at 528.690 kb on + strand, within CCNA_00514 at 528.698 kb on - strand, within CCNA_00514 at 528.713 kb on - strand, within CCNA_00514 at 528.757 kb on - strand, within CCNA_00514 at 528.777 kb on + strand, within CCNA_00514 at 528.802 kb on - strand, within CCNA_00514 at 528.832 kb on + strand at 528.856 kb on + strand at 528.864 kb on + strand at 528.864 kb on - strand at 528.878 kb on - strand at 528.902 kb on + strand at 528.910 kb on - strand at 528.915 kb on - strand at 528.972 kb on + strand at 529.040 kb on - strand at 529.074 kb on - strand at 529.127 kb on + strand at 529.147 kb on + strand at 529.147 kb on + strand at 529.152 kb on + strand at 529.155 kb on - strand at 529.155 kb on - strand at 529.155 kb on - strand at 529.160 kb on - strand at 529.189 kb on - strand at 529.264 kb on + strand at 529.371 kb on - strand at 529.398 kb on + strand at 529.405 kb on + strand at 529.423 kb on + strand at 529.429 kb on - strand at 529.439 kb on - strand at 529.449 kb on - strand at 529.450 kb on - strand at 529.527 kb on - strand at 529.539 kb on - strand at 529.544 kb on - strand at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.570 kb on + strand, within CCNA_00515 at 529.578 kb on - strand, within CCNA_00515 at 529.630 kb on + strand, within CCNA_00515 at 529.638 kb on - strand, within CCNA_00515 at 529.638 kb on - strand, within CCNA_00515 at 529.660 kb on + strand, within CCNA_00515 at 529.674 kb on - strand, within CCNA_00515 at 529.810 kb on + strand, within CCNA_00515 at 529.849 kb on - strand, within CCNA_00515 at 529.869 kb on + strand, within CCNA_00515 at 529.878 kb on + strand, within CCNA_00515 at 529.878 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.884 kb on + strand, within CCNA_00515 at 529.886 kb on - strand, within CCNA_00515 at 529.892 kb on - strand, within CCNA_00515 at 529.892 kb on - strand, within CCNA_00515 at 529.898 kb on - strand, within CCNA_00515 at 530.014 kb on + strand, within CCNA_00515 at 530.021 kb on - strand, within CCNA_00515 at 530.038 kb on + strand, within CCNA_00515 at 530.054 kb on + strand, within CCNA_00515 at 530.056 kb on - strand, within CCNA_00515 at 530.059 kb on + strand, within CCNA_00515 at 530.059 kb on + strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.067 kb on - strand, within CCNA_00515 at 530.147 kb on - strand, within CCNA_00515 at 530.159 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.165 kb on + strand, within CCNA_00515 at 530.167 kb on - strand, within CCNA_00515 at 530.170 kb on + strand, within CCNA_00515 at 530.173 kb on - strand, within CCNA_00515 at 530.173 kb on - strand, within CCNA_00515 at 530.175 kb on + strand, within CCNA_00515 at 530.241 kb on + strand, within CCNA_00515 at 530.296 kb on + strand, within CCNA_00515 at 530.298 kb on + strand, within CCNA_00515 at 530.298 kb on + strand, within CCNA_00515 at 530.377 kb on + strand, within CCNA_00515 at 530.378 kb on + strand, within CCNA_00515 at 530.434 kb on + strand, within CCNA_00515 at 530.442 kb on + strand, within CCNA_00515 at 530.442 kb on - strand, within CCNA_00515 at 530.480 kb on - strand, within CCNA_00515 at 530.588 kb on - strand, within CCNA_00515 at 530.629 kb on - strand, within CCNA_00515 at 530.652 kb on - strand, within CCNA_00515 at 530.661 kb on + strand, within CCNA_00515 at 530.681 kb on - strand, within CCNA_00515 at 530.742 kb on + strand, within CCNA_00515 at 530.743 kb on + strand, within CCNA_00515 at 530.748 kb on + strand, within CCNA_00515 at 530.748 kb on + strand, within CCNA_00515 at 530.751 kb on - strand, within CCNA_00515 at 530.808 kb on + strand, within CCNA_00515 at 530.810 kb on - strand, within CCNA_00515 at 530.822 kb on + strand, within CCNA_00515 at 530.822 kb on + strand, within CCNA_00515 at 530.866 kb on + strand, within CCNA_00515 at 530.866 kb on + strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.874 kb on - strand, within CCNA_00515 at 530.879 kb on - strand, within CCNA_00515 at 530.900 kb on + strand, within CCNA_00515 at 530.915 kb on + strand, within CCNA_00515 at 530.923 kb on - strand, within CCNA_00515 at 530.925 kb on + strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.933 kb on - strand, within CCNA_00515 at 530.940 kb on - strand, within CCNA_00515 at 530.969 kb on - strand, within CCNA_00515 at 531.011 kb on + strand, within CCNA_00515 at 531.018 kb on + strand, within CCNA_00515 at 531.019 kb on - strand, within CCNA_00515 at 531.027 kb on - strand, within CCNA_00515 at 531.036 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.067 kb on + strand, within CCNA_00515 at 531.127 kb on + strand, within CCNA_00515 at 531.135 kb on - strand, within CCNA_00515 at 531.234 kb on + strand, within CCNA_00515 at 531.244 kb on + strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.252 kb on - strand, within CCNA_00515 at 531.256 kb on + strand, within CCNA_00515 at 531.259 kb on + strand, within CCNA_00515 at 531.304 kb on - strand, within CCNA_00515 at 531.316 kb on + strand, within CCNA_00515 at 531.403 kb on + strand, within CCNA_00515 at 531.407 kb on - strand, within CCNA_00515 at 531.442 kb on - strand at 531.445 kb on + strand at 531.445 kb on + strand at 531.453 kb on - strand at 531.462 kb on - strand at 531.463 kb on - strand at 531.471 kb on + strand at 531.512 kb on - strand at 531.514 kb on - strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.552 kb on + strand at 531.560 kb on - strand at 531.563 kb on + strand at 531.582 kb on - strand at 531.584 kb on + strand at 531.660 kb on - strand at 531.682 kb on + strand at 531.717 kb on + strand at 531.725 kb on - strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.747 kb on + strand at 531.755 kb on - strand at 531.755 kb on - strand at 531.755 kb on - strand at 531.825 kb on + strand, within CCNA_00516 at 531.825 kb on + strand, within CCNA_00516 at 531.833 kb on + strand, within CCNA_00516 at 531.850 kb on - strand, within CCNA_00516 at 531.870 kb on - strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.872 kb on + strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.880 kb on - strand, within CCNA_00516 at 531.882 kb on + strand, within CCNA_00516 at 531.887 kb on + strand, within CCNA_00516 at 531.890 kb on - strand, within CCNA_00516 at 531.890 kb on - strand, within CCNA_00516 at 531.895 kb on - strand, within CCNA_00516 at 531.907 kb on + strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.915 kb on - strand, within CCNA_00516 at 531.939 kb on + strand, within CCNA_00516 at 531.939 kb on + strand, within CCNA_00516 at 531.947 kb on - strand, within CCNA_00516 at 531.947 kb on - strand, within CCNA_00516 at 531.960 kb on + strand, within CCNA_00516 at 531.967 kb on - strand, within CCNA_00516 at 532.014 kb on + strand, within CCNA_00516 at 532.019 kb on + strand, within CCNA_00516 at 532.024 kb on + strand, within CCNA_00516 at 532.037 kb on + strand, within CCNA_00516 at 532.070 kb on - strand, within CCNA_00516 at 532.070 kb on - strand, within CCNA_00516 at 532.087 kb on - strand, within CCNA_00516 at 532.109 kb on - strand, within CCNA_00516 at 532.113 kb on - strand, within CCNA_00516 at 532.177 kb on - strand, within CCNA_00516 at 532.249 kb on - strand, within CCNA_00516 at 532.266 kb on + strand, within CCNA_00516 at 532.272 kb on + strand, within CCNA_00516 at 532.275 kb on - strand, within CCNA_00516 at 532.284 kb on - strand, within CCNA_00516 at 532.298 kb on - strand, within CCNA_00516 at 532.334 kb on + strand, within CCNA_00516 at 532.337 kb on - strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.355 kb on + strand, within CCNA_00516 at 532.363 kb on - strand at 532.383 kb on + strand at 532.403 kb on + strand at 532.461 kb on + strand at 532.461 kb on + strand at 532.461 kb on + strand at 532.474 kb on - strand at 532.501 kb on - strand, within CCNA_00517 at 532.501 kb on - strand, within CCNA_00517 at 532.501 kb on - strand, within CCNA_00517
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 528,330 + CCNA_00514 0.30 +1.2 528,337 - CCNA_00514 0.31 -0.3 528,338 - CCNA_00514 0.31 +1.2 528,369 + CCNA_00514 0.35 -0.5 528,405 + CCNA_00514 0.39 -2.2 528,405 + CCNA_00514 0.39 +0.9 528,413 - CCNA_00514 0.40 -0.0 528,424 + CCNA_00514 0.42 +0.7 528,424 + CCNA_00514 0.42 -0.2 528,432 - CCNA_00514 0.43 -0.0 528,432 - CCNA_00514 0.43 -2.5 528,436 + CCNA_00514 0.43 -0.0 528,436 + CCNA_00514 0.43 -0.8 528,621 + CCNA_00514 0.65 -2.4 528,645 - CCNA_00514 0.68 -1.8 528,645 - CCNA_00514 0.68 -1.0 528,662 + CCNA_00514 0.70 +0.6 528,690 + CCNA_00514 0.74 +1.8 528,690 + CCNA_00514 0.74 -1.2 528,698 - CCNA_00514 0.75 +0.5 528,713 - CCNA_00514 0.76 +0.1 528,757 - CCNA_00514 0.82 -1.4 528,777 + CCNA_00514 0.84 +1.0 528,802 - CCNA_00514 0.87 -0.5 528,832 + +0.4 528,856 + +3.4 528,864 + -0.4 528,864 - +1.2 528,878 - +1.8 528,902 + -0.4 528,910 - +1.1 528,915 - -0.2 528,972 + +0.6 529,040 - -0.2 529,074 - +2.2 529,127 + -1.2 529,147 + +0.8 529,147 + +0.9 529,152 + +0.5 529,155 - -1.8 529,155 - +2.8 529,155 - -0.8 529,160 - +0.2 529,189 - -1.3 529,264 + +0.2 529,371 - +0.2 529,398 + +0.9 529,405 + +1.1 529,423 + +0.8 529,429 - +0.3 529,439 - +0.2 529,449 - +0.4 529,450 - -0.0 529,527 - -0.8 529,539 - -2.2 529,544 - -0.5 529,570 + CCNA_00515 0.11 +1.7 529,570 + CCNA_00515 0.11 +0.9 529,570 + CCNA_00515 0.11 -0.0 529,570 + CCNA_00515 0.11 +1.8 529,578 - CCNA_00515 0.11 -1.1 529,630 + CCNA_00515 0.13 +0.4 529,638 - CCNA_00515 0.13 -0.4 529,638 - CCNA_00515 0.13 +1.8 529,660 + CCNA_00515 0.14 -0.0 529,674 - CCNA_00515 0.15 -0.2 529,810 + CCNA_00515 0.21 -0.5 529,849 - CCNA_00515 0.22 -0.2 529,869 + CCNA_00515 0.23 +0.2 529,878 + CCNA_00515 0.24 +1.2 529,878 + CCNA_00515 0.24 -2.3 529,884 + CCNA_00515 0.24 +0.4 529,884 + CCNA_00515 0.24 -0.5 529,884 + CCNA_00515 0.24 -0.2 529,884 + CCNA_00515 0.24 +0.8 529,886 - CCNA_00515 0.24 -0.9 529,892 - CCNA_00515 0.24 +0.5 529,892 - CCNA_00515 0.24 -1.1 529,898 - CCNA_00515 0.25 -1.9 530,014 + CCNA_00515 0.30 +1.0 530,021 - CCNA_00515 0.30 -1.6 530,038 + CCNA_00515 0.31 +0.6 530,054 + CCNA_00515 0.31 +0.2 530,056 - CCNA_00515 0.31 +0.5 530,059 + CCNA_00515 0.31 +0.4 530,059 + CCNA_00515 0.31 +0.5 530,067 - CCNA_00515 0.32 -0.1 530,067 - CCNA_00515 0.32 -1.2 530,067 - CCNA_00515 0.32 +0.7 530,067 - CCNA_00515 0.32 -0.4 530,067 - CCNA_00515 0.32 +0.5 530,067 - CCNA_00515 0.32 +1.6 530,067 - CCNA_00515 0.32 -0.8 530,147 - CCNA_00515 0.35 +2.5 530,159 + CCNA_00515 0.36 +0.6 530,165 + CCNA_00515 0.36 -0.7 530,165 + CCNA_00515 0.36 -0.3 530,165 + CCNA_00515 0.36 -0.1 530,167 - CCNA_00515 0.36 -1.2 530,170 + CCNA_00515 0.36 +2.1 530,173 - CCNA_00515 0.36 -0.7 530,173 - CCNA_00515 0.36 -0.4 530,175 + CCNA_00515 0.36 +1.0 530,241 + CCNA_00515 0.39 +1.7 530,296 + CCNA_00515 0.42 +1.4 530,298 + CCNA_00515 0.42 +1.6 530,298 + CCNA_00515 0.42 +3.5 530,377 + CCNA_00515 0.45 +1.4 530,378 + CCNA_00515 0.45 +2.2 530,434 + CCNA_00515 0.48 +1.1 530,442 + CCNA_00515 0.48 +0.2 530,442 - CCNA_00515 0.48 +0.5 530,480 - CCNA_00515 0.50 -1.4 530,588 - CCNA_00515 0.54 -1.1 530,629 - CCNA_00515 0.56 +1.2 530,652 - CCNA_00515 0.57 +0.5 530,661 + CCNA_00515 0.57 +0.3 530,681 - CCNA_00515 0.58 +0.7 530,742 + CCNA_00515 0.61 +1.6 530,743 + CCNA_00515 0.61 -0.1 530,748 + CCNA_00515 0.61 +2.5 530,748 + CCNA_00515 0.61 +0.4 530,751 - CCNA_00515 0.61 -0.9 530,808 + CCNA_00515 0.64 +0.8 530,810 - CCNA_00515 0.64 -0.7 530,822 + CCNA_00515 0.64 -0.7 530,822 + CCNA_00515 0.64 +0.0 530,866 + CCNA_00515 0.66 -0.3 530,866 + CCNA_00515 0.66 +1.5 530,874 - CCNA_00515 0.66 -1.3 530,874 - CCNA_00515 0.66 -1.0 530,874 - CCNA_00515 0.66 +0.8 530,874 - CCNA_00515 0.66 -1.2 530,879 - CCNA_00515 0.67 +2.2 530,900 + CCNA_00515 0.68 +0.6 530,915 + CCNA_00515 0.68 -1.8 530,923 - CCNA_00515 0.69 +1.3 530,925 + CCNA_00515 0.69 +1.6 530,933 - CCNA_00515 0.69 +0.1 530,933 - CCNA_00515 0.69 +1.8 530,933 - CCNA_00515 0.69 +0.5 530,940 - CCNA_00515 0.69 +0.1 530,969 - CCNA_00515 0.70 -0.2 531,011 + CCNA_00515 0.72 +2.8 531,018 + CCNA_00515 0.73 -0.7 531,019 - CCNA_00515 0.73 +1.2 531,027 - CCNA_00515 0.73 -0.5 531,036 + CCNA_00515 0.73 -0.1 531,067 + CCNA_00515 0.75 +1.8 531,067 + CCNA_00515 0.75 +1.5 531,067 + CCNA_00515 0.75 +1.9 531,067 + CCNA_00515 0.75 +0.9 531,127 + CCNA_00515 0.77 +0.5 531,135 - CCNA_00515 0.78 +2.0 531,234 + CCNA_00515 0.82 -0.4 531,244 + CCNA_00515 0.82 +0.2 531,252 - CCNA_00515 0.83 +1.8 531,252 - CCNA_00515 0.83 +0.8 531,252 - CCNA_00515 0.83 +1.2 531,252 - CCNA_00515 0.83 +0.8 531,252 - CCNA_00515 0.83 -0.4 531,252 - CCNA_00515 0.83 +0.8 531,256 + CCNA_00515 0.83 +1.6 531,259 + CCNA_00515 0.83 +0.7 531,304 - CCNA_00515 0.85 -1.2 531,316 + CCNA_00515 0.85 +1.5 531,403 + CCNA_00515 0.89 +1.6 531,407 - CCNA_00515 0.89 +1.6 531,442 - -0.7 531,445 + +1.7 531,445 + +0.6 531,453 - +1.0 531,462 - +0.4 531,463 - +0.8 531,471 + +1.0 531,512 - +0.8 531,514 - -0.2 531,552 + +2.1 531,552 + +1.8 531,552 + +3.8 531,552 + +2.2 531,560 - +0.6 531,563 + +2.1 531,582 - +0.3 531,584 + +3.0 531,660 - +2.8 531,682 + -0.3 531,717 + +2.8 531,725 - -1.4 531,746 + -0.0 531,746 + +0.6 531,747 + +1.6 531,755 - +1.5 531,755 - -1.1 531,755 - +0.1 531,825 + CCNA_00516 0.19 -0.4 531,825 + CCNA_00516 0.19 +0.1 531,833 + CCNA_00516 0.20 -0.9 531,850 - CCNA_00516 0.22 +0.8 531,870 - CCNA_00516 0.25 +0.2 531,872 + CCNA_00516 0.25 -1.4 531,872 + CCNA_00516 0.25 -0.0 531,872 + CCNA_00516 0.25 +0.8 531,880 - CCNA_00516 0.26 -0.6 531,880 - CCNA_00516 0.26 -0.0 531,880 - CCNA_00516 0.26 -0.7 531,880 - CCNA_00516 0.26 +1.3 531,882 + CCNA_00516 0.27 +0.8 531,887 + CCNA_00516 0.27 +0.8 531,890 - CCNA_00516 0.28 +0.4 531,890 - CCNA_00516 0.28 -0.4 531,895 - CCNA_00516 0.28 -0.8 531,907 + CCNA_00516 0.30 -2.0 531,915 - CCNA_00516 0.31 +0.8 531,915 - CCNA_00516 0.31 -0.9 531,915 - CCNA_00516 0.31 +0.5 531,939 + CCNA_00516 0.34 +0.6 531,939 + CCNA_00516 0.34 -0.4 531,947 - CCNA_00516 0.35 +0.6 531,947 - CCNA_00516 0.35 +0.6 531,960 + CCNA_00516 0.37 +0.2 531,967 - CCNA_00516 0.38 -0.1 532,014 + CCNA_00516 0.44 +0.3 532,019 + CCNA_00516 0.45 -0.3 532,024 + CCNA_00516 0.45 -1.7 532,037 + CCNA_00516 0.47 -1.0 532,070 - CCNA_00516 0.52 -1.9 532,070 - CCNA_00516 0.52 -0.9 532,087 - CCNA_00516 0.54 +1.0 532,109 - CCNA_00516 0.57 -1.3 532,113 - CCNA_00516 0.57 +0.5 532,177 - CCNA_00516 0.66 +0.5 532,249 - CCNA_00516 0.75 +0.2 532,266 + CCNA_00516 0.78 +1.3 532,272 + CCNA_00516 0.79 -1.4 532,275 - CCNA_00516 0.79 +0.5 532,284 - CCNA_00516 0.80 -0.6 532,298 - CCNA_00516 0.82 +0.8 532,334 + CCNA_00516 0.87 +1.8 532,337 - CCNA_00516 0.87 +1.2 532,355 + CCNA_00516 0.90 +0.3 532,355 + CCNA_00516 0.90 +1.2 532,355 + CCNA_00516 0.90 +1.8 532,363 - -0.4 532,383 + -0.0 532,403 + +1.8 532,461 + +3.3 532,461 + +3.3 532,461 + +3.1 532,474 - +0.3 532,501 - CCNA_00517 0.11 +1.9 532,501 - CCNA_00517 0.11 -0.4 532,501 - CCNA_00517 0.11 +1.8
Or see this region's nucleotide sequence