Experiment: PYE with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00501 and CCNA_00502 are separated by 56 nucleotides CCNA_00502 and CCNA_00503 are separated by 98 nucleotides CCNA_00503 and CCNA_00504 overlap by 4 nucleotides CCNA_00504 and CCNA_00505 are separated by 204 nucleotides
CCNA_00501: CCNA_00501 - glucosyltransferase, at 516,738 to 518,417
_00501
CCNA_00502: CCNA_00502 - glycosyl transferase family protein, at 518,474 to 519,211
_00502
CCNA_00503: CCNA_00503 - hypothetical protein, at 519,310 to 519,732
_00503
CCNA_00504: CCNA_00504 - 23S rRNA methyltransferase, at 519,729 to 520,193
_00504
CCNA_00505: CCNA_00505 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 520,398 to 520,949
_00505
Position (kb) 519
520 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 518.344 kb on + strand at 518.433 kb on - strand at 518.461 kb on + strand at 518.774 kb on + strand, within CCNA_00502 at 519.206 kb on + strand at 519.214 kb on - strand at 519.214 kb on - strand at 519.251 kb on - strand at 519.258 kb on + strand at 519.259 kb on + strand at 519.259 kb on + strand at 519.259 kb on + strand at 519.259 kb on + strand at 519.259 kb on + strand at 519.259 kb on + strand at 519.267 kb on - strand at 519.267 kb on - strand at 519.267 kb on - strand at 519.299 kb on + strand at 519.312 kb on - strand at 519.312 kb on - strand at 519.328 kb on + strand at 519.336 kb on - strand at 519.365 kb on + strand, within CCNA_00503 at 519.373 kb on - strand, within CCNA_00503 at 519.375 kb on + strand, within CCNA_00503 at 519.383 kb on - strand, within CCNA_00503 at 519.384 kb on + strand, within CCNA_00503 at 519.391 kb on - strand, within CCNA_00503 at 519.414 kb on - strand, within CCNA_00503 at 519.435 kb on + strand, within CCNA_00503 at 519.435 kb on + strand, within CCNA_00503 at 519.443 kb on - strand, within CCNA_00503 at 519.448 kb on + strand, within CCNA_00503 at 519.463 kb on - strand, within CCNA_00503 at 519.467 kb on + strand, within CCNA_00503 at 519.475 kb on - strand, within CCNA_00503 at 519.480 kb on + strand, within CCNA_00503 at 519.499 kb on + strand, within CCNA_00503 at 519.582 kb on - strand, within CCNA_00503 at 519.584 kb on + strand, within CCNA_00503 at 519.592 kb on - strand, within CCNA_00503 at 519.592 kb on - strand, within CCNA_00503 at 519.599 kb on + strand, within CCNA_00503 at 519.649 kb on - strand, within CCNA_00503 at 519.655 kb on - strand, within CCNA_00503 at 519.677 kb on + strand, within CCNA_00503 at 519.685 kb on - strand, within CCNA_00503 at 519.767 kb on - strand at 519.772 kb on - strand at 519.774 kb on - strand at 519.815 kb on + strand, within CCNA_00504 at 519.815 kb on + strand, within CCNA_00504 at 519.836 kb on + strand, within CCNA_00504 at 519.856 kb on + strand, within CCNA_00504 at 519.864 kb on - strand, within CCNA_00504 at 519.910 kb on - strand, within CCNA_00504 at 519.910 kb on - strand, within CCNA_00504 at 519.960 kb on + strand, within CCNA_00504 at 519.991 kb on + strand, within CCNA_00504 at 519.991 kb on + strand, within CCNA_00504 at 519.992 kb on - strand, within CCNA_00504 at 520.047 kb on + strand, within CCNA_00504 at 520.047 kb on + strand, within CCNA_00504 at 520.055 kb on - strand, within CCNA_00504 at 520.055 kb on - strand at 520.062 kb on + strand, within CCNA_00504 at 520.062 kb on + strand, within CCNA_00504 at 520.070 kb on - strand, within CCNA_00504 at 520.090 kb on - strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.106 kb on + strand, within CCNA_00504 at 520.107 kb on + strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.114 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.115 kb on - strand, within CCNA_00504 at 520.136 kb on + strand, within CCNA_00504 at 520.136 kb on + strand, within CCNA_00504 at 520.163 kb on - strand at 520.171 kb on + strand at 520.253 kb on + strand at 520.253 kb on - strand at 520.307 kb on - strand at 520.333 kb on - strand at 520.334 kb on - strand at 520.355 kb on - strand at 520.355 kb on - strand at 520.394 kb on - strand at 520.449 kb on - strand at 520.509 kb on + strand, within CCNA_00505 at 520.535 kb on + strand, within CCNA_00505 at 520.543 kb on - strand, within CCNA_00505 at 520.559 kb on + strand, within CCNA_00505 at 520.615 kb on + strand, within CCNA_00505 at 520.615 kb on + strand, within CCNA_00505 at 520.661 kb on + strand, within CCNA_00505
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 5 mM remove 518,344 + +1.5 518,433 - +0.2 518,461 + +0.8 518,774 + CCNA_00502 0.41 +0.5 519,206 + +3.0 519,214 - +0.2 519,214 - -1.6 519,251 - +1.2 519,258 + +2.6 519,259 + -0.3 519,259 + +1.7 519,259 + +1.2 519,259 + +1.5 519,259 + +1.7 519,259 + +0.3 519,267 - +0.8 519,267 - +0.2 519,267 - -0.0 519,299 + +1.7 519,312 - -0.4 519,312 - -0.1 519,328 + -0.6 519,336 - -1.2 519,365 + CCNA_00503 0.13 -1.2 519,373 - CCNA_00503 0.15 +2.8 519,375 + CCNA_00503 0.15 +0.6 519,383 - CCNA_00503 0.17 +0.8 519,384 + CCNA_00503 0.17 +1.2 519,391 - CCNA_00503 0.19 +2.8 519,414 - CCNA_00503 0.25 +0.7 519,435 + CCNA_00503 0.30 +1.3 519,435 + CCNA_00503 0.30 -0.1 519,443 - CCNA_00503 0.31 -0.8 519,448 + CCNA_00503 0.33 +1.2 519,463 - CCNA_00503 0.36 +1.5 519,467 + CCNA_00503 0.37 -1.4 519,475 - CCNA_00503 0.39 -0.9 519,480 + CCNA_00503 0.40 +2.1 519,499 + CCNA_00503 0.45 -0.1 519,582 - CCNA_00503 0.64 +1.8 519,584 + CCNA_00503 0.65 +1.2 519,592 - CCNA_00503 0.67 -0.4 519,592 - CCNA_00503 0.67 +0.5 519,599 + CCNA_00503 0.68 +1.2 519,649 - CCNA_00503 0.80 -0.6 519,655 - CCNA_00503 0.82 -1.1 519,677 + CCNA_00503 0.87 +1.4 519,685 - CCNA_00503 0.89 +0.2 519,767 - +2.8 519,772 - +1.8 519,774 - +0.3 519,815 + CCNA_00504 0.18 +0.2 519,815 + CCNA_00504 0.18 -0.0 519,836 + CCNA_00504 0.23 -0.0 519,856 + CCNA_00504 0.27 +2.8 519,864 - CCNA_00504 0.29 +0.1 519,910 - CCNA_00504 0.39 +0.2 519,910 - CCNA_00504 0.39 +1.6 519,960 + CCNA_00504 0.50 -0.9 519,991 + CCNA_00504 0.56 -0.8 519,991 + CCNA_00504 0.56 -1.1 519,992 - CCNA_00504 0.57 -1.1 520,047 + CCNA_00504 0.68 +0.5 520,047 + CCNA_00504 0.68 +0.2 520,055 - CCNA_00504 0.70 +0.7 520,055 - +1.8 520,062 + CCNA_00504 0.72 -1.3 520,062 + CCNA_00504 0.72 -1.9 520,070 - CCNA_00504 0.73 +0.8 520,090 - CCNA_00504 0.78 +0.8 520,106 + CCNA_00504 0.81 -0.6 520,106 + CCNA_00504 0.81 -0.9 520,106 + CCNA_00504 0.81 -0.8 520,106 + CCNA_00504 0.81 +0.1 520,106 + CCNA_00504 0.81 -0.5 520,106 + CCNA_00504 0.81 +1.5 520,106 + CCNA_00504 0.81 -1.4 520,106 + CCNA_00504 0.81 -2.0 520,106 + CCNA_00504 0.81 -1.1 520,107 + CCNA_00504 0.81 -0.4 520,114 - CCNA_00504 0.83 +0.3 520,114 - CCNA_00504 0.83 +0.1 520,114 - CCNA_00504 0.83 -0.9 520,114 - CCNA_00504 0.83 -2.0 520,114 - CCNA_00504 0.83 -1.3 520,114 - CCNA_00504 0.83 -0.7 520,114 - CCNA_00504 0.83 +0.2 520,115 - CCNA_00504 0.83 +1.8 520,115 - CCNA_00504 0.83 -1.7 520,115 - CCNA_00504 0.83 +1.2 520,115 - CCNA_00504 0.83 -0.0 520,115 - CCNA_00504 0.83 -1.1 520,115 - CCNA_00504 0.83 +1.2 520,136 + CCNA_00504 0.88 -2.2 520,136 + CCNA_00504 0.88 -0.0 520,163 - +0.8 520,171 + +0.6 520,253 + +4.0 520,253 - -0.4 520,307 - +0.2 520,333 - +0.3 520,334 - +1.3 520,355 - +0.5 520,355 - -0.2 520,394 - -0.9 520,449 - -0.0 520,509 + CCNA_00505 0.20 +2.8 520,535 + CCNA_00505 0.25 +2.7 520,543 - CCNA_00505 0.26 +0.8 520,559 + CCNA_00505 0.29 +2.7 520,615 + CCNA_00505 0.39 +2.9 520,615 + CCNA_00505 0.39 +3.5 520,661 + CCNA_00505 0.48 +3.2
Or see this region's nucleotide sequence