Experiment: rhizosphere EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11740 and ABZR87_RS11745 are separated by 17 nucleotides ABZR87_RS11745 and ABZR87_RS11750 are separated by 10 nucleotides ABZR87_RS11750 and ABZR87_RS11755 are separated by 37 nucleotides ABZR87_RS11755 and ABZR87_RS11760 are separated by 14 nucleotides ABZR87_RS11760 and ABZR87_RS11765 are separated by 60 nucleotides
ABZR87_RS11740: ABZR87_RS11740 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 2,489,230 to 2,490,132
_RS11740
ABZR87_RS11745: ABZR87_RS11745 - class IV adenylate cyclase, at 2,490,150 to 2,490,668
_RS11745
ABZR87_RS11750: ABZR87_RS11750 - succinyl-diaminopimelate desuccinylase, at 2,490,679 to 2,491,830
_RS11750
ABZR87_RS11755: ABZR87_RS11755 - arsenate reductase, at 2,491,868 to 2,492,212
_RS11755
ABZR87_RS11760: ABZR87_RS11760 - membrane protein, at 2,492,227 to 2,492,640
_RS11760
ABZR87_RS11765: ABZR87_RS11765 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,492,701 to 2,493,528
_RS11765
Position (kb)
2490
2491
2492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2489.720 kb on - strand, within ABZR87_RS11740 at 2489.822 kb on + strand, within ABZR87_RS11740 at 2489.822 kb on + strand, within ABZR87_RS11740 at 2489.823 kb on - strand, within ABZR87_RS11740 at 2489.861 kb on + strand, within ABZR87_RS11740 at 2489.861 kb on + strand, within ABZR87_RS11740 at 2489.862 kb on - strand, within ABZR87_RS11740 at 2489.862 kb on - strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.996 kb on + strand, within ABZR87_RS11740 at 2489.997 kb on - strand, within ABZR87_RS11740 at 2490.072 kb on - strand at 2490.072 kb on - strand at 2490.181 kb on + strand at 2490.181 kb on + strand at 2490.181 kb on + strand at 2490.246 kb on + strand, within ABZR87_RS11745 at 2490.269 kb on + strand, within ABZR87_RS11745 at 2490.353 kb on - strand, within ABZR87_RS11745 at 2490.448 kb on + strand, within ABZR87_RS11745 at 2490.449 kb on - strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.487 kb on + strand, within ABZR87_RS11745 at 2490.488 kb on - strand, within ABZR87_RS11745 at 2490.488 kb on - strand, within ABZR87_RS11745 at 2491.870 kb on - strand at 2491.885 kb on - strand at 2492.133 kb on + strand, within ABZR87_RS11755 at 2492.134 kb on - strand, within ABZR87_RS11755 at 2492.436 kb on + strand, within ABZR87_RS11760 at 2492.676 kb on + strand at 2492.676 kb on + strand at 2492.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 2; outgrowth in LB remove 2,489,720 - ABZR87_RS11740 0.54 -0.6 2,489,822 + ABZR87_RS11740 0.66 +0.8 2,489,822 + ABZR87_RS11740 0.66 -0.9 2,489,823 - ABZR87_RS11740 0.66 -0.1 2,489,861 + ABZR87_RS11740 0.70 -1.2 2,489,861 + ABZR87_RS11740 0.70 +0.8 2,489,862 - ABZR87_RS11740 0.70 +0.3 2,489,862 - ABZR87_RS11740 0.70 -0.3 2,489,996 + ABZR87_RS11740 0.85 -0.1 2,489,996 + ABZR87_RS11740 0.85 -0.3 2,489,996 + ABZR87_RS11740 0.85 +0.9 2,489,997 - ABZR87_RS11740 0.85 +2.0 2,490,072 - -0.1 2,490,072 - +0.4 2,490,181 + +0.6 2,490,181 + +0.3 2,490,181 + -0.0 2,490,246 + ABZR87_RS11745 0.18 +0.6 2,490,269 + ABZR87_RS11745 0.23 -0.4 2,490,353 - ABZR87_RS11745 0.39 +0.1 2,490,448 + ABZR87_RS11745 0.57 -0.5 2,490,449 - ABZR87_RS11745 0.58 +0.5 2,490,487 + ABZR87_RS11745 0.65 +1.0 2,490,487 + ABZR87_RS11745 0.65 -3.1 2,490,487 + ABZR87_RS11745 0.65 +0.1 2,490,488 - ABZR87_RS11745 0.65 -0.1 2,490,488 - ABZR87_RS11745 0.65 +0.5 2,491,870 - +0.2 2,491,885 - +1.1 2,492,133 + ABZR87_RS11755 0.77 -0.7 2,492,134 - ABZR87_RS11755 0.77 -0.1 2,492,436 + ABZR87_RS11760 0.50 +0.1 2,492,676 + +0.0 2,492,676 + +0.2 2,492,703 - -0.9
Or see this region's nucleotide sequence