Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07735 and MMJJ_RS07740 are separated by 120 nucleotides MMJJ_RS07740 and MMJJ_RS07745 overlap by 4 nucleotides MMJJ_RS07745 and MMJJ_RS07750 are separated by 112 nucleotides MMJJ_RS07750 and MMJJ_RS07755 are separated by 37 nucleotides MMJJ_RS07755 and MMJJ_RS07760 are separated by 54 nucleotides
MMJJ_RS07735: MMJJ_RS07735 - tRNA-Gly, at 1,419,000 to 1,419,072
_RS07735
MMJJ_RS07740: MMJJ_RS07740 - DUF2334 domain-containing protein, at 1,419,193 to 1,419,993
_RS07740
MMJJ_RS07745: MMJJ_RS07745 - DUF5814 domain-containing protein, at 1,419,990 to 1,422,425
_RS07745
MMJJ_RS07750: MMJJ_RS07750 - class III signal peptide-containing protein, at 1,422,538 to 1,422,741
_RS07750
MMJJ_RS07755: MMJJ_RS07755 - flagellar hook-basal body complex protein FliE, at 1,422,779 to 1,423,330
_RS07755
MMJJ_RS07760: MMJJ_RS07760 - GNAT family N-acetyltransferase, at 1,423,385 to 1,423,891
_RS07760
Position (kb)
1419
1420
1421
1422
1423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1419.044 kb on - strand, within MMJJ_RS07735 at 1419.080 kb on + strand at 1419.080 kb on + strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.129 kb on - strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.130 kb on + strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.131 kb on - strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.132 kb on + strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.133 kb on - strand at 1419.148 kb on - strand at 1419.149 kb on + strand at 1419.158 kb on - strand at 1419.159 kb on + strand at 1419.162 kb on - strand at 1419.163 kb on + strand at 1419.165 kb on + strand at 1419.175 kb on + strand at 1419.184 kb on - strand at 1419.184 kb on - strand at 1419.185 kb on + strand at 1419.185 kb on + strand at 1419.228 kb on + strand at 1419.319 kb on + strand, within MMJJ_RS07740 at 1419.328 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.359 kb on - strand, within MMJJ_RS07740 at 1419.360 kb on + strand, within MMJJ_RS07740 at 1419.364 kb on + strand, within MMJJ_RS07740 at 1419.370 kb on - strand, within MMJJ_RS07740 at 1419.371 kb on + strand, within MMJJ_RS07740 at 1419.425 kb on + strand, within MMJJ_RS07740 at 1419.484 kb on - strand, within MMJJ_RS07740 at 1419.485 kb on + strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.534 kb on - strand, within MMJJ_RS07740 at 1419.613 kb on + strand, within MMJJ_RS07740 at 1419.680 kb on - strand, within MMJJ_RS07740 at 1419.695 kb on + strand, within MMJJ_RS07740 at 1419.727 kb on + strand, within MMJJ_RS07740 at 1419.727 kb on + strand, within MMJJ_RS07740 at 1419.731 kb on + strand, within MMJJ_RS07740 at 1419.732 kb on - strand, within MMJJ_RS07740 at 1419.754 kb on + strand, within MMJJ_RS07740 at 1419.754 kb on + strand, within MMJJ_RS07740 at 1419.791 kb on - strand, within MMJJ_RS07740 at 1419.811 kb on - strand, within MMJJ_RS07740 at 1419.906 kb on - strand, within MMJJ_RS07740 at 1419.951 kb on - strand at 1419.956 kb on + strand at 1420.058 kb on + strand at 1420.092 kb on + strand at 1420.112 kb on + strand at 1420.121 kb on - strand at 1420.121 kb on - strand at 1420.231 kb on + strand at 1420.231 kb on + strand at 1420.232 kb on - strand at 1420.233 kb on + strand at 1420.326 kb on - strand, within MMJJ_RS07745 at 1420.326 kb on - strand, within MMJJ_RS07745 at 1420.329 kb on - strand, within MMJJ_RS07745 at 1420.329 kb on - strand, within MMJJ_RS07745 at 1420.362 kb on + strand, within MMJJ_RS07745 at 1420.409 kb on - strand, within MMJJ_RS07745 at 1420.409 kb on - strand, within MMJJ_RS07745 at 1420.410 kb on + strand, within MMJJ_RS07745 at 1420.411 kb on - strand, within MMJJ_RS07745 at 1420.411 kb on - strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.412 kb on + strand, within MMJJ_RS07745 at 1420.438 kb on + strand, within MMJJ_RS07745 at 1420.493 kb on - strand, within MMJJ_RS07745 at 1420.498 kb on - strand, within MMJJ_RS07745 at 1420.538 kb on - strand, within MMJJ_RS07745 at 1420.539 kb on + strand, within MMJJ_RS07745 at 1420.590 kb on - strand, within MMJJ_RS07745 at 1420.652 kb on - strand, within MMJJ_RS07745 at 1420.652 kb on - strand, within MMJJ_RS07745 at 1420.653 kb on + strand, within MMJJ_RS07745 at 1420.653 kb on + strand, within MMJJ_RS07745 at 1420.659 kb on + strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.683 kb on - strand, within MMJJ_RS07745 at 1420.684 kb on + strand, within MMJJ_RS07745 at 1420.684 kb on + strand, within MMJJ_RS07745 at 1420.724 kb on + strand, within MMJJ_RS07745 at 1420.755 kb on - strand, within MMJJ_RS07745 at 1420.804 kb on - strand, within MMJJ_RS07745 at 1420.933 kb on - strand, within MMJJ_RS07745 at 1420.986 kb on - strand, within MMJJ_RS07745 at 1420.986 kb on - strand, within MMJJ_RS07745 at 1420.989 kb on - strand, within MMJJ_RS07745 at 1420.989 kb on - strand, within MMJJ_RS07745 at 1421.045 kb on + strand, within MMJJ_RS07745 at 1421.065 kb on - strand, within MMJJ_RS07745 at 1421.088 kb on + strand, within MMJJ_RS07745 at 1421.107 kb on - strand, within MMJJ_RS07745 at 1421.137 kb on + strand, within MMJJ_RS07745 at 1421.177 kb on - strand, within MMJJ_RS07745 at 1421.341 kb on - strand, within MMJJ_RS07745 at 1421.343 kb on - strand, within MMJJ_RS07745 at 1421.361 kb on - strand, within MMJJ_RS07745 at 1421.460 kb on - strand, within MMJJ_RS07745 at 1421.482 kb on - strand, within MMJJ_RS07745 at 1421.487 kb on - strand, within MMJJ_RS07745 at 1421.487 kb on - strand, within MMJJ_RS07745 at 1421.488 kb on + strand, within MMJJ_RS07745 at 1421.508 kb on - strand, within MMJJ_RS07745 at 1421.509 kb on + strand, within MMJJ_RS07745 at 1421.509 kb on + strand, within MMJJ_RS07745 at 1421.509 kb on + strand, within MMJJ_RS07745 at 1421.509 kb on + strand, within MMJJ_RS07745 at 1421.609 kb on + strand, within MMJJ_RS07745 at 1421.612 kb on - strand, within MMJJ_RS07745 at 1421.612 kb on - strand, within MMJJ_RS07745 at 1421.653 kb on + strand, within MMJJ_RS07745 at 1421.694 kb on + strand, within MMJJ_RS07745 at 1421.750 kb on - strand, within MMJJ_RS07745 at 1421.750 kb on - strand, within MMJJ_RS07745 at 1421.750 kb on - strand, within MMJJ_RS07745 at 1421.755 kb on + strand, within MMJJ_RS07745 at 1421.811 kb on - strand, within MMJJ_RS07745 at 1421.812 kb on + strand, within MMJJ_RS07745 at 1421.874 kb on - strand, within MMJJ_RS07745 at 1421.874 kb on - strand, within MMJJ_RS07745 at 1421.923 kb on - strand, within MMJJ_RS07745 at 1421.982 kb on + strand, within MMJJ_RS07745 at 1422.036 kb on - strand, within MMJJ_RS07745 at 1422.036 kb on - strand, within MMJJ_RS07745 at 1422.036 kb on - strand, within MMJJ_RS07745 at 1422.036 kb on - strand, within MMJJ_RS07745 at 1422.101 kb on - strand, within MMJJ_RS07745 at 1422.155 kb on - strand, within MMJJ_RS07745 at 1422.449 kb on + strand at 1422.462 kb on - strand at 1422.468 kb on - strand at 1422.476 kb on - strand at 1422.502 kb on + strand at 1422.503 kb on - strand at 1422.506 kb on + strand at 1422.506 kb on + strand at 1422.506 kb on + strand at 1422.510 kb on - strand at 1422.516 kb on - strand at 1422.516 kb on - strand at 1422.516 kb on - strand at 1422.516 kb on - strand at 1422.516 kb on - strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.517 kb on + strand at 1422.518 kb on - strand at 1422.518 kb on - strand at 1422.518 kb on - strand at 1422.519 kb on + strand at 1422.519 kb on + strand at 1422.519 kb on + strand at 1422.521 kb on + strand at 1422.521 kb on + strand at 1422.521 kb on + strand at 1422.535 kb on + strand at 1422.618 kb on - strand, within MMJJ_RS07750 at 1422.636 kb on + strand, within MMJJ_RS07750 at 1422.653 kb on - strand, within MMJJ_RS07750 at 1422.654 kb on + strand, within MMJJ_RS07750 at 1422.739 kb on - strand at 1422.747 kb on - strand at 1423.318 kb on - strand at 1423.367 kb on - strand at 1423.379 kb on + strand at 1423.381 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 1,419,044 - MMJJ_RS07735 0.60 +1.7 1,419,080 + +0.4 1,419,080 + +0.3 1,419,129 - +1.7 1,419,129 - +0.3 1,419,129 - +0.6 1,419,130 + +0.7 1,419,130 + -3.2 1,419,130 + -1.2 1,419,130 + +1.8 1,419,130 + +2.3 1,419,131 - -0.0 1,419,131 - +0.3 1,419,131 - +0.4 1,419,131 - +1.6 1,419,132 + -0.6 1,419,132 + -1.8 1,419,132 + -0.2 1,419,132 + -0.3 1,419,132 + +0.6 1,419,132 + -1.7 1,419,132 + +0.4 1,419,133 - +0.5 1,419,133 - -1.4 1,419,133 - +0.7 1,419,133 - +4.1 1,419,148 - -0.4 1,419,149 + +1.0 1,419,158 - +1.5 1,419,159 + -0.2 1,419,162 - +2.7 1,419,163 + -0.6 1,419,165 + +1.1 1,419,175 + +0.4 1,419,184 - -1.4 1,419,184 - -0.9 1,419,185 + -0.2 1,419,185 + +1.1 1,419,228 + -0.4 1,419,319 + MMJJ_RS07740 0.16 +0.1 1,419,328 - MMJJ_RS07740 0.17 -1.0 1,419,359 - MMJJ_RS07740 0.21 -0.3 1,419,359 - MMJJ_RS07740 0.21 +0.4 1,419,360 + MMJJ_RS07740 0.21 +1.0 1,419,364 + MMJJ_RS07740 0.21 +0.8 1,419,370 - MMJJ_RS07740 0.22 -0.3 1,419,371 + MMJJ_RS07740 0.22 +0.2 1,419,425 + MMJJ_RS07740 0.29 -0.3 1,419,484 - MMJJ_RS07740 0.36 +2.1 1,419,485 + MMJJ_RS07740 0.36 +0.6 1,419,534 - MMJJ_RS07740 0.43 +1.0 1,419,534 - MMJJ_RS07740 0.43 +0.8 1,419,613 + MMJJ_RS07740 0.52 +0.6 1,419,680 - MMJJ_RS07740 0.61 -0.4 1,419,695 + MMJJ_RS07740 0.63 -0.9 1,419,727 + MMJJ_RS07740 0.67 +1.2 1,419,727 + MMJJ_RS07740 0.67 +0.3 1,419,731 + MMJJ_RS07740 0.67 -3.3 1,419,732 - MMJJ_RS07740 0.67 -0.5 1,419,754 + MMJJ_RS07740 0.70 -0.4 1,419,754 + MMJJ_RS07740 0.70 -0.6 1,419,791 - MMJJ_RS07740 0.75 -1.1 1,419,811 - MMJJ_RS07740 0.77 +0.7 1,419,906 - MMJJ_RS07740 0.89 -0.4 1,419,951 - -0.6 1,419,956 + +1.3 1,420,058 + +1.0 1,420,092 + +0.5 1,420,112 + +1.9 1,420,121 - +1.2 1,420,121 - -1.7 1,420,231 + +2.5 1,420,231 + -0.3 1,420,232 - +3.4 1,420,233 + +1.3 1,420,326 - MMJJ_RS07745 0.14 +2.4 1,420,326 - MMJJ_RS07745 0.14 +2.9 1,420,329 - MMJJ_RS07745 0.14 -0.1 1,420,329 - MMJJ_RS07745 0.14 +3.3 1,420,362 + MMJJ_RS07745 0.15 +0.3 1,420,409 - MMJJ_RS07745 0.17 +2.3 1,420,409 - MMJJ_RS07745 0.17 +0.8 1,420,410 + MMJJ_RS07745 0.17 +1.2 1,420,411 - MMJJ_RS07745 0.17 +0.8 1,420,411 - MMJJ_RS07745 0.17 +1.5 1,420,412 + MMJJ_RS07745 0.17 +1.5 1,420,412 + MMJJ_RS07745 0.17 -0.1 1,420,412 + MMJJ_RS07745 0.17 +2.2 1,420,438 + MMJJ_RS07745 0.18 +2.8 1,420,493 - MMJJ_RS07745 0.21 +3.1 1,420,498 - MMJJ_RS07745 0.21 +0.9 1,420,538 - MMJJ_RS07745 0.22 +2.2 1,420,539 + MMJJ_RS07745 0.23 +0.5 1,420,590 - MMJJ_RS07745 0.25 +0.1 1,420,652 - MMJJ_RS07745 0.27 +1.4 1,420,652 - MMJJ_RS07745 0.27 +1.3 1,420,653 + MMJJ_RS07745 0.27 -0.2 1,420,653 + MMJJ_RS07745 0.27 +0.5 1,420,659 + MMJJ_RS07745 0.27 +1.6 1,420,683 - MMJJ_RS07745 0.28 +2.4 1,420,683 - MMJJ_RS07745 0.28 +2.3 1,420,683 - MMJJ_RS07745 0.28 +3.6 1,420,684 + MMJJ_RS07745 0.28 +0.2 1,420,684 + MMJJ_RS07745 0.28 +2.5 1,420,724 + MMJJ_RS07745 0.30 +2.2 1,420,755 - MMJJ_RS07745 0.31 +0.0 1,420,804 - MMJJ_RS07745 0.33 -0.4 1,420,933 - MMJJ_RS07745 0.39 +1.1 1,420,986 - MMJJ_RS07745 0.41 -1.5 1,420,986 - MMJJ_RS07745 0.41 +1.3 1,420,989 - MMJJ_RS07745 0.41 +2.6 1,420,989 - MMJJ_RS07745 0.41 +1.8 1,421,045 + MMJJ_RS07745 0.43 +0.9 1,421,065 - MMJJ_RS07745 0.44 +0.5 1,421,088 + MMJJ_RS07745 0.45 -0.3 1,421,107 - MMJJ_RS07745 0.46 +0.7 1,421,137 + MMJJ_RS07745 0.47 -0.4 1,421,177 - MMJJ_RS07745 0.49 +3.3 1,421,341 - MMJJ_RS07745 0.55 -0.6 1,421,343 - MMJJ_RS07745 0.56 +0.6 1,421,361 - MMJJ_RS07745 0.56 +0.3 1,421,460 - MMJJ_RS07745 0.60 -2.0 1,421,482 - MMJJ_RS07745 0.61 +1.3 1,421,487 - MMJJ_RS07745 0.61 -0.1 1,421,487 - MMJJ_RS07745 0.61 +0.4 1,421,488 + MMJJ_RS07745 0.61 +1.8 1,421,508 - MMJJ_RS07745 0.62 -0.5 1,421,509 + MMJJ_RS07745 0.62 +1.1 1,421,509 + MMJJ_RS07745 0.62 -0.3 1,421,509 + MMJJ_RS07745 0.62 +0.9 1,421,509 + MMJJ_RS07745 0.62 +2.6 1,421,609 + MMJJ_RS07745 0.66 +1.4 1,421,612 - MMJJ_RS07745 0.67 -0.2 1,421,612 - MMJJ_RS07745 0.67 +1.2 1,421,653 + MMJJ_RS07745 0.68 +0.5 1,421,694 + MMJJ_RS07745 0.70 -1.7 1,421,750 - MMJJ_RS07745 0.72 -1.0 1,421,750 - MMJJ_RS07745 0.72 +1.4 1,421,750 - MMJJ_RS07745 0.72 -2.3 1,421,755 + MMJJ_RS07745 0.72 +0.8 1,421,811 - MMJJ_RS07745 0.75 +0.6 1,421,812 + MMJJ_RS07745 0.75 +1.1 1,421,874 - MMJJ_RS07745 0.77 +1.7 1,421,874 - MMJJ_RS07745 0.77 +1.5 1,421,923 - MMJJ_RS07745 0.79 -0.2 1,421,982 + MMJJ_RS07745 0.82 +0.5 1,422,036 - MMJJ_RS07745 0.84 +0.6 1,422,036 - MMJJ_RS07745 0.84 +2.0 1,422,036 - MMJJ_RS07745 0.84 -2.9 1,422,036 - MMJJ_RS07745 0.84 +1.9 1,422,101 - MMJJ_RS07745 0.87 +1.4 1,422,155 - MMJJ_RS07745 0.89 +2.3 1,422,449 + +1.4 1,422,462 - -0.5 1,422,468 - +0.4 1,422,476 - +2.4 1,422,502 + -0.7 1,422,503 - -0.3 1,422,506 + +1.5 1,422,506 + -0.1 1,422,506 + -0.7 1,422,510 - -3.0 1,422,516 - +0.2 1,422,516 - -0.9 1,422,516 - +0.3 1,422,516 - -0.6 1,422,516 - -0.1 1,422,517 + -0.5 1,422,517 + +2.3 1,422,517 + +1.8 1,422,517 + -0.2 1,422,517 + +1.7 1,422,517 + +0.8 1,422,517 + +0.5 1,422,517 + +0.4 1,422,518 - -0.6 1,422,518 - -0.5 1,422,518 - +0.5 1,422,519 + +0.3 1,422,519 + +2.1 1,422,519 + +1.2 1,422,521 + +0.2 1,422,521 + -0.6 1,422,521 + -1.7 1,422,535 + +2.1 1,422,618 - MMJJ_RS07750 0.39 +1.8 1,422,636 + MMJJ_RS07750 0.48 +0.4 1,422,653 - MMJJ_RS07750 0.56 +1.1 1,422,654 + MMJJ_RS07750 0.57 -2.0 1,422,739 - +0.4 1,422,747 - -0.3 1,423,318 - +1.4 1,423,367 - -2.1 1,423,379 + -0.5 1,423,381 + -1.0
Or see this region's nucleotide sequence