Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00180

Experiment: H2 N-free with 2mM Adenosine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00170 and MMJJ_RS00175 are separated by 19 nucleotidesMMJJ_RS00175 and MMJJ_RS00180 are separated by 1 nucleotidesMMJJ_RS00180 and MMJJ_RS00185 are separated by 46 nucleotides MMJJ_RS00170: MMJJ_RS00170 - thioredoxin family protein, at 31,537 to 31,884 _RS00170 MMJJ_RS00175: MMJJ_RS00175 - cytochrome c biogenesis CcdA family protein, at 31,904 to 32,557 _RS00175 MMJJ_RS00180: MMJJ_RS00180 - DNA topoisomerase I, at 32,559 to 35,030 _RS00180 MMJJ_RS00185: MMJJ_RS00185 - succinate--CoA ligase subunit alpha, at 35,077 to 35,940 _RS00185 Position (kb) 32 33 34 35 36Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7at 31.562 kb on - strandat 31.562 kb on - strandat 31.566 kb on - strandat 31.566 kb on - strandat 31.567 kb on + strandat 31.567 kb on + strandat 31.568 kb on - strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.593 kb on - strand, within MMJJ_RS00170at 31.594 kb on + strand, within MMJJ_RS00170at 31.594 kb on + strand, within MMJJ_RS00170at 31.616 kb on - strand, within MMJJ_RS00170at 31.616 kb on - strand, within MMJJ_RS00170at 31.620 kb on - strand, within MMJJ_RS00170at 31.621 kb on + strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.725 kb on + strand, within MMJJ_RS00170at 31.734 kb on + strand, within MMJJ_RS00170at 31.768 kb on - strand, within MMJJ_RS00170at 31.773 kb on - strand, within MMJJ_RS00170at 31.850 kb on - strandat 31.853 kb on - strandat 31.890 kb on + strandat 31.962 kb on - strandat 31.971 kb on - strand, within MMJJ_RS00175at 31.972 kb on + strand, within MMJJ_RS00175at 31.972 kb on + strand, within MMJJ_RS00175at 31.984 kb on + strand, within MMJJ_RS00175at 31.988 kb on - strand, within MMJJ_RS00175at 31.988 kb on - strand, within MMJJ_RS00175at 31.989 kb on + strand, within MMJJ_RS00175at 31.990 kb on - strand, within MMJJ_RS00175at 31.991 kb on + strand, within MMJJ_RS00175at 31.991 kb on + strand, within MMJJ_RS00175at 32.005 kb on - strand, within MMJJ_RS00175at 32.045 kb on + strand, within MMJJ_RS00175at 32.129 kb on - strand, within MMJJ_RS00175at 32.248 kb on - strand, within MMJJ_RS00175at 32.249 kb on + strand, within MMJJ_RS00175at 32.256 kb on - strand, within MMJJ_RS00175at 32.272 kb on + strand, within MMJJ_RS00175at 32.314 kb on - strand, within MMJJ_RS00175at 32.314 kb on - strand, within MMJJ_RS00175at 32.315 kb on + strand, within MMJJ_RS00175at 32.316 kb on - strand, within MMJJ_RS00175at 32.316 kb on - strand, within MMJJ_RS00175at 32.317 kb on + strand, within MMJJ_RS00175at 32.375 kb on - strand, within MMJJ_RS00175at 32.402 kb on - strand, within MMJJ_RS00175at 32.430 kb on - strand, within MMJJ_RS00175at 32.431 kb on + strand, within MMJJ_RS00175at 32.466 kb on - strand, within MMJJ_RS00175at 32.482 kb on + strand, within MMJJ_RS00175at 32.495 kb on + strandat 32.502 kb on + strandat 32.502 kb on + strandat 32.503 kb on - strandat 32.503 kb on - strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.515 kb on - strandat 32.516 kb on + strandat 32.518 kb on + strandat 32.545 kb on + strandat 32.545 kb on + strandat 32.560 kb on - strandat 32.595 kb on + strandat 32.636 kb on - strandat 32.636 kb on - strandat 32.729 kb on - strandat 32.748 kb on - strandat 32.766 kb on + strandat 32.819 kb on - strand, within MMJJ_RS00180at 32.820 kb on + strand, within MMJJ_RS00180at 32.822 kb on + strand, within MMJJ_RS00180at 32.896 kb on - strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.900 kb on - strand, within MMJJ_RS00180at 32.901 kb on + strand, within MMJJ_RS00180at 33.041 kb on - strand, within MMJJ_RS00180at 33.074 kb on - strand, within MMJJ_RS00180at 33.123 kb on - strand, within MMJJ_RS00180at 33.159 kb on + strand, within MMJJ_RS00180at 33.175 kb on - strand, within MMJJ_RS00180at 33.213 kb on + strand, within MMJJ_RS00180at 33.243 kb on + strand, within MMJJ_RS00180at 33.256 kb on + strand, within MMJJ_RS00180at 33.262 kb on + strand, within MMJJ_RS00180at 33.270 kb on + strand, within MMJJ_RS00180at 33.270 kb on + strand, within MMJJ_RS00180at 33.734 kb on + strand, within MMJJ_RS00180at 33.737 kb on + strand, within MMJJ_RS00180at 33.739 kb on - strand, within MMJJ_RS00180at 33.740 kb on + strand, within MMJJ_RS00180at 34.006 kb on - strand, within MMJJ_RS00180at 34.058 kb on + strand, within MMJJ_RS00180at 34.058 kb on + strand, within MMJJ_RS00180at 34.780 kb on - strand, within MMJJ_RS00180at 34.874 kb on + strandat 34.904 kb on - strandat 34.904 kb on - strandat 34.923 kb on - strandat 35.050 kb on + strandat 35.055 kb on - strandat 35.056 kb on + strandat 35.096 kb on + strandat 35.588 kb on + strand, within MMJJ_RS00185at 36.008 kb on - strandat 36.018 kb on - strandat 36.023 kb on + strandat 36.023 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine
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31,562 - -0.1
31,562 - +0.5
31,566 - -0.6
31,566 - +0.0
31,567 + -0.2
31,567 + -0.1
31,568 - -0.2
31,569 + -0.3
31,569 + -0.4
31,569 + -0.8
31,569 + -0.9
31,593 - MMJJ_RS00170 0.16 -1.5
31,594 + MMJJ_RS00170 0.16 +2.3
31,594 + MMJJ_RS00170 0.16 +0.7
31,616 - MMJJ_RS00170 0.23 +7.0
31,616 - MMJJ_RS00170 0.23 +0.8
31,620 - MMJJ_RS00170 0.24 +2.5
31,621 + MMJJ_RS00170 0.24 +2.1
31,722 - MMJJ_RS00170 0.53 -0.3
31,722 - MMJJ_RS00170 0.53 -0.5
31,722 - MMJJ_RS00170 0.53 +0.1
31,723 + MMJJ_RS00170 0.53 -1.6
31,723 + MMJJ_RS00170 0.53 +1.6
31,723 + MMJJ_RS00170 0.53 -0.5
31,725 + MMJJ_RS00170 0.54 -0.2
31,734 + MMJJ_RS00170 0.57 -1.5
31,768 - MMJJ_RS00170 0.66 -0.9
31,773 - MMJJ_RS00170 0.68 +1.5
31,850 - -0.4
31,853 - -0.3
31,890 + +1.5
31,962 - +0.1
31,971 - MMJJ_RS00175 0.10 -2.0
31,972 + MMJJ_RS00175 0.10 +0.1
31,972 + MMJJ_RS00175 0.10 -0.2
31,984 + MMJJ_RS00175 0.12 -0.5
31,988 - MMJJ_RS00175 0.13 +1.5
31,988 - MMJJ_RS00175 0.13 +0.1
31,989 + MMJJ_RS00175 0.13 +0.3
31,990 - MMJJ_RS00175 0.13 +0.7
31,991 + MMJJ_RS00175 0.13 -0.9
31,991 + MMJJ_RS00175 0.13 -0.4
32,005 - MMJJ_RS00175 0.15 -1.2
32,045 + MMJJ_RS00175 0.22 -3.6
32,129 - MMJJ_RS00175 0.34 +0.1
32,248 - MMJJ_RS00175 0.53 +2.4
32,249 + MMJJ_RS00175 0.53 -2.2
32,256 - MMJJ_RS00175 0.54 +1.5
32,272 + MMJJ_RS00175 0.56 +0.2
32,314 - MMJJ_RS00175 0.63 -0.5
32,314 - MMJJ_RS00175 0.63 -0.6
32,315 + MMJJ_RS00175 0.63 -1.0
32,316 - MMJJ_RS00175 0.63 -3.4
32,316 - MMJJ_RS00175 0.63 -1.4
32,317 + MMJJ_RS00175 0.63 -2.5
32,375 - MMJJ_RS00175 0.72 +1.2
32,402 - MMJJ_RS00175 0.76 -0.8
32,430 - MMJJ_RS00175 0.80 +3.7
32,431 + MMJJ_RS00175 0.81 -1.6
32,466 - MMJJ_RS00175 0.86 -0.0
32,482 + MMJJ_RS00175 0.88 -2.4
32,495 + +0.3
32,502 + -0.8
32,502 + +2.5
32,503 - +0.7
32,503 - -0.1
32,504 + +0.9
32,504 + +1.1
32,504 + -1.1
32,504 + -2.1
32,515 - +2.3
32,516 + +3.2
32,518 + -1.1
32,545 + +0.2
32,545 + +1.0
32,560 - -0.1
32,595 + +0.0
32,636 - -0.9
32,636 - +1.5
32,729 - -0.2
32,748 - -0.4
32,766 + -2.1
32,819 - MMJJ_RS00180 0.11 -2.8
32,820 + MMJJ_RS00180 0.11 -0.5
32,822 + MMJJ_RS00180 0.11 +0.4
32,896 - MMJJ_RS00180 0.14 -0.2
32,897 + MMJJ_RS00180 0.14 -2.9
32,897 + MMJJ_RS00180 0.14 -2.3
32,897 + MMJJ_RS00180 0.14 -0.8
32,900 - MMJJ_RS00180 0.14 +1.1
32,901 + MMJJ_RS00180 0.14 -2.8
33,041 - MMJJ_RS00180 0.19 -1.0
33,074 - MMJJ_RS00180 0.21 -1.3
33,123 - MMJJ_RS00180 0.23 +1.1
33,159 + MMJJ_RS00180 0.24 -2.0
33,175 - MMJJ_RS00180 0.25 +1.5
33,213 + MMJJ_RS00180 0.26 +0.4
33,243 + MMJJ_RS00180 0.28 -0.2
33,256 + MMJJ_RS00180 0.28 +0.4
33,262 + MMJJ_RS00180 0.28 -0.6
33,270 + MMJJ_RS00180 0.29 -1.6
33,270 + MMJJ_RS00180 0.29 -0.3
33,734 + MMJJ_RS00180 0.48 -0.2
33,737 + MMJJ_RS00180 0.48 +0.1
33,739 - MMJJ_RS00180 0.48 +0.1
33,740 + MMJJ_RS00180 0.48 +0.9
34,006 - MMJJ_RS00180 0.59 +0.6
34,058 + MMJJ_RS00180 0.61 -1.6
34,058 + MMJJ_RS00180 0.61 -0.5
34,780 - MMJJ_RS00180 0.90 +1.4
34,874 + -1.0
34,904 - +0.8
34,904 - -0.2
34,923 - +1.4
35,050 + -2.1
35,055 - -2.0
35,056 + +0.2
35,096 + +0.8
35,588 + MMJJ_RS00185 0.59 -0.7
36,008 - +0.6
36,018 - +1.1
36,023 + -2.0
36,023 + -1.0

Or see this region's nucleotide sequence