Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00020 and MMJJ_RS00025 are separated by 10 nucleotides MMJJ_RS00025 and MMJJ_RS00030 are separated by 4 nucleotides
MMJJ_RS00020: MMJJ_RS00020 - 50S ribosomal protein L11 methyltransferase, at 3,668 to 4,555
_RS00020
MMJJ_RS00025: MMJJ_RS00025 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 4,566 to 5,369
_RS00025
MMJJ_RS00030: MMJJ_RS00030 - thymidylate synthase, at 5,374 to 6,249
_RS00030
Position (kb) 4
5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 3.623 kb on + strand at 3.623 kb on + strand at 3.638 kb on - strand at 3.667 kb on - strand at 3.667 kb on - strand at 3.672 kb on - strand at 3.728 kb on + strand at 3.754 kb on - strand at 3.813 kb on - strand, within MMJJ_RS00020 at 3.813 kb on - strand, within MMJJ_RS00020 at 3.823 kb on + strand, within MMJJ_RS00020 at 3.823 kb on + strand, within MMJJ_RS00020 at 3.823 kb on + strand, within MMJJ_RS00020 at 3.823 kb on + strand, within MMJJ_RS00020 at 3.835 kb on + strand, within MMJJ_RS00020 at 3.838 kb on + strand, within MMJJ_RS00020 at 3.937 kb on + strand, within MMJJ_RS00020 at 3.941 kb on - strand, within MMJJ_RS00020 at 3.958 kb on - strand, within MMJJ_RS00020 at 4.031 kb on + strand, within MMJJ_RS00020 at 4.170 kb on - strand, within MMJJ_RS00020 at 4.170 kb on - strand, within MMJJ_RS00020 at 4.170 kb on - strand, within MMJJ_RS00020 at 4.170 kb on - strand, within MMJJ_RS00020 at 4.174 kb on - strand, within MMJJ_RS00020 at 4.174 kb on - strand, within MMJJ_RS00020 at 4.216 kb on - strand, within MMJJ_RS00020 at 4.246 kb on + strand, within MMJJ_RS00020 at 4.347 kb on - strand, within MMJJ_RS00020 at 4.347 kb on - strand, within MMJJ_RS00020 at 4.362 kb on - strand, within MMJJ_RS00020 at 4.363 kb on + strand, within MMJJ_RS00020 at 4.363 kb on + strand, within MMJJ_RS00020 at 4.372 kb on - strand, within MMJJ_RS00020 at 4.372 kb on - strand, within MMJJ_RS00020 at 4.373 kb on + strand, within MMJJ_RS00020 at 4.373 kb on + strand, within MMJJ_RS00020 at 4.373 kb on + strand, within MMJJ_RS00020 at 4.373 kb on + strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.374 kb on - strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.375 kb on + strand, within MMJJ_RS00020 at 4.376 kb on - strand, within MMJJ_RS00020 at 4.376 kb on - strand, within MMJJ_RS00020 at 4.376 kb on - strand, within MMJJ_RS00020 at 4.376 kb on - strand, within MMJJ_RS00020 at 4.376 kb on - strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.377 kb on + strand, within MMJJ_RS00020 at 4.378 kb on - strand, within MMJJ_RS00020 at 4.378 kb on - strand, within MMJJ_RS00020 at 4.379 kb on + strand, within MMJJ_RS00020 at 4.383 kb on - strand, within MMJJ_RS00020 at 4.408 kb on + strand, within MMJJ_RS00020 at 4.408 kb on + strand, within MMJJ_RS00020 at 4.430 kb on + strand, within MMJJ_RS00020 at 4.430 kb on + strand, within MMJJ_RS00020 at 4.483 kb on + strand at 4.550 kb on + strand at 4.661 kb on + strand, within MMJJ_RS00025 at 4.732 kb on - strand, within MMJJ_RS00025 at 4.733 kb on + strand, within MMJJ_RS00025 at 4.733 kb on + strand, within MMJJ_RS00025 at 4.775 kb on + strand, within MMJJ_RS00025 at 4.805 kb on + strand, within MMJJ_RS00025 at 4.867 kb on - strand, within MMJJ_RS00025 at 4.933 kb on - strand, within MMJJ_RS00025 at 4.938 kb on - strand, within MMJJ_RS00025 at 4.939 kb on + strand, within MMJJ_RS00025 at 4.979 kb on - strand, within MMJJ_RS00025 at 4.990 kb on + strand, within MMJJ_RS00025 at 4.990 kb on + strand, within MMJJ_RS00025 at 4.994 kb on - strand, within MMJJ_RS00025 at 4.994 kb on - strand, within MMJJ_RS00025 at 4.999 kb on - strand, within MMJJ_RS00025 at 5.011 kb on - strand, within MMJJ_RS00025 at 5.016 kb on - strand, within MMJJ_RS00025 at 5.017 kb on + strand, within MMJJ_RS00025 at 5.037 kb on + strand, within MMJJ_RS00025 at 5.081 kb on + strand, within MMJJ_RS00025 at 5.081 kb on + strand, within MMJJ_RS00025 at 5.106 kb on + strand, within MMJJ_RS00025 at 5.121 kb on - strand, within MMJJ_RS00025 at 5.122 kb on + strand, within MMJJ_RS00025 at 5.166 kb on + strand, within MMJJ_RS00025 at 5.178 kb on + strand, within MMJJ_RS00025 at 5.183 kb on - strand, within MMJJ_RS00025 at 5.184 kb on + strand, within MMJJ_RS00025 at 5.192 kb on - strand, within MMJJ_RS00025 at 5.285 kb on - strand, within MMJJ_RS00025 at 5.296 kb on - strand at 5.297 kb on + strand at 5.297 kb on + strand at 5.297 kb on + strand at 5.297 kb on + strand at 5.330 kb on + strand at 5.338 kb on - strand at 5.338 kb on - strand at 5.344 kb on + strand at 5.418 kb on + strand at 5.433 kb on + strand at 5.491 kb on - strand, within MMJJ_RS00030 at 5.513 kb on + strand, within MMJJ_RS00030 at 5.572 kb on + strand, within MMJJ_RS00030 at 5.574 kb on - strand, within MMJJ_RS00030 at 5.582 kb on - strand, within MMJJ_RS00030 at 5.582 kb on - strand, within MMJJ_RS00030 at 5.583 kb on + strand, within MMJJ_RS00030 at 5.583 kb on + strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.586 kb on + strand, within MMJJ_RS00030 at 5.586 kb on + strand, within MMJJ_RS00030 at 5.593 kb on + strand, within MMJJ_RS00030 at 5.625 kb on - strand, within MMJJ_RS00030 at 5.652 kb on - strand, within MMJJ_RS00030 at 5.697 kb on + strand, within MMJJ_RS00030 at 5.745 kb on - strand, within MMJJ_RS00030 at 5.745 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.750 kb on + strand, within MMJJ_RS00030 at 5.750 kb on + strand, within MMJJ_RS00030 at 5.796 kb on - strand, within MMJJ_RS00030 at 5.808 kb on - strand, within MMJJ_RS00030 at 5.809 kb on + strand, within MMJJ_RS00030 at 5.809 kb on + strand, within MMJJ_RS00030 at 5.814 kb on - strand, within MMJJ_RS00030 at 5.828 kb on - strand, within MMJJ_RS00030 at 5.828 kb on - strand, within MMJJ_RS00030 at 5.829 kb on + strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 6.005 kb on - strand, within MMJJ_RS00030 at 6.057 kb on + strand, within MMJJ_RS00030 at 6.061 kb on + strand, within MMJJ_RS00030 at 6.105 kb on + strand, within MMJJ_RS00030 at 6.117 kb on - strand, within MMJJ_RS00030 at 6.158 kb on - strand, within MMJJ_RS00030 at 6.158 kb on - strand, within MMJJ_RS00030 at 6.159 kb on + strand, within MMJJ_RS00030 at 6.165 kb on + strand at 6.205 kb on + strand at 6.205 kb on + strand at 6.205 kb on + strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.207 kb on + strand at 6.207 kb on + strand at 6.246 kb on + strand at 6.259 kb on + strand at 6.265 kb on + strand at 6.268 kb on - strand at 6.325 kb on + strand at 6.335 kb on + strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.367 kb on - strand at 6.368 kb on + strand at 6.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 3,623 + +0.6 3,623 + +1.3 3,638 - +1.7 3,667 - +1.0 3,667 - +1.7 3,672 - +0.1 3,728 + -1.6 3,754 - -2.6 3,813 - MMJJ_RS00020 0.16 -0.8 3,813 - MMJJ_RS00020 0.16 +0.4 3,823 + MMJJ_RS00020 0.17 +1.4 3,823 + MMJJ_RS00020 0.17 -1.2 3,823 + MMJJ_RS00020 0.17 +1.5 3,823 + MMJJ_RS00020 0.17 -2.4 3,835 + MMJJ_RS00020 0.19 -1.4 3,838 + MMJJ_RS00020 0.19 +1.2 3,937 + MMJJ_RS00020 0.30 -0.4 3,941 - MMJJ_RS00020 0.31 +0.4 3,958 - MMJJ_RS00020 0.33 +8.4 4,031 + MMJJ_RS00020 0.41 -0.0 4,170 - MMJJ_RS00020 0.57 -1.1 4,170 - MMJJ_RS00020 0.57 -1.1 4,170 - MMJJ_RS00020 0.57 -1.0 4,170 - MMJJ_RS00020 0.57 -0.1 4,174 - MMJJ_RS00020 0.57 -0.8 4,174 - MMJJ_RS00020 0.57 -0.9 4,216 - MMJJ_RS00020 0.62 -0.7 4,246 + MMJJ_RS00020 0.65 -1.7 4,347 - MMJJ_RS00020 0.76 +0.4 4,347 - MMJJ_RS00020 0.76 +1.5 4,362 - MMJJ_RS00020 0.78 -2.3 4,363 + MMJJ_RS00020 0.78 -2.6 4,363 + MMJJ_RS00020 0.78 -0.8 4,372 - MMJJ_RS00020 0.79 -1.2 4,372 - MMJJ_RS00020 0.79 -0.9 4,373 + MMJJ_RS00020 0.79 -1.1 4,373 + MMJJ_RS00020 0.79 +0.9 4,373 + MMJJ_RS00020 0.79 -0.2 4,373 + MMJJ_RS00020 0.79 -1.4 4,374 - MMJJ_RS00020 0.80 -0.6 4,374 - MMJJ_RS00020 0.80 -1.5 4,374 - MMJJ_RS00020 0.80 -0.6 4,374 - MMJJ_RS00020 0.80 +0.5 4,374 - MMJJ_RS00020 0.80 -1.2 4,374 - MMJJ_RS00020 0.80 -1.1 4,374 - MMJJ_RS00020 0.80 -0.4 4,375 + MMJJ_RS00020 0.80 -1.3 4,375 + MMJJ_RS00020 0.80 -1.5 4,375 + MMJJ_RS00020 0.80 +0.1 4,375 + MMJJ_RS00020 0.80 -1.7 4,375 + MMJJ_RS00020 0.80 +2.2 4,375 + MMJJ_RS00020 0.80 -0.4 4,375 + MMJJ_RS00020 0.80 -0.4 4,375 + MMJJ_RS00020 0.80 -0.9 4,375 + MMJJ_RS00020 0.80 +0.0 4,376 - MMJJ_RS00020 0.80 -1.7 4,376 - MMJJ_RS00020 0.80 +0.2 4,376 - MMJJ_RS00020 0.80 -1.9 4,376 - MMJJ_RS00020 0.80 -1.1 4,376 - MMJJ_RS00020 0.80 -1.5 4,377 + MMJJ_RS00020 0.80 -1.2 4,377 + MMJJ_RS00020 0.80 +1.4 4,377 + MMJJ_RS00020 0.80 +0.4 4,377 + MMJJ_RS00020 0.80 +1.1 4,377 + MMJJ_RS00020 0.80 -1.7 4,377 + MMJJ_RS00020 0.80 -0.5 4,377 + MMJJ_RS00020 0.80 -0.4 4,377 + MMJJ_RS00020 0.80 +0.5 4,378 - MMJJ_RS00020 0.80 +0.8 4,378 - MMJJ_RS00020 0.80 -2.3 4,379 + MMJJ_RS00020 0.80 -1.6 4,383 - MMJJ_RS00020 0.81 -0.2 4,408 + MMJJ_RS00020 0.83 +1.1 4,408 + MMJJ_RS00020 0.83 -0.2 4,430 + MMJJ_RS00020 0.86 +0.8 4,430 + MMJJ_RS00020 0.86 +0.2 4,483 + +0.5 4,550 + +0.9 4,661 + MMJJ_RS00025 0.12 +0.6 4,732 - MMJJ_RS00025 0.21 +0.1 4,733 + MMJJ_RS00025 0.21 -1.3 4,733 + MMJJ_RS00025 0.21 +3.4 4,775 + MMJJ_RS00025 0.26 -0.9 4,805 + MMJJ_RS00025 0.30 -1.7 4,867 - MMJJ_RS00025 0.37 -2.9 4,933 - MMJJ_RS00025 0.46 -2.5 4,938 - MMJJ_RS00025 0.46 -1.9 4,939 + MMJJ_RS00025 0.46 -0.9 4,979 - MMJJ_RS00025 0.51 -0.6 4,990 + MMJJ_RS00025 0.53 +0.3 4,990 + MMJJ_RS00025 0.53 -1.5 4,994 - MMJJ_RS00025 0.53 -0.5 4,994 - MMJJ_RS00025 0.53 -2.0 4,999 - MMJJ_RS00025 0.54 +0.6 5,011 - MMJJ_RS00025 0.55 -0.9 5,016 - MMJJ_RS00025 0.56 -1.0 5,017 + MMJJ_RS00025 0.56 -2.5 5,037 + MMJJ_RS00025 0.59 -0.1 5,081 + MMJJ_RS00025 0.64 -0.9 5,081 + MMJJ_RS00025 0.64 -3.9 5,106 + MMJJ_RS00025 0.67 -2.9 5,121 - MMJJ_RS00025 0.69 -0.9 5,122 + MMJJ_RS00025 0.69 -2.1 5,166 + MMJJ_RS00025 0.75 -2.4 5,178 + MMJJ_RS00025 0.76 -1.4 5,183 - MMJJ_RS00025 0.77 +1.4 5,184 + MMJJ_RS00025 0.77 -0.6 5,192 - MMJJ_RS00025 0.78 -0.9 5,285 - MMJJ_RS00025 0.89 -1.5 5,296 - -1.4 5,297 + +0.7 5,297 + -1.7 5,297 + -0.7 5,297 + -1.5 5,330 + -1.2 5,338 - -1.0 5,338 - -2.7 5,344 + +2.9 5,418 + -0.0 5,433 + -0.6 5,491 - MMJJ_RS00030 0.13 -0.7 5,513 + MMJJ_RS00030 0.16 +0.1 5,572 + MMJJ_RS00030 0.23 -0.6 5,574 - MMJJ_RS00030 0.23 +1.2 5,582 - MMJJ_RS00030 0.24 +0.3 5,582 - MMJJ_RS00030 0.24 -1.8 5,583 + MMJJ_RS00030 0.24 -0.9 5,583 + MMJJ_RS00030 0.24 -2.3 5,585 - MMJJ_RS00030 0.24 -0.5 5,585 - MMJJ_RS00030 0.24 -0.3 5,585 - MMJJ_RS00030 0.24 +0.4 5,586 + MMJJ_RS00030 0.24 +0.0 5,586 + MMJJ_RS00030 0.24 +0.8 5,593 + MMJJ_RS00030 0.25 -1.5 5,625 - MMJJ_RS00030 0.29 +2.7 5,652 - MMJJ_RS00030 0.32 -0.1 5,697 + MMJJ_RS00030 0.37 -1.0 5,745 - MMJJ_RS00030 0.42 +2.0 5,745 - MMJJ_RS00030 0.42 -1.1 5,749 - MMJJ_RS00030 0.43 -0.4 5,749 - MMJJ_RS00030 0.43 -0.1 5,749 - MMJJ_RS00030 0.43 +1.4 5,750 + MMJJ_RS00030 0.43 +1.4 5,750 + MMJJ_RS00030 0.43 -0.4 5,796 - MMJJ_RS00030 0.48 -0.3 5,808 - MMJJ_RS00030 0.50 -1.6 5,809 + MMJJ_RS00030 0.50 +1.5 5,809 + MMJJ_RS00030 0.50 -0.3 5,814 - MMJJ_RS00030 0.50 +1.4 5,828 - MMJJ_RS00030 0.52 -1.4 5,828 - MMJJ_RS00030 0.52 +0.6 5,829 + MMJJ_RS00030 0.52 +0.8 5,860 - MMJJ_RS00030 0.55 -1.6 5,860 - MMJJ_RS00030 0.55 -2.5 5,860 - MMJJ_RS00030 0.55 -0.1 6,005 - MMJJ_RS00030 0.72 +1.1 6,057 + MMJJ_RS00030 0.78 +1.2 6,061 + MMJJ_RS00030 0.78 -0.4 6,105 + MMJJ_RS00030 0.83 -0.6 6,117 - MMJJ_RS00030 0.85 -0.9 6,158 - MMJJ_RS00030 0.89 +1.4 6,158 - MMJJ_RS00030 0.89 +0.2 6,159 + MMJJ_RS00030 0.90 +0.5 6,165 + +0.1 6,205 + -0.2 6,205 + +0.3 6,205 + -0.4 6,206 - -1.4 6,206 - -1.2 6,206 - +2.1 6,206 - +1.1 6,207 + -1.3 6,207 + +0.1 6,246 + -1.0 6,259 + -0.6 6,265 + -0.5 6,268 - +1.8 6,325 + +0.0 6,335 + -1.3 6,365 - -1.2 6,365 - -3.1 6,365 - -1.3 6,365 - -1.5 6,366 + +0.3 6,366 + -0.0 6,366 + -1.8 6,366 + -0.6 6,366 + -2.6 6,367 - -1.5 6,368 + -1.0 6,369 - -1.1
Or see this region's nucleotide sequence