Experiment: Lactulose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14410 and CA265_RS14415 are separated by 3 nucleotides CA265_RS14415 and CA265_RS14420 are separated by 103 nucleotides
CA265_RS14410: CA265_RS14410 - 4-amino-4-deoxychorismate lyase, at 3,634,779 to 3,635,618
_RS14410
CA265_RS14415: CA265_RS14415 - pseudouridine synthase, at 3,635,622 to 3,636,650
_RS14415
CA265_RS14420: CA265_RS14420 - hypothetical protein, at 3,636,754 to 3,637,782
_RS14420
Position (kb)
3635
3636
3637 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3634.632 kb on + strand at 3634.632 kb on + strand at 3634.702 kb on + strand at 3634.702 kb on + strand at 3634.703 kb on - strand at 3634.775 kb on - strand at 3634.775 kb on - strand at 3634.782 kb on + strand at 3634.782 kb on + strand at 3634.782 kb on + strand at 3634.828 kb on + strand at 3634.909 kb on - strand, within CA265_RS14410 at 3634.920 kb on - strand, within CA265_RS14410 at 3634.955 kb on + strand, within CA265_RS14410 at 3634.984 kb on - strand, within CA265_RS14410 at 3635.020 kb on + strand, within CA265_RS14410 at 3635.021 kb on - strand, within CA265_RS14410 at 3635.036 kb on - strand, within CA265_RS14410 at 3635.143 kb on + strand, within CA265_RS14410 at 3635.220 kb on + strand, within CA265_RS14410 at 3635.221 kb on - strand, within CA265_RS14410 at 3635.242 kb on + strand, within CA265_RS14410 at 3635.242 kb on + strand, within CA265_RS14410 at 3635.243 kb on - strand, within CA265_RS14410 at 3635.247 kb on - strand, within CA265_RS14410 at 3635.279 kb on - strand, within CA265_RS14410 at 3635.463 kb on - strand, within CA265_RS14410 at 3635.472 kb on + strand, within CA265_RS14410 at 3635.472 kb on + strand, within CA265_RS14410 at 3635.473 kb on - strand, within CA265_RS14410 at 3635.493 kb on - strand, within CA265_RS14410 at 3635.493 kb on - strand, within CA265_RS14410 at 3635.493 kb on - strand, within CA265_RS14410 at 3635.495 kb on + strand, within CA265_RS14410 at 3635.511 kb on - strand, within CA265_RS14410 at 3635.574 kb on + strand at 3635.599 kb on - strand at 3635.605 kb on + strand at 3635.623 kb on + strand at 3635.624 kb on - strand at 3635.630 kb on + strand at 3635.631 kb on - strand at 3635.699 kb on + strand at 3635.699 kb on + strand at 3635.700 kb on - strand at 3635.702 kb on - strand at 3635.723 kb on + strand at 3635.777 kb on + strand, within CA265_RS14415 at 3635.778 kb on - strand, within CA265_RS14415 at 3635.778 kb on - strand, within CA265_RS14415 at 3635.826 kb on - strand, within CA265_RS14415 at 3635.836 kb on + strand, within CA265_RS14415 at 3635.930 kb on + strand, within CA265_RS14415 at 3635.953 kb on - strand, within CA265_RS14415 at 3635.958 kb on - strand, within CA265_RS14415 at 3635.959 kb on + strand, within CA265_RS14415 at 3635.959 kb on + strand, within CA265_RS14415 at 3636.041 kb on - strand, within CA265_RS14415 at 3636.089 kb on + strand, within CA265_RS14415 at 3636.090 kb on - strand, within CA265_RS14415 at 3636.090 kb on - strand, within CA265_RS14415 at 3636.091 kb on + strand, within CA265_RS14415 at 3636.091 kb on + strand, within CA265_RS14415 at 3636.186 kb on - strand, within CA265_RS14415 at 3636.266 kb on + strand, within CA265_RS14415 at 3636.283 kb on + strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.328 kb on - strand, within CA265_RS14415 at 3636.360 kb on - strand, within CA265_RS14415 at 3636.370 kb on - strand, within CA265_RS14415 at 3636.434 kb on - strand, within CA265_RS14415 at 3636.882 kb on - strand, within CA265_RS14420 at 3636.889 kb on + strand, within CA265_RS14420 at 3637.035 kb on - strand, within CA265_RS14420 at 3637.050 kb on - strand, within CA265_RS14420 at 3637.059 kb on + strand, within CA265_RS14420 at 3637.059 kb on + strand, within CA265_RS14420 at 3637.155 kb on + strand, within CA265_RS14420 at 3637.187 kb on + strand, within CA265_RS14420 at 3637.235 kb on + strand, within CA265_RS14420 at 3637.240 kb on - strand, within CA265_RS14420 at 3637.254 kb on + strand, within CA265_RS14420 at 3637.304 kb on + strand, within CA265_RS14420 at 3637.305 kb on - strand, within CA265_RS14420 at 3637.362 kb on + strand, within CA265_RS14420 at 3637.395 kb on - strand, within CA265_RS14420 at 3637.421 kb on - strand, within CA265_RS14420 at 3637.443 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.625 kb on - strand, within CA265_RS14420
Per-strain Table
Position Strand Gene LocusTag Fraction Lactulose 20mM (C) remove 3,634,632 + +0.5 3,634,632 + -1.0 3,634,702 + +0.4 3,634,702 + -1.6 3,634,703 - -2.0 3,634,775 - -2.8 3,634,775 - +1.6 3,634,782 + -3.3 3,634,782 + -2.7 3,634,782 + +0.2 3,634,828 + -3.5 3,634,909 - CA265_RS14410 0.15 +0.6 3,634,920 - CA265_RS14410 0.17 +0.9 3,634,955 + CA265_RS14410 0.21 -0.3 3,634,984 - CA265_RS14410 0.24 -2.3 3,635,020 + CA265_RS14410 0.29 +1.2 3,635,021 - CA265_RS14410 0.29 -0.5 3,635,036 - CA265_RS14410 0.31 -2.3 3,635,143 + CA265_RS14410 0.43 +1.3 3,635,220 + CA265_RS14410 0.53 +1.3 3,635,221 - CA265_RS14410 0.53 +3.6 3,635,242 + CA265_RS14410 0.55 +0.5 3,635,242 + CA265_RS14410 0.55 -1.4 3,635,243 - CA265_RS14410 0.55 +0.3 3,635,247 - CA265_RS14410 0.56 -2.7 3,635,279 - CA265_RS14410 0.60 -4.8 3,635,463 - CA265_RS14410 0.81 -2.7 3,635,472 + CA265_RS14410 0.82 -3.5 3,635,472 + CA265_RS14410 0.82 +1.5 3,635,473 - CA265_RS14410 0.83 +0.5 3,635,493 - CA265_RS14410 0.85 -4.9 3,635,493 - CA265_RS14410 0.85 +0.1 3,635,493 - CA265_RS14410 0.85 +1.0 3,635,495 + CA265_RS14410 0.85 +2.2 3,635,511 - CA265_RS14410 0.87 +1.0 3,635,574 + -2.2 3,635,599 - -0.4 3,635,605 + +1.8 3,635,623 + -0.0 3,635,624 - +1.9 3,635,630 + +0.5 3,635,631 - +0.2 3,635,699 + -0.2 3,635,699 + +1.4 3,635,700 - +1.3 3,635,702 - +1.3 3,635,723 + -1.2 3,635,777 + CA265_RS14415 0.15 -1.8 3,635,778 - CA265_RS14415 0.15 -1.5 3,635,778 - CA265_RS14415 0.15 +3.3 3,635,826 - CA265_RS14415 0.20 +3.4 3,635,836 + CA265_RS14415 0.21 +2.9 3,635,930 + CA265_RS14415 0.30 +1.3 3,635,953 - CA265_RS14415 0.32 +0.4 3,635,958 - CA265_RS14415 0.33 +0.8 3,635,959 + CA265_RS14415 0.33 -2.4 3,635,959 + CA265_RS14415 0.33 +4.5 3,636,041 - CA265_RS14415 0.41 -0.9 3,636,089 + CA265_RS14415 0.45 -1.5 3,636,090 - CA265_RS14415 0.45 -1.4 3,636,090 - CA265_RS14415 0.45 +4.0 3,636,091 + CA265_RS14415 0.46 -0.2 3,636,091 + CA265_RS14415 0.46 +2.7 3,636,186 - CA265_RS14415 0.55 -1.9 3,636,266 + CA265_RS14415 0.63 -2.1 3,636,283 + CA265_RS14415 0.64 +1.5 3,636,304 - CA265_RS14415 0.66 +3.4 3,636,304 - CA265_RS14415 0.66 -0.4 3,636,304 - CA265_RS14415 0.66 -0.9 3,636,328 - CA265_RS14415 0.69 +2.5 3,636,360 - CA265_RS14415 0.72 -2.3 3,636,370 - CA265_RS14415 0.73 -2.3 3,636,434 - CA265_RS14415 0.79 -0.8 3,636,882 - CA265_RS14420 0.12 -0.8 3,636,889 + CA265_RS14420 0.13 -0.0 3,637,035 - CA265_RS14420 0.27 +1.3 3,637,050 - CA265_RS14420 0.29 -0.4 3,637,059 + CA265_RS14420 0.30 -0.8 3,637,059 + CA265_RS14420 0.30 +0.6 3,637,155 + CA265_RS14420 0.39 -3.2 3,637,187 + CA265_RS14420 0.42 +0.1 3,637,235 + CA265_RS14420 0.47 -0.8 3,637,240 - CA265_RS14420 0.47 -2.0 3,637,254 + CA265_RS14420 0.49 +0.2 3,637,304 + CA265_RS14420 0.53 -0.2 3,637,305 - CA265_RS14420 0.54 +1.9 3,637,362 + CA265_RS14420 0.59 -0.5 3,637,395 - CA265_RS14420 0.62 +0.5 3,637,421 - CA265_RS14420 0.65 -1.8 3,637,443 + CA265_RS14420 0.67 -1.5 3,637,624 + CA265_RS14420 0.85 -1.7 3,637,624 + CA265_RS14420 0.85 +1.0 3,637,624 + CA265_RS14420 0.85 +0.1 3,637,625 - CA265_RS14420 0.85 +1.6
Or see this region's nucleotide sequence