Experiment: Lactulose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09835 and CA265_RS09840 are separated by 76 nucleotides CA265_RS09840 and CA265_RS09845 are separated by 179 nucleotides CA265_RS09845 and CA265_RS09850 are separated by 58 nucleotides CA265_RS09850 and CA265_RS09855 are separated by 223 nucleotides
CA265_RS09835: CA265_RS09835 - hypothetical protein, at 2,426,690 to 2,427,319
_RS09835
CA265_RS09840: CA265_RS09840 - YajQ family cyclic di-GMP-binding protein, at 2,427,396 to 2,427,887
_RS09840
CA265_RS09845: CA265_RS09845 - GNAT family N-acetyltransferase, at 2,428,067 to 2,428,513
_RS09845
CA265_RS09850: CA265_RS09850 - hypothetical protein, at 2,428,572 to 2,429,168
_RS09850
CA265_RS09855: CA265_RS09855 - peptidase S8, at 2,429,392 to 2,431,005
_RS09855
Position (kb)
2428
2429 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2427.231 kb on - strand, within CA265_RS09835 at 2427.231 kb on - strand, within CA265_RS09835 at 2427.419 kb on + strand at 2427.439 kb on + strand at 2427.561 kb on - strand, within CA265_RS09840 at 2427.690 kb on - strand, within CA265_RS09840 at 2427.690 kb on - strand, within CA265_RS09840 at 2427.823 kb on + strand, within CA265_RS09840 at 2427.824 kb on - strand, within CA265_RS09840 at 2427.867 kb on + strand at 2427.869 kb on + strand at 2427.869 kb on + strand at 2427.882 kb on - strand at 2427.886 kb on - strand at 2427.893 kb on - strand at 2427.938 kb on - strand at 2427.938 kb on - strand at 2427.951 kb on + strand at 2428.009 kb on + strand at 2428.010 kb on - strand at 2428.116 kb on + strand, within CA265_RS09845 at 2428.131 kb on + strand, within CA265_RS09845 at 2428.132 kb on - strand, within CA265_RS09845 at 2428.132 kb on - strand, within CA265_RS09845 at 2428.132 kb on - strand, within CA265_RS09845 at 2428.139 kb on + strand, within CA265_RS09845 at 2428.140 kb on - strand, within CA265_RS09845 at 2428.143 kb on + strand, within CA265_RS09845 at 2428.149 kb on + strand, within CA265_RS09845 at 2428.150 kb on - strand, within CA265_RS09845 at 2428.172 kb on - strand, within CA265_RS09845 at 2428.189 kb on - strand, within CA265_RS09845 at 2428.307 kb on - strand, within CA265_RS09845 at 2428.408 kb on - strand, within CA265_RS09845 at 2428.424 kb on + strand, within CA265_RS09845 at 2428.425 kb on - strand, within CA265_RS09845 at 2428.474 kb on + strand at 2428.475 kb on - strand at 2428.475 kb on - strand at 2428.491 kb on + strand at 2428.493 kb on + strand at 2428.493 kb on + strand at 2428.553 kb on + strand at 2428.582 kb on + strand at 2428.683 kb on - strand, within CA265_RS09850 at 2428.709 kb on + strand, within CA265_RS09850 at 2428.921 kb on + strand, within CA265_RS09850 at 2428.925 kb on + strand, within CA265_RS09850 at 2429.007 kb on + strand, within CA265_RS09850 at 2429.007 kb on + strand, within CA265_RS09850 at 2429.044 kb on + strand, within CA265_RS09850 at 2429.088 kb on - strand, within CA265_RS09850 at 2429.102 kb on + strand, within CA265_RS09850 at 2429.106 kb on - strand, within CA265_RS09850 at 2429.152 kb on + strand at 2429.221 kb on + strand at 2429.221 kb on + strand at 2429.251 kb on + strand at 2429.252 kb on - strand at 2429.284 kb on + strand at 2429.385 kb on + strand at 2429.385 kb on + strand at 2429.397 kb on + strand at 2429.397 kb on + strand at 2429.423 kb on + strand at 2429.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactulose 20mM (C) remove 2,427,231 - CA265_RS09835 0.86 +2.0 2,427,231 - CA265_RS09835 0.86 +0.7 2,427,419 + -1.5 2,427,439 + -0.8 2,427,561 - CA265_RS09840 0.34 +0.2 2,427,690 - CA265_RS09840 0.60 -1.1 2,427,690 - CA265_RS09840 0.60 +0.4 2,427,823 + CA265_RS09840 0.87 +0.0 2,427,824 - CA265_RS09840 0.87 -1.7 2,427,867 + +1.2 2,427,869 + +0.8 2,427,869 + -1.6 2,427,882 - +0.7 2,427,886 - +0.2 2,427,893 - -3.2 2,427,938 - +0.3 2,427,938 - +0.2 2,427,951 + -0.5 2,428,009 + -1.5 2,428,010 - -1.8 2,428,116 + CA265_RS09845 0.11 +3.2 2,428,131 + CA265_RS09845 0.14 +1.0 2,428,132 - CA265_RS09845 0.15 -4.1 2,428,132 - CA265_RS09845 0.15 -1.0 2,428,132 - CA265_RS09845 0.15 -0.5 2,428,139 + CA265_RS09845 0.16 +0.2 2,428,140 - CA265_RS09845 0.16 -3.5 2,428,143 + CA265_RS09845 0.17 -3.0 2,428,149 + CA265_RS09845 0.18 -3.8 2,428,150 - CA265_RS09845 0.19 -3.3 2,428,172 - CA265_RS09845 0.23 -0.3 2,428,189 - CA265_RS09845 0.27 -0.4 2,428,307 - CA265_RS09845 0.54 +0.2 2,428,408 - CA265_RS09845 0.76 +0.3 2,428,424 + CA265_RS09845 0.80 +2.4 2,428,425 - CA265_RS09845 0.80 -3.8 2,428,474 + -0.4 2,428,475 - -0.6 2,428,475 - -0.5 2,428,491 + +1.0 2,428,493 + +0.1 2,428,493 + -0.3 2,428,553 + -1.0 2,428,582 + +0.5 2,428,683 - CA265_RS09850 0.19 +0.4 2,428,709 + CA265_RS09850 0.23 +0.8 2,428,921 + CA265_RS09850 0.58 +0.9 2,428,925 + CA265_RS09850 0.59 +1.0 2,429,007 + CA265_RS09850 0.73 -3.6 2,429,007 + CA265_RS09850 0.73 -0.1 2,429,044 + CA265_RS09850 0.79 -1.3 2,429,088 - CA265_RS09850 0.86 +0.8 2,429,102 + CA265_RS09850 0.89 +1.6 2,429,106 - CA265_RS09850 0.89 +2.0 2,429,152 + -1.1 2,429,221 + -0.9 2,429,221 + -2.0 2,429,251 + -0.6 2,429,252 - +0.2 2,429,284 + +0.3 2,429,385 + +0.7 2,429,385 + -2.4 2,429,397 + +1.5 2,429,397 + +1.0 2,429,423 + +0.0 2,429,509 - -0.7
Or see this region's nucleotide sequence