Experiment: Lactulose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09005 and CA265_RS09010 are separated by 79 nucleotides CA265_RS09010 and CA265_RS09015 are separated by 233 nucleotides CA265_RS09015 and CA265_RS09020 are separated by 168 nucleotides
CA265_RS09005: CA265_RS09005 - phosphoserine transaminase, at 2,246,240 to 2,247,313
_RS09005
CA265_RS09010: CA265_RS09010 - 3-phosphoglycerate dehydrogenase, at 2,247,393 to 2,248,340
_RS09010
CA265_RS09015: CA265_RS09015 - hypothetical protein, at 2,248,574 to 2,249,098
_RS09015
CA265_RS09020: CA265_RS09020 - hypothetical protein, at 2,249,267 to 2,250,046
_RS09020
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2247.347 kb on - strand at 2247.386 kb on + strand at 2247.587 kb on - strand, within CA265_RS09010 at 2247.617 kb on + strand, within CA265_RS09010 at 2247.630 kb on + strand, within CA265_RS09010 at 2247.713 kb on - strand, within CA265_RS09010 at 2247.799 kb on - strand, within CA265_RS09010 at 2247.836 kb on + strand, within CA265_RS09010 at 2247.867 kb on - strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.917 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.030 kb on + strand, within CA265_RS09010 at 2248.100 kb on + strand, within CA265_RS09010 at 2248.101 kb on - strand, within CA265_RS09010 at 2248.156 kb on + strand, within CA265_RS09010 at 2248.259 kb on + strand at 2248.260 kb on - strand at 2248.260 kb on - strand at 2248.341 kb on + strand at 2248.360 kb on + strand at 2248.528 kb on + strand at 2248.582 kb on + strand at 2248.583 kb on - strand at 2248.583 kb on - strand at 2248.607 kb on + strand at 2248.666 kb on + strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.754 kb on - strand, within CA265_RS09015 at 2248.755 kb on + strand, within CA265_RS09015 at 2248.756 kb on - strand, within CA265_RS09015 at 2248.780 kb on + strand, within CA265_RS09015 at 2248.819 kb on + strand, within CA265_RS09015 at 2248.822 kb on - strand, within CA265_RS09015 at 2248.866 kb on - strand, within CA265_RS09015 at 2248.959 kb on + strand, within CA265_RS09015 at 2249.005 kb on + strand, within CA265_RS09015 at 2249.021 kb on - strand, within CA265_RS09015 at 2249.022 kb on + strand, within CA265_RS09015 at 2249.033 kb on - strand, within CA265_RS09015 at 2249.056 kb on + strand at 2249.056 kb on + strand at 2249.066 kb on + strand at 2249.071 kb on + strand at 2249.082 kb on + strand at 2249.143 kb on + strand at 2249.166 kb on - strand at 2249.213 kb on - strand at 2249.222 kb on + strand at 2249.235 kb on + strand at 2249.268 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactulose 20mM (C) remove 2,247,347 - -2.6 2,247,386 + -2.6 2,247,587 - CA265_RS09010 0.20 -2.1 2,247,617 + CA265_RS09010 0.24 -0.4 2,247,630 + CA265_RS09010 0.25 -1.7 2,247,713 - CA265_RS09010 0.34 -2.6 2,247,799 - CA265_RS09010 0.43 -3.6 2,247,836 + CA265_RS09010 0.47 -0.8 2,247,867 - CA265_RS09010 0.50 -2.4 2,247,916 + CA265_RS09010 0.55 +0.2 2,247,916 + CA265_RS09010 0.55 -3.2 2,247,916 + CA265_RS09010 0.55 -2.1 2,247,917 - CA265_RS09010 0.55 -0.1 2,248,008 - CA265_RS09010 0.65 -2.1 2,248,008 - CA265_RS09010 0.65 -2.9 2,248,030 + CA265_RS09010 0.67 -4.3 2,248,100 + CA265_RS09010 0.75 -4.2 2,248,101 - CA265_RS09010 0.75 -1.8 2,248,156 + CA265_RS09010 0.80 -0.8 2,248,259 + +0.6 2,248,260 - -3.8 2,248,260 - -1.6 2,248,341 + -1.6 2,248,360 + +0.4 2,248,528 + +0.3 2,248,582 + -0.2 2,248,583 - +0.9 2,248,583 - -0.4 2,248,607 + +1.0 2,248,666 + CA265_RS09015 0.18 +1.5 2,248,669 - CA265_RS09015 0.18 -0.1 2,248,669 - CA265_RS09015 0.18 -2.8 2,248,669 - CA265_RS09015 0.18 +0.6 2,248,754 - CA265_RS09015 0.34 -0.8 2,248,755 + CA265_RS09015 0.34 +0.3 2,248,756 - CA265_RS09015 0.35 +0.1 2,248,780 + CA265_RS09015 0.39 +1.3 2,248,819 + CA265_RS09015 0.47 +0.2 2,248,822 - CA265_RS09015 0.47 -0.5 2,248,866 - CA265_RS09015 0.56 -2.1 2,248,959 + CA265_RS09015 0.73 +1.3 2,249,005 + CA265_RS09015 0.82 +0.0 2,249,021 - CA265_RS09015 0.85 -0.7 2,249,022 + CA265_RS09015 0.85 +1.5 2,249,033 - CA265_RS09015 0.87 +1.0 2,249,056 + -1.8 2,249,056 + +2.6 2,249,066 + -3.3 2,249,071 + +0.6 2,249,082 + +2.1 2,249,143 + -0.1 2,249,166 - +0.4 2,249,213 - +1.3 2,249,222 + +0.8 2,249,235 + +1.2 2,249,268 + +0.7
Or see this region's nucleotide sequence