Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31120 and AZOBR_RS31125 are separated by 70 nucleotides AZOBR_RS31125 and AZOBR_RS31130 are separated by 79 nucleotides
AZOBR_RS31120: AZOBR_RS31120 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 345,819 to 347,123
_RS31120
AZOBR_RS31125: AZOBR_RS31125 - biotin synthase, at 347,194 to 348,117
_RS31125
AZOBR_RS31130: AZOBR_RS31130 - membrane protein, at 348,197 to 348,904
_RS31130
Position (kb) 347
348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 346.248 kb on + strand, within AZOBR_RS31120 at 346.249 kb on - strand, within AZOBR_RS31120 at 346.249 kb on - strand, within AZOBR_RS31120 at 346.300 kb on - strand, within AZOBR_RS31120 at 346.344 kb on + strand, within AZOBR_RS31120 at 346.956 kb on + strand, within AZOBR_RS31120 at 347.035 kb on + strand at 347.035 kb on + strand at 347.036 kb on - strand at 347.037 kb on + strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.038 kb on - strand at 347.085 kb on + strand at 347.383 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.632 kb on + strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.633 kb on - strand, within AZOBR_RS31125 at 347.635 kb on + strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.636 kb on - strand, within AZOBR_RS31125 at 347.668 kb on + strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.669 kb on - strand, within AZOBR_RS31125 at 347.959 kb on + strand, within AZOBR_RS31125 at 348.043 kb on + strand at 348.044 kb on - strand at 348.044 kb on - strand at 348.233 kb on + strand at 348.233 kb on + strand at 348.234 kb on - strand at 348.234 kb on - strand at 348.234 kb on - strand at 348.269 kb on + strand, within AZOBR_RS31130 at 348.270 kb on - strand, within AZOBR_RS31130 at 348.270 kb on - strand, within AZOBR_RS31130 at 348.434 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.575 kb on + strand, within AZOBR_RS31130 at 348.602 kb on + strand, within AZOBR_RS31130 at 348.603 kb on - strand, within AZOBR_RS31130 at 348.603 kb on - strand, within AZOBR_RS31130 at 348.638 kb on + strand, within AZOBR_RS31130 at 348.639 kb on - strand, within AZOBR_RS31130 at 348.639 kb on - strand, within AZOBR_RS31130 at 348.671 kb on + strand, within AZOBR_RS31130 at 348.671 kb on + strand, within AZOBR_RS31130 at 348.672 kb on - strand, within AZOBR_RS31130 at 348.672 kb on - strand, within AZOBR_RS31130 at 348.987 kb on - strand at 348.987 kb on - strand at 348.987 kb on - strand at 349.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 346,248 + AZOBR_RS31120 0.33 +0.7 346,249 - AZOBR_RS31120 0.33 -3.0 346,249 - AZOBR_RS31120 0.33 +0.6 346,300 - AZOBR_RS31120 0.37 -1.5 346,344 + AZOBR_RS31120 0.40 +0.3 346,956 + AZOBR_RS31120 0.87 -0.5 347,035 + -0.9 347,035 + +0.8 347,036 - -0.7 347,037 + -0.8 347,038 - -0.2 347,038 - +0.7 347,038 - -0.5 347,038 - +0.3 347,038 - +1.3 347,085 + +2.3 347,383 + AZOBR_RS31125 0.20 -2.6 347,632 + AZOBR_RS31125 0.47 -1.1 347,632 + AZOBR_RS31125 0.47 -1.9 347,633 - AZOBR_RS31125 0.48 -0.4 347,633 - AZOBR_RS31125 0.48 -1.1 347,635 + AZOBR_RS31125 0.48 -0.8 347,636 - AZOBR_RS31125 0.48 +0.2 347,636 - AZOBR_RS31125 0.48 +1.0 347,636 - AZOBR_RS31125 0.48 -1.5 347,636 - AZOBR_RS31125 0.48 +0.8 347,636 - AZOBR_RS31125 0.48 +2.7 347,636 - AZOBR_RS31125 0.48 +2.1 347,668 + AZOBR_RS31125 0.51 -1.6 347,669 - AZOBR_RS31125 0.51 +1.3 347,669 - AZOBR_RS31125 0.51 +0.8 347,959 + AZOBR_RS31125 0.83 +0.3 348,043 + +0.7 348,044 - -2.5 348,044 - +0.1 348,233 + -0.4 348,233 + -0.1 348,234 - +0.4 348,234 - +0.4 348,234 - +0.1 348,269 + AZOBR_RS31130 0.10 +0.9 348,270 - AZOBR_RS31130 0.10 -0.9 348,270 - AZOBR_RS31130 0.10 -2.1 348,434 + AZOBR_RS31130 0.33 -1.9 348,575 + AZOBR_RS31130 0.53 +0.8 348,575 + AZOBR_RS31130 0.53 +1.4 348,575 + AZOBR_RS31130 0.53 -0.1 348,602 + AZOBR_RS31130 0.57 +1.1 348,603 - AZOBR_RS31130 0.57 +0.4 348,603 - AZOBR_RS31130 0.57 +0.1 348,638 + AZOBR_RS31130 0.62 +1.9 348,639 - AZOBR_RS31130 0.62 +0.4 348,639 - AZOBR_RS31130 0.62 -1.7 348,671 + AZOBR_RS31130 0.67 +2.4 348,671 + AZOBR_RS31130 0.67 +1.8 348,672 - AZOBR_RS31130 0.67 -3.2 348,672 - AZOBR_RS31130 0.67 +1.0 348,987 - +0.8 348,987 - -1.2 348,987 - -0.2 349,017 - -1.5
Or see this region's nucleotide sequence