Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30220 and AZOBR_RS30225 are separated by 30 nucleotides AZOBR_RS30225 and AZOBR_RS30230 are separated by 68 nucleotides AZOBR_RS30230 and AZOBR_RS30235 are separated by 269 nucleotides
AZOBR_RS30220: AZOBR_RS30220 - 3-ketoacyl-ACP reductase, at 126,751 to 127,515
_RS30220
AZOBR_RS30225: AZOBR_RS30225 - 5-keto-4-deoxyuronate isomerase, at 127,546 to 128,382
_RS30225
AZOBR_RS30230: AZOBR_RS30230 - GntR family transcriptional regulator, at 128,451 to 129,152
_RS30230
AZOBR_RS30235: AZOBR_RS30235 - 2-keto-3-deoxygluconate permease, at 129,422 to 130,375
_RS30235
Position (kb)
128
129
130 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 127.521 kb on + strand at 127.522 kb on - strand at 127.563 kb on + strand at 127.625 kb on + strand at 127.625 kb on + strand at 127.626 kb on - strand at 127.626 kb on - strand at 127.763 kb on + strand, within AZOBR_RS30225 at 127.769 kb on + strand, within AZOBR_RS30225 at 127.769 kb on + strand, within AZOBR_RS30225 at 127.769 kb on + strand, within AZOBR_RS30225 at 127.769 kb on + strand, within AZOBR_RS30225 at 127.770 kb on - strand, within AZOBR_RS30225 at 127.770 kb on - strand, within AZOBR_RS30225 at 127.770 kb on - strand, within AZOBR_RS30225 at 127.770 kb on - strand, within AZOBR_RS30225 at 127.892 kb on + strand, within AZOBR_RS30225 at 127.892 kb on + strand, within AZOBR_RS30225 at 127.892 kb on + strand, within AZOBR_RS30225 at 127.893 kb on - strand, within AZOBR_RS30225 at 127.893 kb on - strand, within AZOBR_RS30225 at 127.893 kb on - strand, within AZOBR_RS30225 at 128.003 kb on + strand, within AZOBR_RS30225 at 128.003 kb on + strand, within AZOBR_RS30225 at 128.004 kb on - strand, within AZOBR_RS30225 at 128.069 kb on + strand, within AZOBR_RS30225 at 128.070 kb on - strand, within AZOBR_RS30225 at 128.070 kb on - strand, within AZOBR_RS30225 at 128.070 kb on - strand, within AZOBR_RS30225 at 128.070 kb on - strand, within AZOBR_RS30225 at 128.261 kb on + strand, within AZOBR_RS30225 at 128.261 kb on + strand, within AZOBR_RS30225 at 128.262 kb on - strand, within AZOBR_RS30225 at 128.262 kb on - strand, within AZOBR_RS30225 at 128.333 kb on + strand at 128.334 kb on - strand at 128.693 kb on - strand, within AZOBR_RS30230 at 128.840 kb on - strand, within AZOBR_RS30230 at 129.135 kb on + strand at 129.135 kb on + strand at 129.135 kb on + strand at 129.249 kb on + strand at 129.418 kb on - strand at 129.999 kb on + strand, within AZOBR_RS30235 at 129.999 kb on + strand, within AZOBR_RS30235 at 129.999 kb on + strand, within AZOBR_RS30235 at 129.999 kb on + strand, within AZOBR_RS30235 at 129.999 kb on + strand, within AZOBR_RS30235 at 129.999 kb on + strand at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235 at 130.000 kb on - strand, within AZOBR_RS30235
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 127,521 + -2.2 127,522 - +0.2 127,563 + -0.3 127,625 + -0.2 127,625 + -0.1 127,626 - -1.7 127,626 - +0.5 127,763 + AZOBR_RS30225 0.26 +2.0 127,769 + AZOBR_RS30225 0.27 -0.1 127,769 + AZOBR_RS30225 0.27 -3.5 127,769 + AZOBR_RS30225 0.27 -0.2 127,769 + AZOBR_RS30225 0.27 -0.8 127,770 - AZOBR_RS30225 0.27 -0.1 127,770 - AZOBR_RS30225 0.27 -2.0 127,770 - AZOBR_RS30225 0.27 -0.6 127,770 - AZOBR_RS30225 0.27 +0.3 127,892 + AZOBR_RS30225 0.41 -0.0 127,892 + AZOBR_RS30225 0.41 +2.8 127,892 + AZOBR_RS30225 0.41 -0.7 127,893 - AZOBR_RS30225 0.41 +0.3 127,893 - AZOBR_RS30225 0.41 -0.1 127,893 - AZOBR_RS30225 0.41 -0.7 128,003 + AZOBR_RS30225 0.55 +0.2 128,003 + AZOBR_RS30225 0.55 -3.4 128,004 - AZOBR_RS30225 0.55 -1.2 128,069 + AZOBR_RS30225 0.62 -0.6 128,070 - AZOBR_RS30225 0.63 +1.7 128,070 - AZOBR_RS30225 0.63 +0.6 128,070 - AZOBR_RS30225 0.63 +0.6 128,070 - AZOBR_RS30225 0.63 -0.0 128,261 + AZOBR_RS30225 0.85 -3.9 128,261 + AZOBR_RS30225 0.85 -1.2 128,262 - AZOBR_RS30225 0.86 -2.3 128,262 - AZOBR_RS30225 0.86 +0.6 128,333 + -4.7 128,334 - -3.5 128,693 - AZOBR_RS30230 0.34 +1.9 128,840 - AZOBR_RS30230 0.55 -0.2 129,135 + +0.9 129,135 + -0.6 129,135 + +0.1 129,249 + +1.2 129,418 - +1.1 129,999 + AZOBR_RS30235 0.60 +0.7 129,999 + AZOBR_RS30235 0.60 +0.7 129,999 + AZOBR_RS30235 0.60 +0.6 129,999 + AZOBR_RS30235 0.60 +1.5 129,999 + AZOBR_RS30235 0.60 +0.4 129,999 + +1.1 130,000 - AZOBR_RS30235 0.61 +0.5 130,000 - AZOBR_RS30235 0.61 -3.5 130,000 - AZOBR_RS30235 0.61 -3.1 130,000 - AZOBR_RS30235 0.61 +1.0 130,000 - AZOBR_RS30235 0.61 +0.1 130,000 - AZOBR_RS30235 0.61 -0.9 130,000 - AZOBR_RS30235 0.61 +0.3 130,000 - AZOBR_RS30235 0.61 +0.3
Or see this region's nucleotide sequence