Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS24540 and AZOBR_RS24545 overlap by 4 nucleotides AZOBR_RS24545 and AZOBR_RS24550 are separated by 13 nucleotides AZOBR_RS24550 and AZOBR_RS24555 are separated by 39 nucleotides
AZOBR_RS24540: AZOBR_RS24540 - formylmethanofuran--tetrahydromethanopterin formyltransferase, at 564,176 to 565,075
_RS24540
AZOBR_RS24545: AZOBR_RS24545 - putative formylmethanofuran dehydrogenase subunit C, at 565,072 to 565,896
_RS24545
AZOBR_RS24550: AZOBR_RS24550 - branched-chain amino acid ABC transporter substrate-binding protein, at 565,910 to 567,100
_RS24550
AZOBR_RS24555: AZOBR_RS24555 - membrane protein, at 567,140 to 568,120
_RS24555
Position (kb)
565
566
567
568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 565.348 kb on + strand, within AZOBR_RS24545 at 565.839 kb on + strand at 566.004 kb on - strand at 566.591 kb on - strand, within AZOBR_RS24550 at 566.591 kb on - strand, within AZOBR_RS24550 at 566.657 kb on + strand, within AZOBR_RS24550 at 566.657 kb on + strand, within AZOBR_RS24550 at 566.658 kb on - strand, within AZOBR_RS24550 at 566.658 kb on - strand, within AZOBR_RS24550 at 566.811 kb on - strand, within AZOBR_RS24550 at 566.811 kb on - strand, within AZOBR_RS24550 at 567.029 kb on + strand at 567.177 kb on + strand at 567.479 kb on + strand, within AZOBR_RS24555 at 567.479 kb on + strand, within AZOBR_RS24555 at 567.480 kb on - strand, within AZOBR_RS24555 at 567.555 kb on + strand, within AZOBR_RS24555 at 567.555 kb on + strand, within AZOBR_RS24555 at 567.628 kb on + strand, within AZOBR_RS24555 at 567.628 kb on + strand, within AZOBR_RS24555 at 567.628 kb on + strand, within AZOBR_RS24555 at 567.629 kb on - strand, within AZOBR_RS24555 at 567.629 kb on - strand, within AZOBR_RS24555 at 567.932 kb on + strand, within AZOBR_RS24555 at 567.933 kb on - strand, within AZOBR_RS24555 at 567.933 kb on - strand, within AZOBR_RS24555 at 567.933 kb on - strand, within AZOBR_RS24555 at 567.948 kb on - strand, within AZOBR_RS24555 at 568.007 kb on + strand, within AZOBR_RS24555 at 568.007 kb on + strand, within AZOBR_RS24555 at 568.008 kb on - strand, within AZOBR_RS24555 at 568.008 kb on - strand, within AZOBR_RS24555
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 565,348 + AZOBR_RS24545 0.33 -3.1 565,839 + -0.6 566,004 - +1.8 566,591 - AZOBR_RS24550 0.57 +1.7 566,591 - AZOBR_RS24550 0.57 -0.3 566,657 + AZOBR_RS24550 0.63 -0.3 566,657 + AZOBR_RS24550 0.63 +0.1 566,658 - AZOBR_RS24550 0.63 +2.3 566,658 - AZOBR_RS24550 0.63 +0.1 566,811 - AZOBR_RS24550 0.76 +3.6 566,811 - AZOBR_RS24550 0.76 +0.2 567,029 + +0.3 567,177 + +2.3 567,479 + AZOBR_RS24555 0.35 -1.0 567,479 + AZOBR_RS24555 0.35 +0.2 567,480 - AZOBR_RS24555 0.35 -1.0 567,555 + AZOBR_RS24555 0.42 +1.6 567,555 + AZOBR_RS24555 0.42 +0.9 567,628 + AZOBR_RS24555 0.50 -2.0 567,628 + AZOBR_RS24555 0.50 -3.0 567,628 + AZOBR_RS24555 0.50 -0.4 567,629 - AZOBR_RS24555 0.50 +2.4 567,629 - AZOBR_RS24555 0.50 +0.7 567,932 + AZOBR_RS24555 0.81 +0.3 567,933 - AZOBR_RS24555 0.81 -0.5 567,933 - AZOBR_RS24555 0.81 -2.2 567,933 - AZOBR_RS24555 0.81 -0.1 567,948 - AZOBR_RS24555 0.82 +0.3 568,007 + AZOBR_RS24555 0.88 +0.9 568,007 + AZOBR_RS24555 0.88 +0.9 568,008 - AZOBR_RS24555 0.88 +0.1 568,008 - AZOBR_RS24555 0.88 -0.4
Or see this region's nucleotide sequence