Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS21710 and AZOBR_RS21715 are separated by 8 nucleotides AZOBR_RS21715 and AZOBR_RS21720 are separated by 233 nucleotides AZOBR_RS21720 and AZOBR_RS21725 are separated by 48 nucleotides
AZOBR_RS21710: AZOBR_RS21710 - conserved membrane protein of unknown function, at 1,648,023 to 1,648,436
_RS21710
AZOBR_RS21715: AZOBR_RS21715 - hypothetical protein, at 1,648,445 to 1,649,116
_RS21715
AZOBR_RS21720: AZOBR_RS21720 - hypothetical protein, at 1,649,350 to 1,650,225
_RS21720
AZOBR_RS21725: AZOBR_RS21725 - imidazolonepropionase, at 1,650,274 to 1,651,488
_RS21725
Position (kb)
1649
1650
1651 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1648.438 kb on + strand at 1648.551 kb on + strand, within AZOBR_RS21715 at 1648.551 kb on + strand, within AZOBR_RS21715 at 1648.552 kb on - strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.929 kb on + strand, within AZOBR_RS21715 at 1649.265 kb on - strand at 1649.273 kb on + strand at 1649.273 kb on + strand at 1649.315 kb on + strand at 1649.315 kb on + strand at 1649.315 kb on + strand at 1649.315 kb on + strand at 1649.316 kb on - strand at 1649.348 kb on + strand at 1649.348 kb on + strand at 1649.348 kb on + strand at 1649.348 kb on + strand at 1649.349 kb on - strand at 1649.349 kb on - strand at 1649.349 kb on - strand at 1649.432 kb on + strand at 1649.432 kb on + strand at 1649.432 kb on + strand at 1649.433 kb on - strand at 1649.433 kb on - strand at 1649.433 kb on - strand at 1649.588 kb on + strand, within AZOBR_RS21720 at 1649.589 kb on - strand, within AZOBR_RS21720 at 1649.738 kb on + strand, within AZOBR_RS21720 at 1649.738 kb on + strand, within AZOBR_RS21720 at 1649.738 kb on + strand, within AZOBR_RS21720 at 1649.739 kb on - strand, within AZOBR_RS21720 at 1649.822 kb on + strand, within AZOBR_RS21720 at 1649.822 kb on + strand, within AZOBR_RS21720 at 1649.822 kb on + strand, within AZOBR_RS21720 at 1649.823 kb on - strand, within AZOBR_RS21720 at 1649.823 kb on - strand, within AZOBR_RS21720 at 1650.068 kb on + strand, within AZOBR_RS21720 at 1650.069 kb on - strand, within AZOBR_RS21720 at 1650.158 kb on + strand at 1650.233 kb on + strand at 1650.233 kb on + strand at 1650.233 kb on + strand at 1650.233 kb on + strand at 1650.233 kb on + strand at 1650.233 kb on + strand at 1650.234 kb on - strand at 1650.234 kb on - strand at 1650.234 kb on - strand at 1650.234 kb on - strand at 1650.234 kb on - strand at 1650.536 kb on - strand, within AZOBR_RS21725 at 1650.823 kb on + strand, within AZOBR_RS21725 at 1650.823 kb on + strand, within AZOBR_RS21725 at 1650.824 kb on - strand, within AZOBR_RS21725 at 1650.824 kb on - strand, within AZOBR_RS21725 at 1651.027 kb on + strand, within AZOBR_RS21725 at 1651.028 kb on - strand, within AZOBR_RS21725 at 1651.028 kb on - strand, within AZOBR_RS21725 at 1651.130 kb on - strand, within AZOBR_RS21725
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 1,648,438 + -2.2 1,648,551 + AZOBR_RS21715 0.16 +0.8 1,648,551 + AZOBR_RS21715 0.16 -1.9 1,648,552 - AZOBR_RS21715 0.16 -0.6 1,648,788 + AZOBR_RS21715 0.51 -0.5 1,648,788 + AZOBR_RS21715 0.51 +1.0 1,648,788 + AZOBR_RS21715 0.51 -0.2 1,648,789 - AZOBR_RS21715 0.51 +0.8 1,648,789 - AZOBR_RS21715 0.51 -0.3 1,648,789 - AZOBR_RS21715 0.51 +0.3 1,648,929 + AZOBR_RS21715 0.72 +0.0 1,649,265 - -0.1 1,649,273 + +0.1 1,649,273 + -1.1 1,649,315 + +1.1 1,649,315 + +1.1 1,649,315 + +0.3 1,649,315 + +1.6 1,649,316 - -0.5 1,649,348 + -0.2 1,649,348 + +0.9 1,649,348 + +0.4 1,649,348 + +1.2 1,649,349 - -0.2 1,649,349 - +1.2 1,649,349 - -0.9 1,649,432 + -0.3 1,649,432 + +1.7 1,649,432 + +1.3 1,649,433 - -1.3 1,649,433 - +0.2 1,649,433 - -0.3 1,649,588 + AZOBR_RS21720 0.27 -4.2 1,649,589 - AZOBR_RS21720 0.27 +0.5 1,649,738 + AZOBR_RS21720 0.44 +3.5 1,649,738 + AZOBR_RS21720 0.44 +1.4 1,649,738 + AZOBR_RS21720 0.44 -0.6 1,649,739 - AZOBR_RS21720 0.44 -3.2 1,649,822 + AZOBR_RS21720 0.54 +1.3 1,649,822 + AZOBR_RS21720 0.54 +1.3 1,649,822 + AZOBR_RS21720 0.54 -0.3 1,649,823 - AZOBR_RS21720 0.54 +0.4 1,649,823 - AZOBR_RS21720 0.54 -3.6 1,650,068 + AZOBR_RS21720 0.82 -0.8 1,650,069 - AZOBR_RS21720 0.82 -3.2 1,650,158 + -0.5 1,650,233 + -2.8 1,650,233 + -0.3 1,650,233 + +1.6 1,650,233 + +0.3 1,650,233 + -0.1 1,650,233 + +1.5 1,650,234 - +1.5 1,650,234 - +1.3 1,650,234 - +0.8 1,650,234 - +1.9 1,650,234 - -0.0 1,650,536 - AZOBR_RS21725 0.22 -0.4 1,650,823 + AZOBR_RS21725 0.45 +1.9 1,650,823 + AZOBR_RS21725 0.45 -0.2 1,650,824 - AZOBR_RS21725 0.45 -1.5 1,650,824 - AZOBR_RS21725 0.45 +0.0 1,651,027 + AZOBR_RS21725 0.62 -2.0 1,651,028 - AZOBR_RS21725 0.62 -0.3 1,651,028 - AZOBR_RS21725 0.62 +1.4 1,651,130 - AZOBR_RS21725 0.70 -0.2
Or see this region's nucleotide sequence