Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS21695 and AZOBR_RS21700 are separated by 190 nucleotides AZOBR_RS21700 and AZOBR_RS21705 overlap by 4 nucleotides AZOBR_RS21705 and AZOBR_RS21710 overlap by 4 nucleotides AZOBR_RS21710 and AZOBR_RS21715 are separated by 8 nucleotides
AZOBR_RS21695: AZOBR_RS21695 - hypothetical protein, at 1,645,204 to 1,645,683
_RS21695
AZOBR_RS21700: AZOBR_RS21700 - hypothetical protein, at 1,645,874 to 1,646,392
_RS21700
AZOBR_RS21705: AZOBR_RS21705 - 2-aminobenzoate-CoA ligase, at 1,646,389 to 1,648,026
_RS21705
AZOBR_RS21710: AZOBR_RS21710 - conserved membrane protein of unknown function, at 1,648,023 to 1,648,436
_RS21710
AZOBR_RS21715: AZOBR_RS21715 - hypothetical protein, at 1,648,445 to 1,649,116
_RS21715
Position (kb)
1646
1647
1648
1649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1645.666 kb on + strand at 1645.788 kb on + strand at 1645.789 kb on - strand at 1645.789 kb on - strand at 1645.794 kb on - strand at 1645.794 kb on - strand at 1645.794 kb on - strand at 1645.794 kb on - strand at 1646.157 kb on + strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.158 kb on - strand, within AZOBR_RS21700 at 1646.587 kb on + strand, within AZOBR_RS21705 at 1647.616 kb on + strand, within AZOBR_RS21705 at 1647.616 kb on + strand, within AZOBR_RS21705 at 1647.617 kb on - strand, within AZOBR_RS21705 at 1647.892 kb on + strand at 1648.197 kb on + strand, within AZOBR_RS21710 at 1648.198 kb on - strand, within AZOBR_RS21710 at 1648.198 kb on - strand, within AZOBR_RS21710 at 1648.198 kb on - strand, within AZOBR_RS21710 at 1648.338 kb on + strand, within AZOBR_RS21710 at 1648.438 kb on + strand at 1648.551 kb on + strand, within AZOBR_RS21715 at 1648.551 kb on + strand, within AZOBR_RS21715 at 1648.552 kb on - strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.788 kb on + strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.789 kb on - strand, within AZOBR_RS21715 at 1648.929 kb on + strand, within AZOBR_RS21715
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 1,645,666 + +0.8 1,645,788 + +0.7 1,645,789 - +0.9 1,645,789 - +0.4 1,645,794 - +0.8 1,645,794 - +0.5 1,645,794 - +1.1 1,645,794 - +1.5 1,646,157 + AZOBR_RS21700 0.55 +0.2 1,646,158 - AZOBR_RS21700 0.55 +1.3 1,646,158 - AZOBR_RS21700 0.55 +0.9 1,646,158 - AZOBR_RS21700 0.55 -0.3 1,646,158 - AZOBR_RS21700 0.55 +1.1 1,646,158 - AZOBR_RS21700 0.55 +1.0 1,646,158 - AZOBR_RS21700 0.55 +1.1 1,646,587 + AZOBR_RS21705 0.12 +0.9 1,647,616 + AZOBR_RS21705 0.75 +1.9 1,647,616 + AZOBR_RS21705 0.75 -2.4 1,647,617 - AZOBR_RS21705 0.75 +2.3 1,647,892 + +1.5 1,648,197 + AZOBR_RS21710 0.42 +0.6 1,648,198 - AZOBR_RS21710 0.42 -3.2 1,648,198 - AZOBR_RS21710 0.42 +0.4 1,648,198 - AZOBR_RS21710 0.42 +0.7 1,648,338 + AZOBR_RS21710 0.76 -2.6 1,648,438 + -2.2 1,648,551 + AZOBR_RS21715 0.16 +0.8 1,648,551 + AZOBR_RS21715 0.16 -1.9 1,648,552 - AZOBR_RS21715 0.16 -0.6 1,648,788 + AZOBR_RS21715 0.51 -0.5 1,648,788 + AZOBR_RS21715 0.51 +1.0 1,648,788 + AZOBR_RS21715 0.51 -0.2 1,648,789 - AZOBR_RS21715 0.51 +0.8 1,648,789 - AZOBR_RS21715 0.51 -0.3 1,648,789 - AZOBR_RS21715 0.51 +0.3 1,648,929 + AZOBR_RS21715 0.72 +0.0
Or see this region's nucleotide sequence