Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16700 and AZOBR_RS16705 are separated by 107 nucleotides AZOBR_RS16705 and AZOBR_RS16710 are separated by 2 nucleotides AZOBR_RS16710 and AZOBR_RS16715 are separated by 2 nucleotides
AZOBR_RS16700: AZOBR_RS16700 - precorrin-3B C17-methyltransferase, at 505,354 to 506,133
_RS16700
AZOBR_RS16705: AZOBR_RS16705 - cobalt-precorrin-6x reductase, at 506,241 to 506,975
_RS16705
AZOBR_RS16710: AZOBR_RS16710 - precorrin-6Y C5 15-methyltransferase, at 506,978 to 508,225
_RS16710
AZOBR_RS16715: AZOBR_RS16715 - cobalamin biosynthesis protein, at 508,228 to 508,632
_RS16715
Position (kb) 506
507
508
509 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 506.101 kb on + strand at 506.101 kb on + strand at 506.102 kb on - strand at 506.117 kb on - strand at 506.243 kb on - strand at 506.477 kb on - strand, within AZOBR_RS16705 at 506.477 kb on - strand, within AZOBR_RS16705 at 506.528 kb on - strand, within AZOBR_RS16705 at 507.005 kb on - strand at 507.005 kb on - strand at 507.260 kb on + strand, within AZOBR_RS16710 at 507.261 kb on - strand, within AZOBR_RS16710 at 508.888 kb on + strand at 509.170 kb on + strand at 509.170 kb on + strand at 509.170 kb on + strand at 509.171 kb on - strand at 509.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 506,101 + -2.7 506,101 + -2.9 506,102 - +1.2 506,117 - +2.1 506,243 - -0.3 506,477 - AZOBR_RS16705 0.32 -2.8 506,477 - AZOBR_RS16705 0.32 +0.9 506,528 - AZOBR_RS16705 0.39 +1.2 507,005 - -2.4 507,005 - +1.1 507,260 + AZOBR_RS16710 0.23 +0.5 507,261 - AZOBR_RS16710 0.23 +1.1 508,888 + +0.8 509,170 + +0.7 509,170 + -2.6 509,170 + +0.9 509,171 - -0.7 509,171 - +2.6
Or see this region's nucleotide sequence