Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16255 and AZOBR_RS16260 are separated by 40 nucleotides AZOBR_RS16260 and AZOBR_RS16265 are separated by 77 nucleotides AZOBR_RS16265 and AZOBR_RS16270 are separated by 22 nucleotides AZOBR_RS16270 and AZOBR_RS16275 are separated by 27 nucleotides
AZOBR_RS16255: AZOBR_RS16255 - membrane protein, at 398,482 to 398,964
_RS16255
AZOBR_RS16260: AZOBR_RS16260 - azoreductase, at 399,005 to 399,604
_RS16260
AZOBR_RS16265: AZOBR_RS16265 - 3-oxoacyl-ACP reductase, at 399,682 to 400,461
_RS16265
AZOBR_RS16270: AZOBR_RS16270 - DNA replication protein, at 400,484 to 401,164
_RS16270
AZOBR_RS16275: AZOBR_RS16275 - membrane protein, at 401,192 to 402,253
_RS16275
Position (kb)
399
400
401 Strain fitness (log2 ratio)
-1
0
1 at 398.734 kb on + strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 399.266 kb on + strand, within AZOBR_RS16260 at 399.267 kb on - strand, within AZOBR_RS16260 at 399.408 kb on + strand, within AZOBR_RS16260 at 399.409 kb on - strand, within AZOBR_RS16260 at 401.212 kb on - strand at 401.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 398,734 + AZOBR_RS16255 0.52 +1.0 398,735 - AZOBR_RS16255 0.52 +0.5 398,735 - AZOBR_RS16255 0.52 +1.3 398,735 - AZOBR_RS16255 0.52 +0.5 399,266 + AZOBR_RS16260 0.43 +0.1 399,267 - AZOBR_RS16260 0.44 +0.3 399,408 + AZOBR_RS16260 0.67 -0.6 399,409 - AZOBR_RS16260 0.67 +0.6 401,212 - +0.7 401,215 - +0.7
Or see this region's nucleotide sequence