Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS14865 and AZOBR_RS14870 are separated by 85 nucleotides AZOBR_RS14870 and AZOBR_RS14875 are separated by 129 nucleotides
AZOBR_RS14865: AZOBR_RS14865 - aminopeptidase N, at 107,728 to 110,385
_RS14865
AZOBR_RS14870: AZOBR_RS14870 - cytochrome C transmembrane protein, at 110,471 to 110,851
_RS14870
AZOBR_RS14875: AZOBR_RS14875 - carbon monoxide dehydrogenase subunit G, at 110,981 to 111,643
_RS14875
Position (kb)
107
108
109
110
111 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 106.830 kb on - strand at 106.972 kb on + strand at 106.973 kb on - strand at 106.976 kb on + strand at 106.976 kb on + strand at 107.114 kb on - strand at 107.116 kb on - strand at 107.173 kb on - strand at 107.183 kb on + strand at 107.275 kb on + strand at 107.307 kb on + strand at 107.421 kb on - strand at 107.523 kb on + strand at 107.674 kb on - strand at 107.674 kb on - strand at 107.968 kb on + strand at 107.969 kb on - strand at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.076 kb on + strand, within AZOBR_RS14865 at 108.077 kb on - strand, within AZOBR_RS14865 at 108.077 kb on - strand, within AZOBR_RS14865 at 108.137 kb on - strand, within AZOBR_RS14865 at 108.137 kb on - strand, within AZOBR_RS14865 at 108.137 kb on - strand, within AZOBR_RS14865 at 108.137 kb on - strand, within AZOBR_RS14865 at 108.301 kb on + strand, within AZOBR_RS14865 at 108.301 kb on + strand, within AZOBR_RS14865 at 108.301 kb on + strand, within AZOBR_RS14865 at 108.302 kb on - strand, within AZOBR_RS14865 at 108.302 kb on - strand, within AZOBR_RS14865 at 108.302 kb on - strand, within AZOBR_RS14865 at 108.302 kb on - strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.388 kb on + strand, within AZOBR_RS14865 at 108.389 kb on - strand, within AZOBR_RS14865 at 108.389 kb on - strand, within AZOBR_RS14865 at 108.572 kb on - strand, within AZOBR_RS14865 at 108.572 kb on - strand, within AZOBR_RS14865 at 108.572 kb on - strand, within AZOBR_RS14865 at 108.572 kb on - strand, within AZOBR_RS14865 at 108.643 kb on + strand, within AZOBR_RS14865 at 108.643 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.853 kb on + strand, within AZOBR_RS14865 at 108.854 kb on - strand, within AZOBR_RS14865 at 108.854 kb on - strand, within AZOBR_RS14865 at 108.854 kb on - strand, within AZOBR_RS14865 at 108.854 kb on - strand, within AZOBR_RS14865 at 108.854 kb on - strand, within AZOBR_RS14865 at 108.874 kb on + strand, within AZOBR_RS14865 at 108.875 kb on - strand, within AZOBR_RS14865 at 108.889 kb on + strand, within AZOBR_RS14865 at 108.889 kb on + strand, within AZOBR_RS14865 at 108.889 kb on + strand, within AZOBR_RS14865 at 108.920 kb on - strand, within AZOBR_RS14865 at 108.920 kb on - strand, within AZOBR_RS14865 at 108.920 kb on - strand, within AZOBR_RS14865 at 108.967 kb on + strand, within AZOBR_RS14865 at 108.968 kb on - strand, within AZOBR_RS14865 at 109.631 kb on - strand, within AZOBR_RS14865 at 109.874 kb on - strand, within AZOBR_RS14865 at 109.874 kb on - strand, within AZOBR_RS14865 at 109.969 kb on + strand, within AZOBR_RS14865 at 109.969 kb on + strand, within AZOBR_RS14865 at 110.111 kb on - strand, within AZOBR_RS14865 at 110.111 kb on - strand, within AZOBR_RS14865 at 110.580 kb on + strand, within AZOBR_RS14870 at 110.580 kb on + strand, within AZOBR_RS14870 at 110.581 kb on - strand, within AZOBR_RS14870 at 110.840 kb on - strand at 110.840 kb on - strand at 110.840 kb on - strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.915 kb on - strand at 110.915 kb on - strand at 110.915 kb on - strand at 110.929 kb on + strand at 110.930 kb on - strand at 111.045 kb on + strand at 111.045 kb on + strand at 111.046 kb on - strand at 111.046 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 106,830 - -0.6 106,972 + +1.6 106,973 - -4.3 106,976 + +0.6 106,976 + +0.4 107,114 - +1.3 107,116 - -2.7 107,173 - -0.7 107,183 + +0.2 107,275 + -0.2 107,307 + +0.6 107,421 - +0.4 107,523 + +1.5 107,674 - -0.4 107,674 - -0.0 107,968 + -0.1 107,969 - +0.4 108,076 + AZOBR_RS14865 0.13 +1.1 108,076 + AZOBR_RS14865 0.13 -0.1 108,076 + AZOBR_RS14865 0.13 -0.2 108,076 + AZOBR_RS14865 0.13 -0.5 108,076 + AZOBR_RS14865 0.13 +0.4 108,076 + AZOBR_RS14865 0.13 +0.9 108,076 + AZOBR_RS14865 0.13 -0.3 108,077 - AZOBR_RS14865 0.13 -2.9 108,077 - AZOBR_RS14865 0.13 +0.6 108,137 - AZOBR_RS14865 0.15 +0.2 108,137 - AZOBR_RS14865 0.15 -1.3 108,137 - AZOBR_RS14865 0.15 -0.8 108,137 - AZOBR_RS14865 0.15 -2.5 108,301 + AZOBR_RS14865 0.22 -1.7 108,301 + AZOBR_RS14865 0.22 +0.1 108,301 + AZOBR_RS14865 0.22 +2.1 108,302 - AZOBR_RS14865 0.22 -0.8 108,302 - AZOBR_RS14865 0.22 +1.9 108,302 - AZOBR_RS14865 0.22 -0.8 108,302 - AZOBR_RS14865 0.22 -0.4 108,388 + AZOBR_RS14865 0.25 +1.1 108,388 + AZOBR_RS14865 0.25 +0.0 108,388 + AZOBR_RS14865 0.25 -1.3 108,388 + AZOBR_RS14865 0.25 -0.2 108,388 + AZOBR_RS14865 0.25 +1.3 108,388 + AZOBR_RS14865 0.25 -1.7 108,389 - AZOBR_RS14865 0.25 -1.6 108,389 - AZOBR_RS14865 0.25 +0.6 108,572 - AZOBR_RS14865 0.32 +0.3 108,572 - AZOBR_RS14865 0.32 -0.9 108,572 - AZOBR_RS14865 0.32 +1.2 108,572 - AZOBR_RS14865 0.32 -0.6 108,643 + AZOBR_RS14865 0.34 -0.3 108,643 + AZOBR_RS14865 0.34 -2.6 108,853 + AZOBR_RS14865 0.42 -0.8 108,853 + AZOBR_RS14865 0.42 +0.8 108,853 + AZOBR_RS14865 0.42 -1.5 108,853 + AZOBR_RS14865 0.42 +0.4 108,853 + AZOBR_RS14865 0.42 -1.9 108,853 + AZOBR_RS14865 0.42 +0.9 108,854 - AZOBR_RS14865 0.42 -3.8 108,854 - AZOBR_RS14865 0.42 -1.9 108,854 - AZOBR_RS14865 0.42 -1.1 108,854 - AZOBR_RS14865 0.42 +0.7 108,854 - AZOBR_RS14865 0.42 +0.7 108,874 + AZOBR_RS14865 0.43 -0.3 108,875 - AZOBR_RS14865 0.43 -3.5 108,889 + AZOBR_RS14865 0.44 -0.8 108,889 + AZOBR_RS14865 0.44 +1.2 108,889 + AZOBR_RS14865 0.44 +0.7 108,920 - AZOBR_RS14865 0.45 +0.1 108,920 - AZOBR_RS14865 0.45 +0.3 108,920 - AZOBR_RS14865 0.45 +0.1 108,967 + AZOBR_RS14865 0.47 -2.3 108,968 - AZOBR_RS14865 0.47 -1.4 109,631 - AZOBR_RS14865 0.72 -1.1 109,874 - AZOBR_RS14865 0.81 -1.1 109,874 - AZOBR_RS14865 0.81 -2.9 109,969 + AZOBR_RS14865 0.84 +0.5 109,969 + AZOBR_RS14865 0.84 -0.4 110,111 - AZOBR_RS14865 0.90 +0.5 110,111 - AZOBR_RS14865 0.90 -0.5 110,580 + AZOBR_RS14870 0.29 +0.3 110,580 + AZOBR_RS14870 0.29 +0.3 110,581 - AZOBR_RS14870 0.29 +2.1 110,840 - +2.0 110,840 - -3.1 110,840 - -1.5 110,914 + -2.9 110,914 + -1.0 110,914 + -0.4 110,914 + +0.9 110,915 - +0.4 110,915 - -0.5 110,915 - +0.6 110,929 + -0.1 110,930 - -0.1 111,045 + -0.9 111,045 + +0.8 111,046 - +1.6 111,046 - +0.1
Or see this region's nucleotide sequence