Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS14440 and AZOBR_RS14445 are separated by 301 nucleotides AZOBR_RS14445 and AZOBR_RS14450 are separated by 305 nucleotides AZOBR_RS14450 and AZOBR_RS14455 are separated by 42 nucleotides AZOBR_RS14455 and AZOBR_RS14460 overlap by 4 nucleotides
AZOBR_RS14440: AZOBR_RS14440 - ATPase AAA, at 12,985 to 14,385
_RS14440
AZOBR_RS14445: AZOBR_RS14445 - hypothetical protein, at 14,687 to 14,881
_RS14445
AZOBR_RS14450: AZOBR_RS14450 - potassium transporter TrkA, at 15,187 to 16,563
_RS14450
AZOBR_RS14455: AZOBR_RS14455 - D-amino acid aminotransferase, at 16,606 to 17,469
_RS14455
AZOBR_RS14460: AZOBR_RS14460 - HAD family hydrolase, at 17,466 to 18,164
_RS14460
Position (kb) 15
16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 14.700 kb on - strand at 14.957 kb on + strand at 15.105 kb on + strand at 15.148 kb on + strand at 15.496 kb on + strand, within AZOBR_RS14450 at 15.817 kb on + strand, within AZOBR_RS14450 at 15.952 kb on + strand, within AZOBR_RS14450 at 15.953 kb on - strand, within AZOBR_RS14450 at 16.201 kb on + strand, within AZOBR_RS14450 at 16.201 kb on + strand, within AZOBR_RS14450 at 16.201 kb on + strand at 16.201 kb on + strand, within AZOBR_RS14450 at 16.202 kb on - strand, within AZOBR_RS14450 at 16.621 kb on + strand at 16.621 kb on + strand at 16.621 kb on + strand at 16.621 kb on + strand at 16.621 kb on + strand at 16.622 kb on - strand at 16.637 kb on - strand at 16.873 kb on + strand, within AZOBR_RS14455 at 16.874 kb on - strand, within AZOBR_RS14455 at 16.874 kb on - strand, within AZOBR_RS14455 at 17.443 kb on + strand at 17.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 14,700 - -0.4 14,957 + -1.7 15,105 + -0.3 15,148 + +0.3 15,496 + AZOBR_RS14450 0.22 -0.8 15,817 + AZOBR_RS14450 0.46 -1.5 15,952 + AZOBR_RS14450 0.56 -2.1 15,953 - AZOBR_RS14450 0.56 +1.7 16,201 + AZOBR_RS14450 0.74 -2.8 16,201 + AZOBR_RS14450 0.74 -1.5 16,201 + +0.9 16,201 + AZOBR_RS14450 0.74 -2.3 16,202 - AZOBR_RS14450 0.74 +0.0 16,621 + -0.1 16,621 + +0.4 16,621 + -2.2 16,621 + +1.1 16,621 + +1.9 16,622 - +0.5 16,637 - -1.4 16,873 + AZOBR_RS14455 0.31 +0.6 16,874 - AZOBR_RS14455 0.31 +0.0 16,874 - AZOBR_RS14455 0.31 +0.9 17,443 + +0.2 17,524 - -0.3
Or see this region's nucleotide sequence