Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS08070 and AZOBR_RS08075 are separated by 18 nucleotides AZOBR_RS08075 and AZOBR_RS08080 overlap by 4 nucleotides AZOBR_RS08080 and AZOBR_RS08085 are separated by 49 nucleotides AZOBR_RS08085 and AZOBR_RS08090 overlap by 4 nucleotides
AZOBR_RS08070: AZOBR_RS08070 - urease subunit alpha, at 1,701,946 to 1,703,655
_RS08070
AZOBR_RS08075: AZOBR_RS08075 - glutamate racemase, at 1,703,674 to 1,704,525
_RS08075
AZOBR_RS08080: AZOBR_RS08080 - urease accessory protein UreE, at 1,704,522 to 1,705,103
_RS08080
AZOBR_RS08085: AZOBR_RS08085 - urease accessory protein UreF, at 1,705,153 to 1,705,902
_RS08085
AZOBR_RS08090: AZOBR_RS08090 - urease accessory protein UreG, at 1,705,899 to 1,706,537
_RS08090
Position (kb) 1704
1705
1706 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1703.573 kb on - strand at 1703.641 kb on + strand at 1703.641 kb on + strand at 1703.642 kb on - strand at 1703.759 kb on - strand at 1703.785 kb on + strand, within AZOBR_RS08075 at 1703.786 kb on - strand, within AZOBR_RS08075 at 1703.786 kb on - strand, within AZOBR_RS08075 at 1703.812 kb on + strand, within AZOBR_RS08075 at 1703.812 kb on + strand, within AZOBR_RS08075 at 1703.812 kb on + strand, within AZOBR_RS08075 at 1703.812 kb on + strand, within AZOBR_RS08075 at 1703.812 kb on + strand, within AZOBR_RS08075 at 1703.813 kb on - strand, within AZOBR_RS08075 at 1704.214 kb on + strand, within AZOBR_RS08075 at 1704.215 kb on - strand, within AZOBR_RS08075 at 1704.376 kb on + strand, within AZOBR_RS08075 at 1704.377 kb on - strand, within AZOBR_RS08075 at 1704.732 kb on + strand, within AZOBR_RS08080 at 1704.961 kb on - strand, within AZOBR_RS08080 at 1705.282 kb on + strand, within AZOBR_RS08085 at 1705.879 kb on + strand at 1705.879 kb on + strand at 1705.880 kb on - strand at 1706.046 kb on + strand, within AZOBR_RS08090 at 1706.046 kb on + strand, within AZOBR_RS08090 at 1706.046 kb on + strand, within AZOBR_RS08090 at 1706.046 kb on + strand, within AZOBR_RS08090 at 1706.047 kb on - strand, within AZOBR_RS08090 at 1706.047 kb on - strand, within AZOBR_RS08090 at 1706.047 kb on - strand, within AZOBR_RS08090 at 1706.047 kb on - strand, within AZOBR_RS08090
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 1,703,573 - +1.6 1,703,641 + -3.1 1,703,641 + +0.4 1,703,642 - -0.1 1,703,759 - +0.7 1,703,785 + AZOBR_RS08075 0.13 +2.1 1,703,786 - AZOBR_RS08075 0.13 +2.3 1,703,786 - AZOBR_RS08075 0.13 +0.8 1,703,812 + AZOBR_RS08075 0.16 +1.4 1,703,812 + AZOBR_RS08075 0.16 -1.1 1,703,812 + AZOBR_RS08075 0.16 +0.1 1,703,812 + AZOBR_RS08075 0.16 +0.4 1,703,812 + AZOBR_RS08075 0.16 +1.5 1,703,813 - AZOBR_RS08075 0.16 +0.1 1,704,214 + AZOBR_RS08075 0.63 +1.6 1,704,215 - AZOBR_RS08075 0.63 +0.7 1,704,376 + AZOBR_RS08075 0.82 +1.8 1,704,377 - AZOBR_RS08075 0.83 -2.0 1,704,732 + AZOBR_RS08080 0.36 +0.4 1,704,961 - AZOBR_RS08080 0.75 -0.1 1,705,282 + AZOBR_RS08085 0.17 +0.2 1,705,879 + +0.1 1,705,879 + +1.4 1,705,880 - +0.3 1,706,046 + AZOBR_RS08090 0.23 +2.8 1,706,046 + AZOBR_RS08090 0.23 +0.8 1,706,046 + AZOBR_RS08090 0.23 +1.2 1,706,046 + AZOBR_RS08090 0.23 -1.1 1,706,047 - AZOBR_RS08090 0.23 -3.1 1,706,047 - AZOBR_RS08090 0.23 -1.8 1,706,047 - AZOBR_RS08090 0.23 -0.1 1,706,047 - AZOBR_RS08090 0.23 +0.5
Or see this region's nucleotide sequence