Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS06230 and AZOBR_RS06235 are separated by 100 nucleotides AZOBR_RS06235 and AZOBR_RS06240 are separated by 251 nucleotides AZOBR_RS06240 and AZOBR_RS06245 are separated by 5 nucleotides AZOBR_RS06245 and AZOBR_RS06250 are separated by 6 nucleotides
AZOBR_RS06230: AZOBR_RS06230 - hypothetical protein, at 1,317,923 to 1,319,881
_RS06230
AZOBR_RS06235: AZOBR_RS06235 - hypothetical protein, at 1,319,982 to 1,320,269
_RS06235
AZOBR_RS06240: AZOBR_RS06240 - hypothetical protein, at 1,320,521 to 1,321,024
_RS06240
AZOBR_RS06245: AZOBR_RS06245 - pyrroline-5-carboxylate reductase, at 1,321,030 to 1,321,857
_RS06245
AZOBR_RS06250: AZOBR_RS06250 - membrane protein, at 1,321,864 to 1,322,346
_RS06250
Position (kb) 1320
1321
1322 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1319.709 kb on + strand at 1319.710 kb on - strand at 1319.997 kb on - strand at 1320.412 kb on + strand at 1320.412 kb on + strand at 1320.413 kb on - strand at 1320.674 kb on + strand, within AZOBR_RS06240 at 1321.579 kb on + strand, within AZOBR_RS06245 at 1321.580 kb on - strand, within AZOBR_RS06245 at 1321.856 kb on - strand at 1321.856 kb on - strand at 1321.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 1,319,709 + +0.1 1,319,710 - +1.0 1,319,997 - -1.2 1,320,412 + +1.3 1,320,412 + +1.0 1,320,413 - +1.5 1,320,674 + AZOBR_RS06240 0.30 -1.1 1,321,579 + AZOBR_RS06245 0.66 -0.1 1,321,580 - AZOBR_RS06245 0.66 -2.9 1,321,856 - -0.4 1,321,856 - -1.0 1,321,856 - -3.0
Or see this region's nucleotide sequence