Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05505 and AZOBR_RS05510 are separated by 46 nucleotides AZOBR_RS05510 and AZOBR_RS05515 are separated by 305 nucleotides AZOBR_RS05515 and AZOBR_RS05520 are separated by 34 nucleotides AZOBR_RS05520 and AZOBR_RS05525 are separated by 98 nucleotides
AZOBR_RS05505: AZOBR_RS05505 - nitrogen fixation protein NifU, at 1,172,022 to 1,172,939
_RS05505
AZOBR_RS05510: AZOBR_RS05510 - hypothetical protein, at 1,172,986 to 1,173,366
_RS05510
AZOBR_RS05515: AZOBR_RS05515 - hypothetical protein, at 1,173,672 to 1,174,145
_RS05515
AZOBR_RS05520: AZOBR_RS05520 - hypothetical protein, at 1,174,180 to 1,174,740
_RS05520
AZOBR_RS05525: AZOBR_RS05525 - ferredoxin, at 1,174,839 to 1,175,132
_RS05525
Position (kb)
1173
1174
1175 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1172.929 kb on + strand at 1173.249 kb on - strand, within AZOBR_RS05510 at 1173.822 kb on + strand, within AZOBR_RS05515 at 1173.823 kb on - strand, within AZOBR_RS05515 at 1173.840 kb on + strand, within AZOBR_RS05515 at 1173.840 kb on + strand, within AZOBR_RS05515 at 1173.919 kb on - strand, within AZOBR_RS05515 at 1174.577 kb on + strand, within AZOBR_RS05520 at 1174.578 kb on - strand, within AZOBR_RS05520 at 1174.578 kb on - strand, within AZOBR_RS05520 at 1174.718 kb on + strand at 1174.718 kb on + strand at 1174.719 kb on - strand at 1174.824 kb on + strand at 1174.824 kb on + strand at 1174.825 kb on - strand at 1174.825 kb on - strand at 1174.825 kb on - strand at 1174.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 1,172,929 + -0.7 1,173,249 - AZOBR_RS05510 0.69 -1.4 1,173,822 + AZOBR_RS05515 0.32 +1.0 1,173,823 - AZOBR_RS05515 0.32 +1.0 1,173,840 + AZOBR_RS05515 0.35 -1.8 1,173,840 + AZOBR_RS05515 0.35 +1.4 1,173,919 - AZOBR_RS05515 0.52 +0.8 1,174,577 + AZOBR_RS05520 0.71 -0.6 1,174,578 - AZOBR_RS05520 0.71 -0.8 1,174,578 - AZOBR_RS05520 0.71 +2.2 1,174,718 + +1.0 1,174,718 + -1.1 1,174,719 - +1.5 1,174,824 + +0.5 1,174,824 + -1.4 1,174,825 - -0.2 1,174,825 - +0.2 1,174,825 - +0.3 1,174,825 - +0.8
Or see this region's nucleotide sequence