Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04255 and AZOBR_RS04260 are separated by 131 nucleotides AZOBR_RS04260 and AZOBR_RS04265 are separated by 346 nucleotides AZOBR_RS04265 and AZOBR_RS04270 are separated by 16 nucleotides AZOBR_RS04270 and AZOBR_RS04275 are separated by 53 nucleotides
AZOBR_RS04255: AZOBR_RS04255 - hypothetical protein, at 897,750 to 898,715
_RS04255
AZOBR_RS04260: AZOBR_RS04260 - hypothetical protein, at 898,847 to 899,227
_RS04260
AZOBR_RS04265: AZOBR_RS04265 - 2-isopropylmalate synthase, at 899,574 to 901,178
_RS04265
AZOBR_RS04270: AZOBR_RS04270 - RNA methyltransferase, at 901,195 to 901,965
_RS04270
AZOBR_RS04275: AZOBR_RS04275 - response regulator, at 902,019 to 902,732
_RS04275
Position (kb) 899
900
901
902 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 899.298 kb on + strand at 899.299 kb on - strand at 899.330 kb on + strand at 899.402 kb on - strand at 899.402 kb on - strand at 899.412 kb on + strand at 899.412 kb on + strand at 899.413 kb on - strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 900.069 kb on + strand, within AZOBR_RS04265 at 900.069 kb on + strand, within AZOBR_RS04265 at 900.522 kb on + strand, within AZOBR_RS04265 at 900.523 kb on - strand, within AZOBR_RS04265 at 900.523 kb on - strand, within AZOBR_RS04265 at 900.696 kb on + strand, within AZOBR_RS04265 at 900.696 kb on + strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.765 kb on + strand, within AZOBR_RS04265 at 900.766 kb on - strand, within AZOBR_RS04265 at 900.807 kb on + strand, within AZOBR_RS04265 at 900.877 kb on - strand, within AZOBR_RS04265 at 900.951 kb on + strand, within AZOBR_RS04265 at 901.042 kb on + strand at 901.043 kb on - strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.135 kb on - strand at 901.151 kb on + strand at 901.417 kb on + strand, within AZOBR_RS04270 at 901.418 kb on - strand, within AZOBR_RS04270 at 901.694 kb on - strand, within AZOBR_RS04270 at 901.963 kb on + strand at 902.008 kb on + strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.025 kb on + strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 898,576 + AZOBR_RS04255 0.86 +1.4 898,576 + AZOBR_RS04255 0.86 -1.3 898,576 + AZOBR_RS04255 0.86 -1.3 898,576 + AZOBR_RS04255 0.86 +0.6 898,576 + AZOBR_RS04255 0.86 +1.4 898,576 + AZOBR_RS04255 0.86 +1.0 898,576 + AZOBR_RS04255 0.86 -1.3 898,577 - AZOBR_RS04255 0.86 +0.4 898,577 - AZOBR_RS04255 0.86 +0.4 898,577 - AZOBR_RS04255 0.86 -0.6 898,577 - AZOBR_RS04255 0.86 -1.8 898,577 - AZOBR_RS04255 0.86 +1.9 898,577 - AZOBR_RS04255 0.86 +0.4 899,298 + +0.9 899,299 - +1.0 899,330 + -5.5 899,402 - +1.2 899,402 - -2.4 899,412 + -0.1 899,412 + -3.0 899,413 - -0.2 899,592 + -3.7 899,592 + -0.3 899,592 + +0.2 899,592 + +1.0 899,592 + -0.5 899,593 - +1.3 899,593 - +0.3 899,593 - +1.9 899,593 - -1.9 899,593 - +1.6 899,701 - -0.6 899,701 - -4.4 899,701 - +1.3 900,069 + AZOBR_RS04265 0.31 +0.6 900,069 + AZOBR_RS04265 0.31 -0.2 900,522 + AZOBR_RS04265 0.59 +0.1 900,523 - AZOBR_RS04265 0.59 -1.4 900,523 - AZOBR_RS04265 0.59 +1.6 900,696 + AZOBR_RS04265 0.70 -0.5 900,696 + AZOBR_RS04265 0.70 -0.6 900,697 - AZOBR_RS04265 0.70 -0.6 900,697 - AZOBR_RS04265 0.70 -0.1 900,697 - AZOBR_RS04265 0.70 -1.6 900,765 + AZOBR_RS04265 0.74 -2.3 900,766 - AZOBR_RS04265 0.74 +0.0 900,807 + AZOBR_RS04265 0.77 -0.6 900,877 - AZOBR_RS04265 0.81 -0.7 900,951 + AZOBR_RS04265 0.86 -0.4 901,042 + -0.2 901,043 - +0.6 901,134 + +0.9 901,134 + -2.3 901,134 + -0.3 901,135 - -0.2 901,151 + +0.9 901,417 + AZOBR_RS04270 0.29 -1.6 901,418 - AZOBR_RS04270 0.29 +0.5 901,694 - AZOBR_RS04270 0.65 -0.4 901,963 + +0.7 902,008 + +0.3 902,009 - -2.8 902,009 - -3.8 902,009 - +1.8 902,009 - -0.5 902,010 + -1.3 902,010 + -0.4 902,010 + +0.8 902,011 - -0.4 902,011 - +0.6 902,011 - +1.0 902,025 + -2.5 902,026 - -0.7 902,026 - +0.1 902,026 - +1.8 902,026 - -1.8 902,040 - -0.5
Or see this region's nucleotide sequence