Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03135 and AZOBR_RS03140 are separated by 12 nucleotides AZOBR_RS03140 and AZOBR_RS03145 are separated by 184 nucleotides AZOBR_RS03145 and AZOBR_RS03150 are separated by 147 nucleotides AZOBR_RS03150 and AZOBR_RS03155 are separated by 131 nucleotides
AZOBR_RS03135: AZOBR_RS03135 - orotidine 5'-phosphate decarboxylase, at 668,588 to 669,301
_RS03135
AZOBR_RS03140: AZOBR_RS03140 - N-(5'-phosphoribosyl)anthranilate isomerase, at 669,314 to 669,958
_RS03140
AZOBR_RS03145: AZOBR_RS03145 - diguanylate cyclase, at 670,143 to 671,018
_RS03145
AZOBR_RS03150: AZOBR_RS03150 - uracil phosphoribosyltransferase, at 671,166 to 671,816
_RS03150
AZOBR_RS03155: AZOBR_RS03155 - iron ABC transporter permease, at 671,948 to 673,672
_RS03155
Position (kb) 670
671
672 Strain fitness (log2 ratio) -2
-1
0
1
2 at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.300 kb on - strand at 669.313 kb on + strand at 669.390 kb on - strand at 670.036 kb on + strand at 670.037 kb on - strand at 670.437 kb on + strand, within AZOBR_RS03145 at 670.437 kb on + strand, within AZOBR_RS03145 at 670.662 kb on + strand, within AZOBR_RS03145 at 670.662 kb on + strand, within AZOBR_RS03145 at 670.959 kb on + strand at 671.308 kb on - strand, within AZOBR_RS03150 at 671.308 kb on - strand, within AZOBR_RS03150 at 671.887 kb on - strand at 671.940 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 669,299 + +1.7 669,299 + +0.5 669,299 + +0.3 669,299 + +0.7 669,300 - +1.5 669,313 + +1.1 669,390 - +1.6 670,036 + -0.5 670,037 - +0.2 670,437 + AZOBR_RS03145 0.34 -1.0 670,437 + AZOBR_RS03145 0.34 +2.3 670,662 + AZOBR_RS03145 0.59 -1.2 670,662 + AZOBR_RS03145 0.59 -0.8 670,959 + -2.0 671,308 - AZOBR_RS03150 0.22 -1.5 671,308 - AZOBR_RS03150 0.22 -2.3 671,887 - +0.7 671,940 - +0.8
Or see this region's nucleotide sequence