Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03125 and AZOBR_RS03130 are separated by 286 nucleotides AZOBR_RS03130 and AZOBR_RS03135 are separated by 260 nucleotides AZOBR_RS03135 and AZOBR_RS03140 are separated by 12 nucleotides
AZOBR_RS03125: AZOBR_RS03125 - FAD dependent oxidoreductase, at 666,305 to 667,417
_RS03125
AZOBR_RS03130: AZOBR_RS03130 - hypothetical protein, at 667,704 to 668,327
_RS03130
AZOBR_RS03135: AZOBR_RS03135 - orotidine 5'-phosphate decarboxylase, at 668,588 to 669,301
_RS03135
AZOBR_RS03140: AZOBR_RS03140 - N-(5'-phosphoribosyl)anthranilate isomerase, at 669,314 to 669,958
_RS03140
Position (kb) 667
668
669 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 666.974 kb on + strand, within AZOBR_RS03125 at 666.975 kb on - strand, within AZOBR_RS03125 at 667.055 kb on + strand, within AZOBR_RS03125 at 667.238 kb on + strand, within AZOBR_RS03125 at 667.238 kb on + strand, within AZOBR_RS03125 at 667.238 kb on + strand, within AZOBR_RS03125 at 667.239 kb on - strand, within AZOBR_RS03125 at 667.239 kb on - strand, within AZOBR_RS03125 at 667.548 kb on + strand at 667.548 kb on + strand at 667.548 kb on + strand at 667.548 kb on + strand at 667.548 kb on + strand at 667.548 kb on + strand at 667.731 kb on + strand at 667.731 kb on + strand at 667.732 kb on - strand at 668.128 kb on - strand, within AZOBR_RS03130 at 668.214 kb on - strand, within AZOBR_RS03130 at 668.539 kb on + strand at 668.868 kb on + strand, within AZOBR_RS03135 at 668.869 kb on - strand at 668.869 kb on - strand, within AZOBR_RS03135 at 668.869 kb on - strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.300 kb on - strand at 669.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 666,974 + AZOBR_RS03125 0.60 +0.8 666,975 - AZOBR_RS03125 0.60 -1.6 667,055 + AZOBR_RS03125 0.67 +0.3 667,238 + AZOBR_RS03125 0.84 -2.4 667,238 + AZOBR_RS03125 0.84 -0.2 667,238 + AZOBR_RS03125 0.84 +0.2 667,239 - AZOBR_RS03125 0.84 -0.8 667,239 - AZOBR_RS03125 0.84 -0.4 667,548 + +0.1 667,548 + +0.6 667,548 + +0.6 667,548 + -0.2 667,548 + +1.0 667,548 + -0.6 667,731 + -2.6 667,731 + -0.1 667,732 - +0.9 668,128 - AZOBR_RS03130 0.68 +0.3 668,214 - AZOBR_RS03130 0.82 +0.6 668,539 + -3.8 668,868 + AZOBR_RS03135 0.39 +1.1 668,869 - +1.2 668,869 - AZOBR_RS03135 0.39 +0.7 668,869 - -0.4 669,299 + +1.7 669,299 + +0.5 669,299 + +0.3 669,299 + +0.7 669,300 - +1.5 669,313 + +1.1
Or see this region's nucleotide sequence