Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01735 and AZOBR_RS01740 are separated by 6 nucleotides AZOBR_RS01740 and AZOBR_RS01745 are separated by 99 nucleotides AZOBR_RS01745 and AZOBR_RS01750 are separated by 48 nucleotides
AZOBR_RS01735: AZOBR_RS01735 - death-on-curing protein, at 355,464 to 355,907
_RS01735
AZOBR_RS01740: AZOBR_RS01740 - AbrB family transcriptional regulator, at 355,914 to 356,138
_RS01740
AZOBR_RS01745: AZOBR_RS01745 - membrane protein, at 356,238 to 356,666
_RS01745
AZOBR_RS01750: AZOBR_RS01750 - uroporphyrinogen decarboxylase, at 356,715 to 357,761
_RS01750
Position (kb) 355
356
357 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 355.311 kb on + strand at 355.312 kb on - strand at 355.312 kb on - strand at 355.466 kb on - strand at 355.504 kb on + strand at 355.504 kb on + strand at 355.505 kb on - strand at 355.505 kb on - strand at 355.505 kb on - strand at 355.744 kb on + strand, within AZOBR_RS01735 at 355.745 kb on - strand, within AZOBR_RS01735 at 355.759 kb on + strand, within AZOBR_RS01735 at 355.877 kb on - strand at 355.916 kb on - strand at 355.987 kb on + strand, within AZOBR_RS01740 at 356.171 kb on - strand at 356.171 kb on - strand at 356.183 kb on + strand at 356.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 355,311 + -2.4 355,312 - -3.3 355,312 - +1.0 355,466 - -3.3 355,504 + +0.0 355,504 + -0.8 355,505 - -0.3 355,505 - -1.8 355,505 - +0.2 355,744 + AZOBR_RS01735 0.63 +0.0 355,745 - AZOBR_RS01735 0.63 +2.3 355,759 + AZOBR_RS01735 0.66 +0.4 355,877 - -1.0 355,916 - +0.6 355,987 + AZOBR_RS01740 0.32 -4.0 356,171 - -1.4 356,171 - -3.4 356,183 + +0.4 356,232 - +0.7
Or see this region's nucleotide sequence