Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01705 and AZOBR_RS01710 are separated by 13 nucleotides AZOBR_RS01710 and AZOBR_RS01715 are separated by 242 nucleotides AZOBR_RS01715 and AZOBR_RS01720 are separated by 217 nucleotides AZOBR_RS01720 and AZOBR_RS01725 are separated by 140 nucleotides
AZOBR_RS01705: AZOBR_RS01705 - murein transglycosylase, at 347,605 to 348,810
_RS01705
AZOBR_RS01710: AZOBR_RS01710 - translocase, at 348,824 to 349,498
_RS01710
AZOBR_RS01715: AZOBR_RS01715 - exlusion protein FxsA, at 349,741 to 350,241
_RS01715
AZOBR_RS01720: AZOBR_RS01720 - preprotein translocase subunit SecB, at 350,459 to 350,956
_RS01720
AZOBR_RS01725: AZOBR_RS01725 - transcription termination factor Rho, at 351,097 to 352,353
_RS01725
Position (kb) 349
350
351 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 349.219 kb on - strand, within AZOBR_RS01710 at 349.559 kb on + strand at 349.559 kb on + strand at 349.585 kb on + strand at 349.585 kb on + strand at 349.585 kb on + strand at 349.586 kb on - strand at 349.586 kb on - strand at 349.586 kb on - strand at 349.586 kb on - strand at 349.704 kb on + strand at 349.705 kb on - strand at 349.705 kb on - strand at 349.705 kb on - strand at 349.708 kb on + strand at 350.082 kb on + strand, within AZOBR_RS01715 at 350.083 kb on - strand, within AZOBR_RS01715 at 350.083 kb on - strand, within AZOBR_RS01715 at 350.222 kb on - strand at 350.399 kb on + strand at 350.400 kb on - strand at 350.531 kb on + strand, within AZOBR_RS01720 at 350.531 kb on + strand, within AZOBR_RS01720 at 350.532 kb on - strand, within AZOBR_RS01720 at 350.651 kb on + strand, within AZOBR_RS01720 at 350.651 kb on + strand, within AZOBR_RS01720 at 350.652 kb on - strand, within AZOBR_RS01720 at 350.724 kb on - strand, within AZOBR_RS01720 at 351.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 349,219 - AZOBR_RS01710 0.59 -2.2 349,559 + +1.4 349,559 + -0.2 349,585 + -0.2 349,585 + -0.0 349,585 + +0.8 349,586 - -5.9 349,586 - -4.0 349,586 - +0.4 349,586 - +0.6 349,704 + -1.2 349,705 - -0.8 349,705 - +1.0 349,705 - -3.4 349,708 + +0.1 350,082 + AZOBR_RS01715 0.68 +1.0 350,083 - AZOBR_RS01715 0.68 -1.0 350,083 - AZOBR_RS01715 0.68 -2.1 350,222 - -0.4 350,399 + +1.6 350,400 - +0.1 350,531 + AZOBR_RS01720 0.14 +2.1 350,531 + AZOBR_RS01720 0.14 +2.7 350,532 - AZOBR_RS01720 0.15 -0.3 350,651 + AZOBR_RS01720 0.39 -1.4 350,651 + AZOBR_RS01720 0.39 +2.1 350,652 - AZOBR_RS01720 0.39 +1.7 350,724 - AZOBR_RS01720 0.53 +0.6 351,099 - +0.8
Or see this region's nucleotide sequence