Experiment: Sisomicin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01565 and AZOBR_RS01570 are separated by 82 nucleotides AZOBR_RS01570 and AZOBR_RS01575 are separated by 164 nucleotides AZOBR_RS01575 and AZOBR_RS01580 are separated by 19 nucleotides AZOBR_RS01580 and AZOBR_RS01585 are separated by 71 nucleotides
AZOBR_RS01565: AZOBR_RS01565 - endonuclease III, at 321,932 to 322,579
_RS01565
AZOBR_RS01570: AZOBR_RS01570 - hypothetical protein, at 322,662 to 323,180
_RS01570
AZOBR_RS01575: AZOBR_RS01575 - 6-O-methylguanine DNA methyltransferase, at 323,345 to 324,169
_RS01575
AZOBR_RS01580: AZOBR_RS01580 - dihydrodipicolinate reductase, at 324,189 to 324,986
_RS01580
AZOBR_RS01585: AZOBR_RS01585 - zinc transporter, at 325,058 to 326,044
_RS01585
Position (kb) 323
324
325 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 322.357 kb on - strand, within AZOBR_RS01565 at 322.491 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.503 kb on + strand, within AZOBR_RS01565 at 322.504 kb on - strand, within AZOBR_RS01565 at 322.504 kb on - strand, within AZOBR_RS01565 at 322.905 kb on + strand, within AZOBR_RS01570 at 322.906 kb on - strand, within AZOBR_RS01570 at 323.609 kb on + strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.01 mg/ml remove 322,357 - AZOBR_RS01565 0.66 -1.2 322,491 + AZOBR_RS01565 0.86 -1.6 322,503 + AZOBR_RS01565 0.88 -1.1 322,503 + AZOBR_RS01565 0.88 -4.3 322,503 + AZOBR_RS01565 0.88 -3.2 322,504 - AZOBR_RS01565 0.88 -1.4 322,504 - AZOBR_RS01565 0.88 -0.0 322,905 + AZOBR_RS01570 0.47 +1.3 322,906 - AZOBR_RS01570 0.47 +2.1 323,609 + AZOBR_RS01575 0.32 -1.6 323,610 - AZOBR_RS01575 0.32 -0.0 323,610 - AZOBR_RS01575 0.32 +1.1 323,610 - AZOBR_RS01575 0.32 -0.2 324,086 + AZOBR_RS01575 0.90 -0.0 324,086 + AZOBR_RS01575 0.90 -0.0 324,167 + -3.3
Or see this region's nucleotide sequence