Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS20665

Experiment: soil EcoFAB sample 1; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS20655 and ABZR87_RS20660 overlap by 8 nucleotidesABZR87_RS20660 and ABZR87_RS20665 are separated by 2 nucleotidesABZR87_RS20665 and ABZR87_RS20670 are separated by 12 nucleotidesABZR87_RS20670 and ABZR87_RS20675 are separated by 4 nucleotides ABZR87_RS20655: ABZR87_RS20655 - thiamine pyrophosphate-binding protein, at 1,022,205 to 1,023,932 _RS20655 ABZR87_RS20660: ABZR87_RS20660 - SDR family oxidoreductase, at 1,023,925 to 1,024,728 _RS20660 ABZR87_RS20665: ABZR87_RS20665 - IclR family transcriptional regulator, at 1,024,731 to 1,025,558 _RS20665 ABZR87_RS20670: ABZR87_RS20670 - alpha/beta hydrolase, at 1,025,571 to 1,026,473 _RS20670 ABZR87_RS20675: ABZR87_RS20675 - cupin domain-containing protein, at 1,026,478 to 1,027,014 _RS20675 Position (kb) 1024 1025 1026Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1023.927 kb on - strandat 1023.954 kb on + strandat 1023.954 kb on + strandat 1023.955 kb on - strandat 1023.955 kb on - strandat 1023.977 kb on + strandat 1024.693 kb on + strandat 1024.694 kb on - strandat 1024.786 kb on + strandat 1024.786 kb on + strandat 1024.964 kb on - strand, within ABZR87_RS20665at 1024.964 kb on - strand, within ABZR87_RS20665at 1024.964 kb on - strand, within ABZR87_RS20665at 1025.057 kb on - strand, within ABZR87_RS20665at 1025.188 kb on + strand, within ABZR87_RS20665at 1025.300 kb on - strand, within ABZR87_RS20665at 1025.518 kb on + strandat 1025.519 kb on - strandat 1025.519 kb on - strandat 1025.558 kb on + strandat 1025.558 kb on + strandat 1025.558 kb on + strandat 1025.559 kb on - strandat 1025.559 kb on - strandat 1025.639 kb on + strandat 1025.639 kb on + strandat 1025.639 kb on + strandat 1025.640 kb on - strandat 1025.640 kb on - strandat 1025.641 kb on + strandat 1025.641 kb on + strandat 1025.642 kb on - strandat 1025.642 kb on - strandat 1025.724 kb on - strand, within ABZR87_RS20670at 1025.785 kb on + strand, within ABZR87_RS20670at 1025.785 kb on + strand, within ABZR87_RS20670at 1025.785 kb on + strand, within ABZR87_RS20670at 1025.786 kb on - strand, within ABZR87_RS20670at 1026.079 kb on + strand, within ABZR87_RS20670at 1026.080 kb on - strand, within ABZR87_RS20670at 1026.080 kb on - strand, within ABZR87_RS20670at 1026.173 kb on - strand, within ABZR87_RS20670at 1026.221 kb on - strand, within ABZR87_RS20670at 1026.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction soil EcoFAB sample 1; outgrowth in LB
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1,023,927 - -2.1
1,023,954 + +2.2
1,023,954 + -2.6
1,023,955 - +0.1
1,023,955 - -2.7
1,023,977 + +0.2
1,024,693 + +0.2
1,024,694 - +2.1
1,024,786 + -0.8
1,024,786 + -0.5
1,024,964 - ABZR87_RS20665 0.28 -0.6
1,024,964 - ABZR87_RS20665 0.28 -3.3
1,024,964 - ABZR87_RS20665 0.28 -2.2
1,025,057 - ABZR87_RS20665 0.39 -1.8
1,025,188 + ABZR87_RS20665 0.55 -0.6
1,025,300 - ABZR87_RS20665 0.69 -3.0
1,025,518 + -0.4
1,025,519 - -3.4
1,025,519 - -2.1
1,025,558 + -0.7
1,025,558 + -2.1
1,025,558 + -2.3
1,025,559 - -2.7
1,025,559 - -0.7
1,025,639 + +0.6
1,025,639 + +0.2
1,025,639 + +1.9
1,025,640 - +1.5
1,025,640 - +0.8
1,025,641 + +0.1
1,025,641 + +0.6
1,025,642 - -2.6
1,025,642 - -0.2
1,025,724 - ABZR87_RS20670 0.17 -0.8
1,025,785 + ABZR87_RS20670 0.24 -1.2
1,025,785 + ABZR87_RS20670 0.24 -3.9
1,025,785 + ABZR87_RS20670 0.24 +0.6
1,025,786 - ABZR87_RS20670 0.24 -3.7
1,026,079 + ABZR87_RS20670 0.56 +0.7
1,026,080 - ABZR87_RS20670 0.56 -1.1
1,026,080 - ABZR87_RS20670 0.56 +1.7
1,026,173 - ABZR87_RS20670 0.67 -3.7
1,026,221 - ABZR87_RS20670 0.72 +0.6
1,026,516 - -0.4

Or see this region's nucleotide sequence