Experiment: soil EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16730 and ABZR87_RS16735 overlap by 4 nucleotides ABZR87_RS16735 and ABZR87_RS16740 are separated by 276 nucleotides
ABZR87_RS16730: ABZR87_RS16730 - 2-methylaconitate cis-trans isomerase PrpF, at 101,854 to 103,038
_RS16730
ABZR87_RS16735: ABZR87_RS16735 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 103,035 to 105,632
_RS16735
ABZR87_RS16740: ABZR87_RS16740 - 2-methylcitrate synthase, at 105,909 to 107,078
_RS16740
Position (kb) 103
104
105
106 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.345 kb on - strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.103 kb on - strand at 103.103 kb on - strand at 103.408 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.792 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.835 kb on - strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.890 kb on + strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735 at 104.048 kb on - strand, within ABZR87_RS16735 at 104.146 kb on + strand, within ABZR87_RS16735 at 104.147 kb on - strand, within ABZR87_RS16735 at 104.147 kb on - strand, within ABZR87_RS16735 at 104.284 kb on + strand, within ABZR87_RS16735 at 104.593 kb on + strand, within ABZR87_RS16735 at 104.641 kb on + strand, within ABZR87_RS16735 at 104.641 kb on + strand, within ABZR87_RS16735 at 104.641 kb on + strand, within ABZR87_RS16735 at 104.641 kb on + strand, within ABZR87_RS16735 at 104.641 kb on + strand, within ABZR87_RS16735 at 104.642 kb on - strand, within ABZR87_RS16735 at 104.642 kb on - strand, within ABZR87_RS16735 at 104.642 kb on - strand, within ABZR87_RS16735 at 104.692 kb on + strand, within ABZR87_RS16735 at 104.692 kb on + strand, within ABZR87_RS16735 at 104.807 kb on - strand, within ABZR87_RS16735 at 104.818 kb on + strand, within ABZR87_RS16735 at 105.262 kb on - strand, within ABZR87_RS16735 at 105.496 kb on - strand at 105.496 kb on - strand at 105.526 kb on + strand at 105.526 kb on + strand at 105.526 kb on + strand at 105.526 kb on + strand at 105.526 kb on + strand at 105.527 kb on - strand at 105.527 kb on - strand at 105.527 kb on - strand at 105.527 kb on - strand at 105.542 kb on - strand at 105.587 kb on - strand at 105.587 kb on - strand at 105.747 kb on + strand at 105.839 kb on + strand at 105.903 kb on + strand at 105.904 kb on - strand at 105.904 kb on - strand at 105.958 kb on + strand at 105.958 kb on + strand at 105.958 kb on + strand at 105.958 kb on + strand at 105.958 kb on + strand at 105.958 kb on + strand at 105.959 kb on - strand at 105.959 kb on - strand at 105.959 kb on - strand at 106.084 kb on + strand, within ABZR87_RS16740 at 106.085 kb on - strand, within ABZR87_RS16740 at 106.249 kb on + strand, within ABZR87_RS16740 at 106.250 kb on - strand, within ABZR87_RS16740 at 106.333 kb on + strand, within ABZR87_RS16740 at 106.420 kb on + strand, within ABZR87_RS16740 at 106.420 kb on + strand, within ABZR87_RS16740 at 106.420 kb on + strand, within ABZR87_RS16740 at 106.420 kb on + strand, within ABZR87_RS16740 at 106.421 kb on - strand, within ABZR87_RS16740 at 106.421 kb on - strand, within ABZR87_RS16740 at 106.489 kb on + strand, within ABZR87_RS16740 at 106.489 kb on + strand, within ABZR87_RS16740 at 106.621 kb on + strand, within ABZR87_RS16740 at 106.622 kb on - strand, within ABZR87_RS16740 at 106.622 kb on - strand, within ABZR87_RS16740 at 106.627 kb on + strand, within ABZR87_RS16740 at 106.627 kb on + strand, within ABZR87_RS16740
Per-strain Table
Position Strand Gene LocusTag Fraction soil EcoFAB sample 1; outgrowth in LB remove 102,173 + ABZR87_RS16730 0.27 +1.4 102,173 + ABZR87_RS16730 0.27 -2.9 102,173 + ABZR87_RS16730 0.27 -4.2 102,174 - ABZR87_RS16730 0.27 -0.2 102,174 - ABZR87_RS16730 0.27 -4.0 102,174 - ABZR87_RS16730 0.27 -2.1 102,345 - ABZR87_RS16730 0.41 -2.5 102,782 + ABZR87_RS16730 0.78 -3.2 102,782 + ABZR87_RS16730 0.78 -3.4 102,783 - ABZR87_RS16730 0.78 -1.5 102,783 - ABZR87_RS16730 0.78 +0.6 102,783 - ABZR87_RS16730 0.78 -2.0 102,783 - ABZR87_RS16730 0.78 -2.5 103,001 + -3.9 103,001 + -3.9 103,001 + -3.6 103,001 + -3.1 103,001 + -3.6 103,001 + -0.6 103,001 + -2.1 103,002 - -1.8 103,002 - -3.2 103,002 - -4.0 103,002 - -3.3 103,002 - -2.5 103,103 - -1.9 103,103 - -4.6 103,408 + ABZR87_RS16735 0.14 +0.2 103,453 + ABZR87_RS16735 0.16 -4.1 103,453 + ABZR87_RS16735 0.16 -1.6 103,453 + ABZR87_RS16735 0.16 -4.7 103,453 + ABZR87_RS16735 0.16 +0.1 103,454 - ABZR87_RS16735 0.16 -3.9 103,454 - ABZR87_RS16735 0.16 -2.7 103,454 - ABZR87_RS16735 0.16 -1.6 103,618 + ABZR87_RS16735 0.22 -2.5 103,618 + ABZR87_RS16735 0.22 -3.6 103,619 - ABZR87_RS16735 0.22 -2.8 103,619 - ABZR87_RS16735 0.22 -3.2 103,660 + ABZR87_RS16735 0.24 -3.0 103,660 + ABZR87_RS16735 0.24 -3.2 103,660 + ABZR87_RS16735 0.24 -4.3 103,660 + ABZR87_RS16735 0.24 -2.5 103,792 + ABZR87_RS16735 0.29 -0.2 103,810 + ABZR87_RS16735 0.30 -2.1 103,810 + ABZR87_RS16735 0.30 -3.8 103,810 + ABZR87_RS16735 0.30 +0.6 103,810 + ABZR87_RS16735 0.30 -1.6 103,810 + ABZR87_RS16735 0.30 -3.3 103,810 + ABZR87_RS16735 0.30 -5.4 103,810 + ABZR87_RS16735 0.30 -3.3 103,811 - ABZR87_RS16735 0.30 -2.5 103,811 - ABZR87_RS16735 0.30 -2.0 103,811 - ABZR87_RS16735 0.30 -2.5 103,811 - ABZR87_RS16735 0.30 -3.5 103,811 - ABZR87_RS16735 0.30 -2.3 103,811 - ABZR87_RS16735 0.30 -2.8 103,835 - ABZR87_RS16735 0.31 -4.9 103,888 + ABZR87_RS16735 0.33 -3.3 103,888 + ABZR87_RS16735 0.33 -3.7 103,888 + ABZR87_RS16735 0.33 -4.6 103,888 + ABZR87_RS16735 0.33 -3.5 103,888 + ABZR87_RS16735 0.33 -3.9 103,888 + ABZR87_RS16735 0.33 -2.3 103,888 + ABZR87_RS16735 0.33 -3.6 103,888 + ABZR87_RS16735 0.33 -4.0 103,889 - ABZR87_RS16735 0.33 -3.9 103,889 - ABZR87_RS16735 0.33 -2.2 103,889 - ABZR87_RS16735 0.33 -3.0 103,889 - ABZR87_RS16735 0.33 -3.0 103,889 - ABZR87_RS16735 0.33 -3.3 103,889 - ABZR87_RS16735 0.33 -3.8 103,889 - ABZR87_RS16735 0.33 -3.3 103,890 + ABZR87_RS16735 0.33 -3.0 103,891 - ABZR87_RS16735 0.33 -4.4 103,891 - ABZR87_RS16735 0.33 +0.8 104,048 - ABZR87_RS16735 0.39 +0.6 104,146 + ABZR87_RS16735 0.43 -2.1 104,147 - ABZR87_RS16735 0.43 -2.7 104,147 - ABZR87_RS16735 0.43 -4.3 104,284 + ABZR87_RS16735 0.48 -2.4 104,593 + ABZR87_RS16735 0.60 -1.8 104,641 + ABZR87_RS16735 0.62 -4.4 104,641 + ABZR87_RS16735 0.62 -3.6 104,641 + ABZR87_RS16735 0.62 -2.8 104,641 + ABZR87_RS16735 0.62 -4.1 104,641 + ABZR87_RS16735 0.62 -3.8 104,642 - ABZR87_RS16735 0.62 -1.2 104,642 - ABZR87_RS16735 0.62 -3.9 104,642 - ABZR87_RS16735 0.62 -4.5 104,692 + ABZR87_RS16735 0.64 -2.5 104,692 + ABZR87_RS16735 0.64 -2.8 104,807 - ABZR87_RS16735 0.68 -6.0 104,818 + ABZR87_RS16735 0.69 -0.9 105,262 - ABZR87_RS16735 0.86 -4.0 105,496 - -3.3 105,496 - -3.0 105,526 + -3.3 105,526 + -3.7 105,526 + -3.7 105,526 + +0.2 105,526 + -2.6 105,527 - -2.6 105,527 - -2.4 105,527 - -3.2 105,527 - -4.2 105,542 - -4.6 105,587 - -3.4 105,587 - -1.8 105,747 + -0.9 105,839 + -1.8 105,903 + -4.1 105,904 - -2.3 105,904 - -0.5 105,958 + -3.5 105,958 + -2.7 105,958 + -3.4 105,958 + -3.3 105,958 + -2.0 105,958 + +0.2 105,959 - -1.3 105,959 - +0.8 105,959 - -0.8 106,084 + ABZR87_RS16740 0.15 -3.8 106,085 - ABZR87_RS16740 0.15 -3.3 106,249 + ABZR87_RS16740 0.29 -2.1 106,250 - ABZR87_RS16740 0.29 -1.3 106,333 + ABZR87_RS16740 0.36 -2.4 106,420 + ABZR87_RS16740 0.44 -3.7 106,420 + ABZR87_RS16740 0.44 -3.3 106,420 + ABZR87_RS16740 0.44 -5.0 106,420 + ABZR87_RS16740 0.44 -3.3 106,421 - ABZR87_RS16740 0.44 -1.9 106,421 - ABZR87_RS16740 0.44 -0.8 106,489 + ABZR87_RS16740 0.50 -0.3 106,489 + ABZR87_RS16740 0.50 -3.4 106,621 + ABZR87_RS16740 0.61 +0.7 106,622 - ABZR87_RS16740 0.61 -3.1 106,622 - ABZR87_RS16740 0.61 -2.9 106,627 + ABZR87_RS16740 0.61 -1.5 106,627 + ABZR87_RS16740 0.61 -3.8
Or see this region's nucleotide sequence