Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4026 and BT4027 are separated by 26 nucleotides BT4027 and BT4028 overlap by 4 nucleotides BT4028 and BT4029 are separated by 143 nucleotides BT4029 and BT4030 are separated by 125 nucleotides BT4030 and BT4031 are separated by 151 nucleotides
BT4026: BT4026 - conserved hypothetical protein (NCBI ptt file), at 5,241,062 to 5,242,060
BT4026
BT4027: BT4027 - hypothetical protein (NCBI ptt file), at 5,242,087 to 5,242,314
BT4027
BT4028: BT4028 - hypothetical protein (NCBI ptt file), at 5,242,311 to 5,242,583
BT4028
BT4029: BT4029 - hypothetical protein (NCBI ptt file), at 5,242,727 to 5,243,467
BT4029
BT4030: BT4030 - hypothetical protein (NCBI ptt file), at 5,243,593 to 5,243,847
BT4030
BT4031: BT4031 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 5,243,999 to 5,244,502
BT4031
Position (kb) 5242
5243
5244 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5241.737 kb on - strand, within BT4026 at 5241.886 kb on - strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.978 kb on + strand at 5242.098 kb on + strand at 5242.117 kb on + strand, within BT4027 at 5242.118 kb on - strand, within BT4027 at 5242.138 kb on - strand, within BT4027 at 5242.139 kb on + strand, within BT4027 at 5242.140 kb on - strand, within BT4027 at 5242.423 kb on - strand, within BT4028 at 5242.582 kb on - strand at 5242.615 kb on + strand at 5242.739 kb on - strand at 5242.760 kb on - strand at 5242.821 kb on - strand, within BT4029 at 5243.118 kb on + strand, within BT4029 at 5243.121 kb on + strand, within BT4029 at 5243.157 kb on + strand, within BT4029 at 5243.164 kb on - strand, within BT4029 at 5243.272 kb on - strand, within BT4029 at 5243.500 kb on - strand at 5243.508 kb on + strand at 5243.508 kb on + strand at 5243.508 kb on + strand at 5243.509 kb on - strand at 5243.509 kb on - strand at 5243.510 kb on + strand at 5243.510 kb on + strand at 5243.510 kb on + strand at 5243.511 kb on - strand at 5243.517 kb on - strand at 5243.517 kb on - strand at 5243.519 kb on - strand at 5243.523 kb on - strand at 5243.544 kb on + strand at 5243.544 kb on + strand at 5243.551 kb on + strand at 5243.582 kb on + strand at 5243.597 kb on - strand at 5243.597 kb on - strand at 5243.598 kb on + strand at 5243.599 kb on - strand at 5243.606 kb on + strand at 5243.623 kb on + strand, within BT4030 at 5243.678 kb on - strand, within BT4030 at 5243.691 kb on - strand, within BT4030 at 5243.719 kb on - strand, within BT4030 at 5243.742 kb on - strand, within BT4030 at 5243.747 kb on + strand, within BT4030 at 5243.774 kb on - strand, within BT4030 at 5243.774 kb on - strand, within BT4030 at 5243.774 kb on - strand, within BT4030 at 5243.790 kb on + strand, within BT4030 at 5243.791 kb on - strand, within BT4030 at 5243.818 kb on - strand, within BT4030 at 5243.820 kb on + strand, within BT4030 at 5243.948 kb on + strand at 5243.974 kb on + strand at 5244.131 kb on + strand, within BT4031 at 5244.131 kb on + strand, within BT4031 at 5244.132 kb on - strand, within BT4031 at 5244.172 kb on + strand, within BT4031 at 5244.271 kb on + strand, within BT4031
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 5,241,737 - BT4026 0.68 +0.6 5,241,886 - BT4026 0.82 +0.1 5,241,931 + BT4026 0.87 -0.2 5,241,931 + BT4026 0.87 -0.4 5,241,978 + -0.9 5,242,098 + +0.5 5,242,117 + BT4027 0.13 -1.3 5,242,118 - BT4027 0.14 -0.4 5,242,138 - BT4027 0.22 -1.3 5,242,139 + BT4027 0.23 -0.8 5,242,140 - BT4027 0.23 +0.5 5,242,423 - BT4028 0.41 -0.1 5,242,582 - +1.0 5,242,615 + -1.5 5,242,739 - -2.1 5,242,760 - -0.4 5,242,821 - BT4029 0.13 +0.0 5,243,118 + BT4029 0.53 -2.0 5,243,121 + BT4029 0.53 -1.0 5,243,157 + BT4029 0.58 -2.3 5,243,164 - BT4029 0.59 +0.5 5,243,272 - BT4029 0.74 -1.0 5,243,500 - -1.3 5,243,508 + -0.5 5,243,508 + +0.5 5,243,508 + +0.5 5,243,509 - +1.1 5,243,509 - -0.5 5,243,510 + +0.1 5,243,510 + +0.8 5,243,510 + -2.5 5,243,511 - +1.0 5,243,517 - -2.0 5,243,517 - -0.3 5,243,519 - -0.9 5,243,523 - -0.1 5,243,544 + +1.5 5,243,544 + -1.8 5,243,551 + +0.2 5,243,582 + -2.4 5,243,597 - -1.3 5,243,597 - -1.4 5,243,598 + +0.0 5,243,599 - +0.1 5,243,606 + -3.0 5,243,623 + BT4030 0.12 +0.0 5,243,678 - BT4030 0.33 -1.5 5,243,691 - BT4030 0.38 -0.3 5,243,719 - BT4030 0.49 -0.4 5,243,742 - BT4030 0.58 -0.0 5,243,747 + BT4030 0.60 -1.8 5,243,774 - BT4030 0.71 -1.2 5,243,774 - BT4030 0.71 -1.5 5,243,774 - BT4030 0.71 +2.2 5,243,790 + BT4030 0.77 -3.4 5,243,791 - BT4030 0.78 -0.2 5,243,818 - BT4030 0.88 -2.4 5,243,820 + BT4030 0.89 +0.4 5,243,948 + +0.2 5,243,974 + -0.4 5,244,131 + BT4031 0.26 -1.2 5,244,131 + BT4031 0.26 +0.0 5,244,132 - BT4031 0.26 -2.0 5,244,172 + BT4031 0.34 +0.7 5,244,271 + BT4031 0.54 +0.1
Or see this region's nucleotide sequence