Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3945 and BT3946 are separated by 52 nucleotides BT3946 and BT3947 are separated by 8 nucleotides BT3947 and BT3948 are separated by 45 nucleotides
BT3945: BT3945 - methionyl-tRNA formyltransferase (NCBI ptt file), at 5,120,937 to 5,121,905
BT3945
BT3946: BT3946 - ribulose-phosphate 3-epimerase (NCBI ptt file), at 5,121,958 to 5,122,608
BT3946
BT3947: BT3947 - competence protein (NCBI ptt file), at 5,122,617 to 5,124,719
BT3947
BT3948: BT3948 - conserved hypothetical protein, putative exopolyphosphatase-related protein (NCBI ptt file), at 5,124,765 to 5,125,802
BT3948
Position (kb)
5122
5123
5124
5125 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5121.951 kb on + strand at 5121.951 kb on + strand at 5121.951 kb on + strand at 5122.633 kb on + strand at 5122.641 kb on + strand at 5122.647 kb on + strand at 5122.647 kb on + strand at 5122.647 kb on + strand at 5122.647 kb on + strand at 5122.650 kb on - strand at 5122.730 kb on + strand at 5122.774 kb on - strand at 5122.814 kb on - strand at 5122.961 kb on + strand, within BT3947 at 5122.973 kb on + strand, within BT3947 at 5122.974 kb on - strand, within BT3947 at 5122.999 kb on + strand, within BT3947 at 5123.029 kb on - strand, within BT3947 at 5123.081 kb on - strand, within BT3947 at 5123.087 kb on - strand, within BT3947 at 5123.087 kb on - strand, within BT3947 at 5123.245 kb on - strand, within BT3947 at 5123.245 kb on - strand, within BT3947 at 5123.470 kb on + strand, within BT3947 at 5123.470 kb on - strand, within BT3947 at 5123.471 kb on - strand, within BT3947 at 5123.567 kb on - strand, within BT3947 at 5123.583 kb on - strand, within BT3947 at 5123.640 kb on - strand, within BT3947 at 5123.725 kb on + strand, within BT3947 at 5123.758 kb on - strand at 5123.793 kb on + strand, within BT3947 at 5123.793 kb on + strand, within BT3947 at 5123.816 kb on - strand, within BT3947 at 5123.860 kb on - strand, within BT3947 at 5123.895 kb on - strand, within BT3947 at 5123.899 kb on + strand, within BT3947 at 5123.903 kb on - strand, within BT3947 at 5123.909 kb on - strand, within BT3947 at 5124.105 kb on + strand, within BT3947 at 5124.105 kb on + strand, within BT3947 at 5124.105 kb on + strand, within BT3947 at 5124.249 kb on + strand, within BT3947 at 5124.282 kb on - strand, within BT3947 at 5124.333 kb on - strand, within BT3947 at 5124.395 kb on - strand, within BT3947 at 5124.474 kb on - strand, within BT3947 at 5124.525 kb on - strand at 5124.525 kb on - strand at 5124.532 kb on - strand at 5124.537 kb on + strand at 5124.541 kb on + strand at 5124.542 kb on - strand at 5124.553 kb on - strand at 5124.554 kb on + strand at 5124.656 kb on + strand at 5124.678 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 5,121,951 + -1.6 5,121,951 + -0.4 5,121,951 + +0.3 5,122,633 + -0.3 5,122,641 + -0.4 5,122,647 + -0.0 5,122,647 + -2.4 5,122,647 + -1.9 5,122,647 + -2.1 5,122,650 - -0.6 5,122,730 + -0.3 5,122,774 - -0.4 5,122,814 - -1.7 5,122,961 + BT3947 0.16 +1.7 5,122,973 + BT3947 0.17 -1.0 5,122,974 - BT3947 0.17 +1.1 5,122,999 + BT3947 0.18 +1.1 5,123,029 - BT3947 0.20 +1.5 5,123,081 - BT3947 0.22 -0.8 5,123,087 - BT3947 0.22 -1.4 5,123,087 - BT3947 0.22 +2.3 5,123,245 - BT3947 0.30 +0.4 5,123,245 - BT3947 0.30 -0.5 5,123,470 + BT3947 0.41 -1.8 5,123,470 - BT3947 0.41 +0.2 5,123,471 - BT3947 0.41 +3.2 5,123,567 - BT3947 0.45 -1.0 5,123,583 - BT3947 0.46 +1.3 5,123,640 - BT3947 0.49 +0.2 5,123,725 + BT3947 0.53 +1.6 5,123,758 - +1.1 5,123,793 + BT3947 0.56 -0.9 5,123,793 + BT3947 0.56 +0.3 5,123,816 - BT3947 0.57 +0.9 5,123,860 - BT3947 0.59 -1.0 5,123,895 - BT3947 0.61 +0.6 5,123,899 + BT3947 0.61 +1.1 5,123,903 - BT3947 0.61 +1.1 5,123,909 - BT3947 0.61 +0.3 5,124,105 + BT3947 0.71 -0.5 5,124,105 + BT3947 0.71 +1.1 5,124,105 + BT3947 0.71 -1.4 5,124,249 + BT3947 0.78 -0.5 5,124,282 - BT3947 0.79 -0.5 5,124,333 - BT3947 0.82 +0.5 5,124,395 - BT3947 0.85 -0.2 5,124,474 - BT3947 0.88 +1.6 5,124,525 - -0.8 5,124,525 - -2.6 5,124,532 - +1.2 5,124,537 + +1.1 5,124,541 + -0.8 5,124,542 - +2.4 5,124,553 - -1.1 5,124,554 + -0.9 5,124,656 + -2.3 5,124,678 - -0.7
Or see this region's nucleotide sequence