Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1492 and BT1493 are separated by 109 nucleotides BT1493 and BT1494 are separated by 8 nucleotides BT1494 and BT1495 are separated by 72 nucleotides BT1495 and BT1496 are separated by 98 nucleotides
BT1492: BT1492 - tyrosine phenol-lyase (NCBI ptt file), at 1,841,886 to 1,843,265
BT1492
BT1493: BT1493 - conserved hypothetical protein (NCBI ptt file), at 1,843,375 to 1,843,677
BT1493
BT1494: BT1494 - conserved hypothetical protein (NCBI ptt file), at 1,843,686 to 1,843,910
BT1494
BT1495: BT1495 - siderophore (surfactin) biosynthesis regulatory protein (NCBI ptt file), at 1,843,983 to 1,844,588
BT1495
BT1496: BT1496 - hemolysin-related protein, containing CBS domain (NCBI ptt file), at 1,844,687 to 1,845,745
BT1496
Position (kb)
1843
1844 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1842.708 kb on + strand, within BT1492 at 1842.708 kb on + strand, within BT1492 at 1842.709 kb on - strand, within BT1492 at 1842.748 kb on - strand, within BT1492 at 1842.748 kb on - strand, within BT1492 at 1842.751 kb on + strand, within BT1492 at 1842.840 kb on - strand, within BT1492 at 1842.850 kb on + strand, within BT1492 at 1842.855 kb on + strand, within BT1492 at 1842.856 kb on - strand, within BT1492 at 1843.013 kb on - strand, within BT1492 at 1843.032 kb on + strand, within BT1492 at 1843.033 kb on - strand, within BT1492 at 1843.033 kb on - strand, within BT1492 at 1843.091 kb on - strand, within BT1492 at 1843.121 kb on + strand, within BT1492 at 1843.121 kb on + strand, within BT1492 at 1843.122 kb on - strand, within BT1492 at 1843.271 kb on - strand at 1843.298 kb on - strand at 1843.344 kb on + strand at 1843.368 kb on + strand at 1843.368 kb on + strand at 1843.369 kb on - strand at 1843.369 kb on - strand at 1843.390 kb on - strand at 1843.459 kb on + strand, within BT1493 at 1843.459 kb on + strand, within BT1493 at 1843.459 kb on + strand, within BT1493 at 1843.494 kb on - strand, within BT1493 at 1843.610 kb on + strand, within BT1493 at 1843.618 kb on + strand, within BT1493 at 1843.619 kb on - strand, within BT1493 at 1843.627 kb on - strand, within BT1493 at 1843.653 kb on - strand at 1843.672 kb on - strand at 1843.676 kb on - strand at 1843.682 kb on - strand at 1843.682 kb on - strand at 1843.721 kb on - strand, within BT1494 at 1843.768 kb on + strand, within BT1494 at 1843.788 kb on + strand, within BT1494 at 1843.830 kb on - strand, within BT1494 at 1843.843 kb on + strand, within BT1494 at 1843.843 kb on + strand, within BT1494 at 1843.905 kb on - strand at 1843.918 kb on + strand at 1843.953 kb on - strand at 1843.964 kb on - strand at 1843.969 kb on + strand at 1843.969 kb on + strand at 1843.992 kb on - strand at 1844.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 1,842,708 + BT1492 0.60 +2.4 1,842,708 + BT1492 0.60 -0.4 1,842,709 - BT1492 0.60 -0.5 1,842,748 - BT1492 0.62 -2.0 1,842,748 - BT1492 0.62 +1.9 1,842,751 + BT1492 0.63 +0.2 1,842,840 - BT1492 0.69 +1.2 1,842,850 + BT1492 0.70 -1.4 1,842,855 + BT1492 0.70 -1.5 1,842,856 - BT1492 0.70 -1.8 1,843,013 - BT1492 0.82 -1.2 1,843,032 + BT1492 0.83 +1.1 1,843,033 - BT1492 0.83 +0.4 1,843,033 - BT1492 0.83 -0.8 1,843,091 - BT1492 0.87 -1.4 1,843,121 + BT1492 0.89 -0.1 1,843,121 + BT1492 0.89 +1.8 1,843,122 - BT1492 0.90 -0.6 1,843,271 - +2.2 1,843,298 - -1.5 1,843,344 + -1.9 1,843,368 + -2.6 1,843,368 + -3.0 1,843,369 - +0.2 1,843,369 - -0.8 1,843,390 - +0.9 1,843,459 + BT1493 0.28 -0.2 1,843,459 + BT1493 0.28 -3.0 1,843,459 + BT1493 0.28 -0.1 1,843,494 - BT1493 0.39 -1.1 1,843,610 + BT1493 0.78 -1.4 1,843,618 + BT1493 0.80 +0.5 1,843,619 - BT1493 0.81 -0.9 1,843,627 - BT1493 0.83 -0.1 1,843,653 - -2.0 1,843,672 - -0.4 1,843,676 - -0.2 1,843,682 - -0.3 1,843,682 - +1.2 1,843,721 - BT1494 0.16 -0.6 1,843,768 + BT1494 0.36 +1.4 1,843,788 + BT1494 0.45 +0.2 1,843,830 - BT1494 0.64 +1.0 1,843,843 + BT1494 0.70 +1.2 1,843,843 + BT1494 0.70 +0.8 1,843,905 - -0.8 1,843,918 + -1.9 1,843,953 - -1.4 1,843,964 - -0.1 1,843,969 + +1.3 1,843,969 + -0.0 1,843,992 - -0.7 1,844,682 - +0.5
Or see this region's nucleotide sequence