Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1437 and BT1438 overlap by 113 nucleotides BT1438 and BT1439 are separated by 78 nucleotides
BT1437: BT1437 - conserved hypothetical protein (NCBI ptt file), at 1,766,896 to 1,768,029
BT1437
BT1438: BT1438 - putative membrane efflux protein (NCBI ptt file), at 1,767,917 to 1,769,335
BT1438
BT1439: BT1439 - Outer membrane binding protein for vancomycin (SusD-like) (from data) , at 1,769,414 to 1,770,895
BT1439
Position (kb)
1767
1768
1769
1770 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1766.917 kb on - strand at 1766.949 kb on + strand at 1767.000 kb on + strand at 1767.050 kb on - strand at 1767.115 kb on + strand, within BT1437 at 1767.214 kb on + strand, within BT1437 at 1767.214 kb on + strand, within BT1437 at 1767.445 kb on + strand, within BT1437 at 1767.446 kb on - strand, within BT1437 at 1767.508 kb on - strand, within BT1437 at 1767.589 kb on + strand, within BT1437 at 1767.590 kb on - strand, within BT1437 at 1767.595 kb on - strand, within BT1437 at 1767.598 kb on + strand, within BT1437 at 1767.674 kb on - strand, within BT1437 at 1767.693 kb on - strand, within BT1437 at 1767.731 kb on - strand, within BT1437 at 1767.848 kb on + strand, within BT1437 at 1767.869 kb on - strand, within BT1437 at 1767.869 kb on - strand, within BT1437 at 1768.012 kb on + strand at 1768.034 kb on + strand at 1768.056 kb on - strand at 1768.080 kb on + strand, within BT1438 at 1768.120 kb on - strand, within BT1438 at 1768.123 kb on + strand, within BT1438 at 1768.126 kb on + strand, within BT1438 at 1768.126 kb on + strand, within BT1438 at 1768.375 kb on - strand, within BT1438 at 1768.436 kb on + strand, within BT1438 at 1768.806 kb on + strand, within BT1438 at 1768.807 kb on - strand, within BT1438 at 1768.843 kb on + strand, within BT1438 at 1768.930 kb on + strand, within BT1438 at 1768.982 kb on - strand, within BT1438 at 1768.982 kb on - strand, within BT1438 at 1769.079 kb on + strand, within BT1438 at 1769.121 kb on - strand, within BT1438 at 1769.149 kb on - strand, within BT1438 at 1769.222 kb on - strand at 1769.255 kb on - strand at 1769.270 kb on - strand at 1769.366 kb on - strand at 1769.366 kb on - strand at 1769.423 kb on - strand at 1769.435 kb on - strand at 1769.458 kb on - strand at 1769.458 kb on - strand at 1769.458 kb on - strand at 1769.464 kb on - strand at 1769.585 kb on - strand, within BT1439 at 1769.624 kb on - strand, within BT1439 at 1769.630 kb on + strand, within BT1439 at 1769.631 kb on - strand, within BT1439 at 1769.631 kb on - strand, within BT1439 at 1769.640 kb on - strand, within BT1439 at 1769.728 kb on - strand, within BT1439 at 1769.732 kb on - strand, within BT1439 at 1769.735 kb on + strand, within BT1439 at 1769.735 kb on + strand, within BT1439 at 1769.864 kb on - strand, within BT1439 at 1769.893 kb on - strand, within BT1439 at 1769.902 kb on - strand, within BT1439 at 1769.902 kb on - strand, within BT1439 at 1769.956 kb on + strand, within BT1439 at 1769.987 kb on - strand, within BT1439 at 1769.991 kb on + strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1769.992 kb on - strand, within BT1439 at 1770.004 kb on - strand, within BT1439 at 1770.018 kb on + strand, within BT1439 at 1770.082 kb on + strand, within BT1439 at 1770.122 kb on - strand, within BT1439 at 1770.192 kb on - strand, within BT1439 at 1770.196 kb on - strand, within BT1439 at 1770.196 kb on - strand, within BT1439 at 1770.231 kb on + strand, within BT1439 at 1770.280 kb on - strand, within BT1439 at 1770.319 kb on - strand, within BT1439 at 1770.319 kb on - strand, within BT1439
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 1,766,917 - +0.8 1,766,949 + +0.9 1,767,000 + -1.4 1,767,050 - -0.2 1,767,115 + BT1437 0.19 +0.2 1,767,214 + BT1437 0.28 -1.4 1,767,214 + BT1437 0.28 -2.1 1,767,445 + BT1437 0.48 +1.0 1,767,446 - BT1437 0.49 -1.2 1,767,508 - BT1437 0.54 +1.6 1,767,589 + BT1437 0.61 -0.2 1,767,590 - BT1437 0.61 -3.2 1,767,595 - BT1437 0.62 -0.2 1,767,598 + BT1437 0.62 +0.8 1,767,674 - BT1437 0.69 -0.9 1,767,693 - BT1437 0.70 -0.6 1,767,731 - BT1437 0.74 +0.8 1,767,848 + BT1437 0.84 +0.8 1,767,869 - BT1437 0.86 -1.0 1,767,869 - BT1437 0.86 +0.3 1,768,012 + +0.6 1,768,034 + -3.2 1,768,056 - -1.4 1,768,080 + BT1438 0.11 -0.7 1,768,120 - BT1438 0.14 -3.8 1,768,123 + BT1438 0.15 -0.7 1,768,126 + BT1438 0.15 -2.2 1,768,126 + BT1438 0.15 -0.2 1,768,375 - BT1438 0.32 -1.7 1,768,436 + BT1438 0.37 -4.2 1,768,806 + BT1438 0.63 +0.4 1,768,807 - BT1438 0.63 -2.1 1,768,843 + BT1438 0.65 +0.5 1,768,930 + BT1438 0.71 -2.4 1,768,982 - BT1438 0.75 -0.6 1,768,982 - BT1438 0.75 -1.7 1,769,079 + BT1438 0.82 -0.2 1,769,121 - BT1438 0.85 -2.3 1,769,149 - BT1438 0.87 -0.1 1,769,222 - +0.8 1,769,255 - +0.8 1,769,270 - -0.6 1,769,366 - +1.4 1,769,366 - -2.7 1,769,423 - -0.8 1,769,435 - -0.2 1,769,458 - -0.0 1,769,458 - +1.0 1,769,458 - -0.2 1,769,464 - +1.0 1,769,585 - BT1439 0.12 -0.2 1,769,624 - BT1439 0.14 -0.5 1,769,630 + BT1439 0.15 +1.8 1,769,631 - BT1439 0.15 +1.3 1,769,631 - BT1439 0.15 +0.5 1,769,640 - BT1439 0.15 -0.8 1,769,728 - BT1439 0.21 -0.7 1,769,732 - BT1439 0.21 -1.5 1,769,735 + BT1439 0.22 -2.1 1,769,735 + BT1439 0.22 +0.0 1,769,864 - BT1439 0.30 -1.0 1,769,893 - BT1439 0.32 -1.5 1,769,902 - BT1439 0.33 -2.6 1,769,902 - BT1439 0.33 -1.6 1,769,956 + BT1439 0.37 +0.8 1,769,987 - BT1439 0.39 -0.5 1,769,991 + BT1439 0.39 +0.8 1,769,992 - BT1439 0.39 +0.4 1,769,992 - BT1439 0.39 -0.6 1,769,992 - BT1439 0.39 -0.7 1,770,004 - BT1439 0.40 -1.2 1,770,018 + BT1439 0.41 -1.7 1,770,082 + BT1439 0.45 -1.0 1,770,122 - BT1439 0.48 -0.8 1,770,192 - BT1439 0.52 +1.8 1,770,196 - BT1439 0.53 -1.1 1,770,196 - BT1439 0.53 +1.2 1,770,231 + BT1439 0.55 +2.7 1,770,280 - BT1439 0.58 +0.8 1,770,319 - BT1439 0.61 -1.0 1,770,319 - BT1439 0.61 +0.9
Or see this region's nucleotide sequence