Experiment: BHIS with Cephalothin 0.625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0099 and BT0100 are separated by 19 nucleotides BT0100 and BT0101 overlap by 16 nucleotides BT0101 and BT0102 are separated by 20 nucleotides
BT0099: BT0099 - hypothetical protein (NCBI ptt file), at 91,004 to 91,285
BT0099
BT0100: BT0100 - hypothetical protein (NCBI ptt file), at 91,305 to 91,742
BT0100
BT0101: BT0101 - conserved hypothetical protein (NCBI ptt file), at 91,727 to 92,965
BT0101
BT0102: BT0102 - conserved hypothetical protein (NCBI ptt file), at 92,986 to 94,995
BT0102
Position (kb) 91
92
93 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 91.597 kb on + strand, within BT0100 at 91.598 kb on - strand, within BT0100 at 91.610 kb on + strand, within BT0100 at 91.740 kb on + strand at 91.776 kb on - strand at 91.813 kb on - strand at 91.829 kb on - strand at 91.829 kb on - strand at 91.829 kb on - strand at 91.871 kb on - strand, within BT0101 at 91.931 kb on - strand, within BT0101 at 91.931 kb on - strand, within BT0101 at 91.931 kb on - strand, within BT0101 at 91.984 kb on + strand, within BT0101 at 92.006 kb on - strand, within BT0101 at 92.031 kb on - strand, within BT0101 at 92.031 kb on - strand, within BT0101 at 92.031 kb on - strand, within BT0101 at 92.071 kb on - strand, within BT0101 at 92.071 kb on - strand, within BT0101 at 92.074 kb on + strand, within BT0101 at 92.075 kb on - strand, within BT0101 at 92.263 kb on - strand, within BT0101 at 92.336 kb on - strand, within BT0101 at 92.336 kb on - strand, within BT0101 at 92.340 kb on - strand, within BT0101 at 92.347 kb on - strand, within BT0101 at 92.651 kb on - strand, within BT0101 at 92.662 kb on - strand, within BT0101 at 92.664 kb on - strand, within BT0101 at 92.664 kb on - strand, within BT0101 at 92.964 kb on - strand at 93.034 kb on - strand at 93.073 kb on + strand at 93.180 kb on - strand at 93.183 kb on + strand at 93.203 kb on + strand, within BT0102 at 93.204 kb on - strand, within BT0102 at 93.228 kb on + strand, within BT0102 at 93.271 kb on + strand, within BT0102 at 93.361 kb on + strand, within BT0102 at 93.362 kb on - strand, within BT0102 at 93.370 kb on - strand, within BT0102 at 93.507 kb on - strand, within BT0102 at 93.515 kb on + strand, within BT0102 at 93.541 kb on - strand, within BT0102 at 93.662 kb on + strand, within BT0102 at 93.682 kb on + strand, within BT0102 at 93.683 kb on - strand, within BT0102 at 93.688 kb on + strand, within BT0102 at 93.689 kb on - strand, within BT0102 at 93.689 kb on - strand, within BT0102 at 93.689 kb on - strand, within BT0102 at 93.724 kb on + strand, within BT0102 at 93.780 kb on - strand, within BT0102 at 93.802 kb on - strand, within BT0102 at 93.816 kb on + strand, within BT0102 at 93.871 kb on + strand, within BT0102 at 93.881 kb on - strand, within BT0102 at 93.884 kb on + strand, within BT0102
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.625 mg/ml remove 91,597 + BT0100 0.67 -2.1 91,598 - BT0100 0.67 -2.7 91,610 + BT0100 0.70 -1.0 91,740 + -1.1 91,776 - -0.9 91,813 - +0.3 91,829 - +1.4 91,829 - -2.1 91,829 - -1.8 91,871 - BT0101 0.12 +0.7 91,931 - BT0101 0.16 +0.7 91,931 - BT0101 0.16 -0.5 91,931 - BT0101 0.16 +1.1 91,984 + BT0101 0.21 -2.3 92,006 - BT0101 0.23 +0.2 92,031 - BT0101 0.25 -1.2 92,031 - BT0101 0.25 -0.0 92,031 - BT0101 0.25 -0.1 92,071 - BT0101 0.28 +0.3 92,071 - BT0101 0.28 +2.3 92,074 + BT0101 0.28 +0.2 92,075 - BT0101 0.28 +1.4 92,263 - BT0101 0.43 +0.1 92,336 - BT0101 0.49 +1.1 92,336 - BT0101 0.49 +0.7 92,340 - BT0101 0.49 -1.5 92,347 - BT0101 0.50 -2.3 92,651 - BT0101 0.75 +1.7 92,662 - BT0101 0.75 -1.4 92,664 - BT0101 0.76 -0.1 92,664 - BT0101 0.76 -1.6 92,964 - -0.8 93,034 - +2.7 93,073 + +1.0 93,180 - -0.1 93,183 + -2.3 93,203 + BT0102 0.11 +0.4 93,204 - BT0102 0.11 -1.4 93,228 + BT0102 0.12 +1.5 93,271 + BT0102 0.14 -1.8 93,361 + BT0102 0.19 +0.7 93,362 - BT0102 0.19 -1.7 93,370 - BT0102 0.19 -1.6 93,507 - BT0102 0.26 -0.5 93,515 + BT0102 0.26 -1.2 93,541 - BT0102 0.28 -0.3 93,662 + BT0102 0.34 +0.3 93,682 + BT0102 0.35 -2.0 93,683 - BT0102 0.35 -0.3 93,688 + BT0102 0.35 -1.6 93,689 - BT0102 0.35 +0.5 93,689 - BT0102 0.35 -1.8 93,689 - BT0102 0.35 +0.3 93,724 + BT0102 0.37 -1.3 93,780 - BT0102 0.40 -2.1 93,802 - BT0102 0.41 -0.4 93,816 + BT0102 0.41 -3.0 93,871 + BT0102 0.44 -0.9 93,881 - BT0102 0.45 +1.4 93,884 + BT0102 0.45 +1.3
Or see this region's nucleotide sequence