Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09005 and MMJJ_RS09010 are separated by 146 nucleotides MMJJ_RS09010 and MMJJ_RS09015 are separated by 77 nucleotides MMJJ_RS09015 and MMJJ_RS09020 are separated by 36 nucleotides
MMJJ_RS09005: MMJJ_RS09005 - N-6 DNA methylase, at 1,660,742 to 1,662,676
_RS09005
MMJJ_RS09010: MMJJ_RS09010 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,662,823 to 1,663,302
_RS09010
MMJJ_RS09015: MMJJ_RS09015 - SPFH domain-containing protein, at 1,663,380 to 1,664,186
_RS09015
MMJJ_RS09020: MMJJ_RS09020 - hypothetical protein, at 1,664,223 to 1,664,636
_RS09020
Position (kb)
1662
1663
1664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 1661.824 kb on + strand, within MMJJ_RS09005 at 1661.824 kb on + strand, within MMJJ_RS09005 at 1661.902 kb on - strand, within MMJJ_RS09005 at 1661.902 kb on - strand, within MMJJ_RS09005 at 1661.903 kb on + strand, within MMJJ_RS09005 at 1661.911 kb on - strand, within MMJJ_RS09005 at 1661.998 kb on - strand, within MMJJ_RS09005 at 1661.998 kb on - strand, within MMJJ_RS09005 at 1661.999 kb on + strand, within MMJJ_RS09005 at 1662.054 kb on + strand, within MMJJ_RS09005 at 1662.056 kb on - strand, within MMJJ_RS09005 at 1662.068 kb on - strand, within MMJJ_RS09005 at 1662.095 kb on + strand, within MMJJ_RS09005 at 1662.095 kb on + strand, within MMJJ_RS09005 at 1662.095 kb on + strand, within MMJJ_RS09005 at 1662.095 kb on + strand, within MMJJ_RS09005 at 1662.126 kb on + strand, within MMJJ_RS09005 at 1662.126 kb on + strand, within MMJJ_RS09005 at 1662.126 kb on + strand, within MMJJ_RS09005 at 1662.128 kb on - strand, within MMJJ_RS09005 at 1662.129 kb on + strand, within MMJJ_RS09005 at 1662.199 kb on - strand, within MMJJ_RS09005 at 1662.199 kb on - strand, within MMJJ_RS09005 at 1662.199 kb on - strand, within MMJJ_RS09005 at 1662.201 kb on - strand, within MMJJ_RS09005 at 1662.202 kb on + strand, within MMJJ_RS09005 at 1662.204 kb on - strand, within MMJJ_RS09005 at 1662.234 kb on + strand, within MMJJ_RS09005 at 1662.245 kb on - strand, within MMJJ_RS09005 at 1662.245 kb on - strand, within MMJJ_RS09005 at 1662.246 kb on + strand, within MMJJ_RS09005 at 1662.286 kb on + strand, within MMJJ_RS09005 at 1662.286 kb on + strand, within MMJJ_RS09005 at 1662.286 kb on + strand, within MMJJ_RS09005 at 1662.286 kb on + strand, within MMJJ_RS09005 at 1662.346 kb on - strand, within MMJJ_RS09005 at 1662.346 kb on - strand, within MMJJ_RS09005 at 1662.348 kb on - strand, within MMJJ_RS09005 at 1662.348 kb on - strand, within MMJJ_RS09005 at 1662.348 kb on - strand, within MMJJ_RS09005 at 1662.349 kb on + strand, within MMJJ_RS09005 at 1662.379 kb on + strand, within MMJJ_RS09005 at 1662.430 kb on - strand, within MMJJ_RS09005 at 1662.430 kb on - strand, within MMJJ_RS09005 at 1662.430 kb on - strand, within MMJJ_RS09005 at 1662.445 kb on - strand, within MMJJ_RS09005 at 1662.445 kb on - strand, within MMJJ_RS09005 at 1662.446 kb on + strand, within MMJJ_RS09005 at 1662.449 kb on - strand, within MMJJ_RS09005 at 1662.449 kb on - strand, within MMJJ_RS09005 at 1662.450 kb on + strand, within MMJJ_RS09005 at 1662.455 kb on - strand, within MMJJ_RS09005 at 1662.456 kb on + strand, within MMJJ_RS09005 at 1662.456 kb on + strand, within MMJJ_RS09005 at 1662.456 kb on + strand, within MMJJ_RS09005 at 1662.456 kb on + strand, within MMJJ_RS09005 at 1662.456 kb on + strand, within MMJJ_RS09005 at 1662.457 kb on - strand, within MMJJ_RS09005 at 1662.457 kb on - strand, within MMJJ_RS09005 at 1662.457 kb on - strand, within MMJJ_RS09005 at 1662.457 kb on - strand, within MMJJ_RS09005 at 1662.458 kb on + strand, within MMJJ_RS09005 at 1662.458 kb on + strand, within MMJJ_RS09005 at 1662.458 kb on + strand, within MMJJ_RS09005 at 1662.463 kb on - strand, within MMJJ_RS09005 at 1662.464 kb on + strand, within MMJJ_RS09005 at 1662.509 kb on - strand at 1662.510 kb on + strand at 1662.510 kb on + strand at 1662.510 kb on + strand at 1662.520 kb on + strand at 1662.520 kb on + strand at 1662.520 kb on + strand at 1662.697 kb on + strand at 1662.699 kb on - strand at 1662.699 kb on - strand at 1662.700 kb on + strand at 1662.727 kb on + strand at 1662.728 kb on - strand at 1662.728 kb on - strand at 1662.728 kb on - strand at 1662.787 kb on + strand at 1662.828 kb on - strand at 1662.828 kb on - strand at 1662.828 kb on - strand at 1662.829 kb on + strand at 1662.829 kb on + strand at 1662.834 kb on + strand at 1662.849 kb on - strand at 1662.850 kb on + strand at 1662.895 kb on - strand, within MMJJ_RS09010 at 1662.895 kb on - strand, within MMJJ_RS09010 at 1662.895 kb on - strand, within MMJJ_RS09010 at 1662.933 kb on - strand, within MMJJ_RS09010 at 1662.994 kb on - strand, within MMJJ_RS09010 at 1662.995 kb on + strand, within MMJJ_RS09010 at 1663.055 kb on + strand, within MMJJ_RS09010 at 1663.055 kb on + strand, within MMJJ_RS09010 at 1663.139 kb on + strand, within MMJJ_RS09010 at 1663.235 kb on - strand, within MMJJ_RS09010 at 1663.242 kb on - strand, within MMJJ_RS09010 at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.255 kb on - strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.256 kb on + strand at 1663.259 kb on - strand at 1663.259 kb on - strand at 1663.271 kb on + strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.294 kb on - strand at 1663.302 kb on - strand at 1663.304 kb on - strand at 1663.329 kb on + strand at 1663.378 kb on + strand at 1663.464 kb on - strand, within MMJJ_RS09015 at 1663.683 kb on + strand, within MMJJ_RS09015 at 1663.767 kb on - strand, within MMJJ_RS09015 at 1663.819 kb on + strand, within MMJJ_RS09015 at 1663.830 kb on - strand, within MMJJ_RS09015 at 1663.860 kb on - strand, within MMJJ_RS09015 at 1663.862 kb on - strand, within MMJJ_RS09015 at 1663.863 kb on + strand, within MMJJ_RS09015 at 1663.906 kb on + strand, within MMJJ_RS09015 at 1663.925 kb on + strand, within MMJJ_RS09015 at 1663.964 kb on - strand, within MMJJ_RS09015 at 1663.993 kb on + strand, within MMJJ_RS09015 at 1663.998 kb on + strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.004 kb on - strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.005 kb on + strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.010 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.016 kb on - strand, within MMJJ_RS09015 at 1664.022 kb on - strand, within MMJJ_RS09015 at 1664.023 kb on + strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.040 kb on - strand, within MMJJ_RS09015 at 1664.049 kb on + strand, within MMJJ_RS09015 at 1664.091 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.093 kb on - strand, within MMJJ_RS09015 at 1664.094 kb on + strand, within MMJJ_RS09015 at 1664.133 kb on - strand at 1664.134 kb on + strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.136 kb on - strand at 1664.137 kb on + strand at 1664.139 kb on - strand at 1664.142 kb on - strand at 1664.143 kb on + strand at 1664.155 kb on + strand at 1664.156 kb on - strand at 1664.157 kb on + strand at 1664.171 kb on - strand at 1664.186 kb on - strand at 1664.186 kb on - strand at 1664.187 kb on + strand at 1664.187 kb on + strand at 1664.198 kb on - strand at 1664.211 kb on - strand at 1664.218 kb on - strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.219 kb on + strand at 1664.220 kb on - strand at 1664.221 kb on + strand at 1664.232 kb on - strand at 1664.232 kb on - strand at 1664.248 kb on + strand at 1664.254 kb on + strand at 1664.254 kb on + strand at 1664.265 kb on + strand, within MMJJ_RS09020 at 1664.268 kb on - strand, within MMJJ_RS09020 at 1664.269 kb on + strand, within MMJJ_RS09020
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 1,661,824 + MMJJ_RS09005 0.56 +0.2 1,661,824 + MMJJ_RS09005 0.56 +0.7 1,661,902 - MMJJ_RS09005 0.60 +0.9 1,661,902 - MMJJ_RS09005 0.60 +0.5 1,661,903 + MMJJ_RS09005 0.60 -0.7 1,661,911 - MMJJ_RS09005 0.60 -0.1 1,661,998 - MMJJ_RS09005 0.65 -0.7 1,661,998 - MMJJ_RS09005 0.65 +0.9 1,661,999 + MMJJ_RS09005 0.65 +1.5 1,662,054 + MMJJ_RS09005 0.68 -0.3 1,662,056 - MMJJ_RS09005 0.68 -0.1 1,662,068 - MMJJ_RS09005 0.69 -0.9 1,662,095 + MMJJ_RS09005 0.70 +0.7 1,662,095 + MMJJ_RS09005 0.70 +0.5 1,662,095 + MMJJ_RS09005 0.70 -0.6 1,662,095 + MMJJ_RS09005 0.70 -3.0 1,662,126 + MMJJ_RS09005 0.72 +0.1 1,662,126 + MMJJ_RS09005 0.72 -2.2 1,662,126 + MMJJ_RS09005 0.72 +0.2 1,662,128 - MMJJ_RS09005 0.72 +1.6 1,662,129 + MMJJ_RS09005 0.72 +0.1 1,662,199 - MMJJ_RS09005 0.75 +0.5 1,662,199 - MMJJ_RS09005 0.75 +1.9 1,662,199 - MMJJ_RS09005 0.75 +0.4 1,662,201 - MMJJ_RS09005 0.75 +1.2 1,662,202 + MMJJ_RS09005 0.75 -0.5 1,662,204 - MMJJ_RS09005 0.76 -0.3 1,662,234 + MMJJ_RS09005 0.77 -1.6 1,662,245 - MMJJ_RS09005 0.78 -1.8 1,662,245 - MMJJ_RS09005 0.78 -0.1 1,662,246 + MMJJ_RS09005 0.78 +2.1 1,662,286 + MMJJ_RS09005 0.80 +0.6 1,662,286 + MMJJ_RS09005 0.80 -1.5 1,662,286 + MMJJ_RS09005 0.80 +0.8 1,662,286 + MMJJ_RS09005 0.80 +0.4 1,662,346 - MMJJ_RS09005 0.83 -1.1 1,662,346 - MMJJ_RS09005 0.83 +1.4 1,662,348 - MMJJ_RS09005 0.83 -1.5 1,662,348 - MMJJ_RS09005 0.83 +0.8 1,662,348 - MMJJ_RS09005 0.83 +0.2 1,662,349 + MMJJ_RS09005 0.83 +1.7 1,662,379 + MMJJ_RS09005 0.85 +1.6 1,662,430 - MMJJ_RS09005 0.87 -0.3 1,662,430 - MMJJ_RS09005 0.87 -1.9 1,662,430 - MMJJ_RS09005 0.87 +5.8 1,662,445 - MMJJ_RS09005 0.88 +3.9 1,662,445 - MMJJ_RS09005 0.88 -1.0 1,662,446 + MMJJ_RS09005 0.88 -0.0 1,662,449 - MMJJ_RS09005 0.88 -0.9 1,662,449 - MMJJ_RS09005 0.88 +0.1 1,662,450 + MMJJ_RS09005 0.88 -0.2 1,662,455 - MMJJ_RS09005 0.89 +0.4 1,662,456 + MMJJ_RS09005 0.89 -0.5 1,662,456 + MMJJ_RS09005 0.89 +0.2 1,662,456 + MMJJ_RS09005 0.89 +0.5 1,662,456 + MMJJ_RS09005 0.89 -0.5 1,662,456 + MMJJ_RS09005 0.89 -1.6 1,662,457 - MMJJ_RS09005 0.89 +0.6 1,662,457 - MMJJ_RS09005 0.89 +1.4 1,662,457 - MMJJ_RS09005 0.89 +0.1 1,662,457 - MMJJ_RS09005 0.89 +1.4 1,662,458 + MMJJ_RS09005 0.89 +0.3 1,662,458 + MMJJ_RS09005 0.89 -1.0 1,662,458 + MMJJ_RS09005 0.89 +0.6 1,662,463 - MMJJ_RS09005 0.89 -1.3 1,662,464 + MMJJ_RS09005 0.89 +0.8 1,662,509 - +0.3 1,662,510 + -0.3 1,662,510 + +0.0 1,662,510 + +0.4 1,662,520 + +0.4 1,662,520 + +1.8 1,662,520 + +0.7 1,662,697 + +0.4 1,662,699 - +1.9 1,662,699 - +1.6 1,662,700 + +2.3 1,662,727 + -1.4 1,662,728 - +1.8 1,662,728 - -0.7 1,662,728 - -0.3 1,662,787 + +0.6 1,662,828 - +9.0 1,662,828 - +0.4 1,662,828 - -0.4 1,662,829 + -0.4 1,662,829 + +0.5 1,662,834 + -1.9 1,662,849 - +0.6 1,662,850 + -1.7 1,662,895 - MMJJ_RS09010 0.15 +0.4 1,662,895 - MMJJ_RS09010 0.15 -1.9 1,662,895 - MMJJ_RS09010 0.15 -0.9 1,662,933 - MMJJ_RS09010 0.23 -1.9 1,662,994 - MMJJ_RS09010 0.36 -1.0 1,662,995 + MMJJ_RS09010 0.36 -1.4 1,663,055 + MMJJ_RS09010 0.48 +0.8 1,663,055 + MMJJ_RS09010 0.48 -1.9 1,663,139 + MMJJ_RS09010 0.66 -0.3 1,663,235 - MMJJ_RS09010 0.86 -0.9 1,663,242 - MMJJ_RS09010 0.87 -1.4 1,663,255 - +1.4 1,663,255 - -0.5 1,663,255 - +0.5 1,663,255 - +0.6 1,663,255 - -0.3 1,663,255 - -0.0 1,663,256 + +0.4 1,663,256 + +1.2 1,663,256 + +0.3 1,663,259 - -0.7 1,663,259 - -0.4 1,663,271 + -1.9 1,663,294 - +0.8 1,663,294 - +0.8 1,663,294 - +0.2 1,663,302 - -1.1 1,663,304 - -0.0 1,663,329 + +2.3 1,663,378 + +1.2 1,663,464 - MMJJ_RS09015 0.10 -0.7 1,663,683 + MMJJ_RS09015 0.38 -1.7 1,663,767 - MMJJ_RS09015 0.48 +1.8 1,663,819 + MMJJ_RS09015 0.54 -0.2 1,663,830 - MMJJ_RS09015 0.56 -2.7 1,663,860 - MMJJ_RS09015 0.59 -0.5 1,663,862 - MMJJ_RS09015 0.60 -0.6 1,663,863 + MMJJ_RS09015 0.60 +1.9 1,663,906 + MMJJ_RS09015 0.65 +0.4 1,663,925 + MMJJ_RS09015 0.68 -1.2 1,663,964 - MMJJ_RS09015 0.72 +0.4 1,663,993 + MMJJ_RS09015 0.76 -0.7 1,663,998 + MMJJ_RS09015 0.77 +1.2 1,664,004 - MMJJ_RS09015 0.77 +0.4 1,664,004 - MMJJ_RS09015 0.77 -0.3 1,664,004 - MMJJ_RS09015 0.77 -0.5 1,664,004 - MMJJ_RS09015 0.77 -0.4 1,664,004 - MMJJ_RS09015 0.77 +0.6 1,664,004 - MMJJ_RS09015 0.77 -1.0 1,664,005 + MMJJ_RS09015 0.77 +2.3 1,664,005 + MMJJ_RS09015 0.77 +1.1 1,664,005 + MMJJ_RS09015 0.77 +0.5 1,664,010 - MMJJ_RS09015 0.78 +1.1 1,664,010 - MMJJ_RS09015 0.78 +0.6 1,664,010 - MMJJ_RS09015 0.78 -0.2 1,664,016 - MMJJ_RS09015 0.79 -2.1 1,664,016 - MMJJ_RS09015 0.79 -0.7 1,664,016 - MMJJ_RS09015 0.79 -0.4 1,664,022 - MMJJ_RS09015 0.80 +0.6 1,664,023 + MMJJ_RS09015 0.80 +0.4 1,664,040 - MMJJ_RS09015 0.82 -0.4 1,664,040 - MMJJ_RS09015 0.82 -2.1 1,664,049 + MMJJ_RS09015 0.83 +0.0 1,664,091 - MMJJ_RS09015 0.88 +1.4 1,664,093 - MMJJ_RS09015 0.88 +0.4 1,664,093 - MMJJ_RS09015 0.88 +0.0 1,664,094 + MMJJ_RS09015 0.88 -0.6 1,664,133 - -1.5 1,664,134 + -0.7 1,664,136 - -0.8 1,664,136 - -2.3 1,664,136 - +0.3 1,664,137 + -0.3 1,664,139 - -1.1 1,664,142 - -0.7 1,664,143 + +1.2 1,664,155 + +2.1 1,664,156 - -1.3 1,664,157 + -0.4 1,664,171 - -1.9 1,664,186 - +1.1 1,664,186 - +1.2 1,664,187 + +1.3 1,664,187 + -0.3 1,664,198 - +0.3 1,664,211 - -0.6 1,664,218 - -0.9 1,664,219 + -0.5 1,664,219 + -0.7 1,664,219 + +0.2 1,664,220 - -3.1 1,664,221 + -0.4 1,664,232 - -3.0 1,664,232 - -0.2 1,664,248 + +0.8 1,664,254 + -0.1 1,664,254 + -1.0 1,664,265 + MMJJ_RS09020 0.10 +0.1 1,664,268 - MMJJ_RS09020 0.11 -0.1 1,664,269 + MMJJ_RS09020 0.11 +0.7
Or see this region's nucleotide sequence