Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08170 and MMJJ_RS08175 are separated by 136 nucleotides MMJJ_RS08175 and MMJJ_RS08180 are separated by 103 nucleotides MMJJ_RS08180 and MMJJ_RS08185 are separated by 42 nucleotides
MMJJ_RS08170: MMJJ_RS08170 - diaminopimelate decarboxylase, at 1,500,165 to 1,501,475
_RS08170
MMJJ_RS08175: MMJJ_RS08175 - winged helix-turn-helix transcriptional regulator, at 1,501,612 to 1,502,466
_RS08175
MMJJ_RS08180: MMJJ_RS08180 - ABC transporter ATP-binding protein, at 1,502,570 to 1,503,334
_RS08180
MMJJ_RS08185: MMJJ_RS08185 - ABC transporter permease, at 1,503,377 to 1,504,138
_RS08185
Position (kb)
1501
1502
1503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 1500.768 kb on - strand, within MMJJ_RS08170 at 1501.487 kb on + strand at 1501.522 kb on + strand at 1501.526 kb on + strand at 1501.538 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.544 kb on + strand at 1501.544 kb on + strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.548 kb on + strand at 1501.548 kb on + strand at 1501.548 kb on + strand at 1501.560 kb on + strand at 1501.563 kb on - strand at 1501.564 kb on + strand at 1501.567 kb on + strand at 1501.603 kb on + strand at 1501.609 kb on - strand at 1501.613 kb on - strand at 1501.631 kb on - strand at 1501.631 kb on - strand at 1501.631 kb on - strand at 1501.632 kb on + strand at 1501.691 kb on + strand at 1501.704 kb on - strand, within MMJJ_RS08175 at 1501.761 kb on - strand, within MMJJ_RS08175 at 1501.762 kb on + strand, within MMJJ_RS08175 at 1501.838 kb on + strand, within MMJJ_RS08175 at 1501.879 kb on + strand, within MMJJ_RS08175 at 1501.910 kb on + strand, within MMJJ_RS08175 at 1501.966 kb on - strand, within MMJJ_RS08175 at 1502.014 kb on + strand, within MMJJ_RS08175 at 1502.061 kb on + strand, within MMJJ_RS08175 at 1502.061 kb on + strand, within MMJJ_RS08175 at 1502.084 kb on + strand, within MMJJ_RS08175 at 1502.084 kb on + strand, within MMJJ_RS08175 at 1502.092 kb on - strand, within MMJJ_RS08175 at 1502.093 kb on + strand, within MMJJ_RS08175 at 1502.121 kb on + strand, within MMJJ_RS08175 at 1502.131 kb on - strand, within MMJJ_RS08175 at 1502.134 kb on + strand, within MMJJ_RS08175 at 1502.142 kb on - strand, within MMJJ_RS08175 at 1502.148 kb on - strand, within MMJJ_RS08175 at 1502.185 kb on + strand, within MMJJ_RS08175 at 1502.188 kb on - strand, within MMJJ_RS08175 at 1502.190 kb on - strand, within MMJJ_RS08175 at 1502.199 kb on - strand, within MMJJ_RS08175 at 1502.205 kb on - strand, within MMJJ_RS08175 at 1502.205 kb on - strand, within MMJJ_RS08175 at 1502.265 kb on + strand, within MMJJ_RS08175 at 1502.374 kb on - strand, within MMJJ_RS08175 at 1502.421 kb on - strand at 1502.422 kb on + strand at 1502.422 kb on + strand at 1502.424 kb on - strand at 1502.425 kb on + strand at 1502.428 kb on - strand at 1502.428 kb on - strand at 1502.466 kb on - strand at 1502.466 kb on - strand at 1502.467 kb on + strand at 1502.467 kb on + strand at 1502.509 kb on - strand at 1502.509 kb on - strand at 1502.551 kb on + strand at 1502.551 kb on + strand at 1502.558 kb on + strand at 1502.560 kb on - strand at 1502.560 kb on - strand at 1502.560 kb on - strand at 1502.561 kb on + strand at 1502.561 kb on + strand at 1502.561 kb on + strand at 1502.572 kb on + strand at 1502.577 kb on - strand at 1502.577 kb on - strand at 1502.578 kb on + strand at 1502.626 kb on + strand at 1502.672 kb on - strand, within MMJJ_RS08180 at 1502.672 kb on - strand, within MMJJ_RS08180 at 1502.672 kb on - strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.673 kb on + strand, within MMJJ_RS08180 at 1502.691 kb on + strand, within MMJJ_RS08180 at 1502.736 kb on - strand, within MMJJ_RS08180 at 1502.737 kb on + strand, within MMJJ_RS08180 at 1502.762 kb on - strand, within MMJJ_RS08180 at 1502.763 kb on + strand, within MMJJ_RS08180 at 1502.817 kb on + strand, within MMJJ_RS08180 at 1502.865 kb on + strand, within MMJJ_RS08180 at 1502.931 kb on + strand, within MMJJ_RS08180 at 1502.931 kb on + strand, within MMJJ_RS08180 at 1502.943 kb on - strand, within MMJJ_RS08180 at 1502.981 kb on + strand, within MMJJ_RS08180 at 1503.092 kb on - strand, within MMJJ_RS08180 at 1503.141 kb on - strand, within MMJJ_RS08180 at 1503.335 kb on + strand at 1503.335 kb on + strand at 1503.361 kb on + strand at 1503.362 kb on - strand at 1503.378 kb on - strand at 1503.419 kb on + strand at 1503.420 kb on - strand at 1503.420 kb on - strand at 1503.420 kb on - strand at 1503.452 kb on - strand at 1503.452 kb on - strand at 1503.453 kb on + strand at 1503.453 kb on + strand at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.460 kb on - strand, within MMJJ_RS08185 at 1503.461 kb on + strand, within MMJJ_RS08185 at 1503.461 kb on + strand, within MMJJ_RS08185 at 1503.464 kb on - strand, within MMJJ_RS08185
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 1,500,768 - MMJJ_RS08170 0.46 +0.6 1,501,487 + +1.6 1,501,522 + +0.7 1,501,526 + +0.9 1,501,538 - +1.6 1,501,543 - +1.9 1,501,543 - -1.5 1,501,543 - +1.6 1,501,543 - +1.7 1,501,544 + -0.9 1,501,544 + +2.9 1,501,545 - +0.7 1,501,545 - +1.4 1,501,545 - -0.9 1,501,545 - +2.3 1,501,545 - +0.6 1,501,545 - -1.5 1,501,545 - -0.0 1,501,545 - -2.3 1,501,545 - +0.1 1,501,545 - +2.3 1,501,546 + -0.2 1,501,546 + +0.8 1,501,546 + +0.9 1,501,546 + +0.4 1,501,546 + +1.1 1,501,546 + +1.6 1,501,547 - -0.1 1,501,547 - -0.5 1,501,547 - -0.8 1,501,547 - -1.4 1,501,548 + -1.4 1,501,548 + -0.1 1,501,548 + +1.1 1,501,560 + +0.6 1,501,563 - -0.8 1,501,564 + -1.1 1,501,567 + -1.0 1,501,603 + -0.4 1,501,609 - -0.3 1,501,613 - -2.5 1,501,631 - +1.5 1,501,631 - +0.3 1,501,631 - -0.4 1,501,632 + -1.8 1,501,691 + +0.6 1,501,704 - MMJJ_RS08175 0.11 +1.0 1,501,761 - MMJJ_RS08175 0.17 -1.5 1,501,762 + MMJJ_RS08175 0.18 -1.7 1,501,838 + MMJJ_RS08175 0.26 -1.8 1,501,879 + MMJJ_RS08175 0.31 -1.7 1,501,910 + MMJJ_RS08175 0.35 -1.2 1,501,966 - MMJJ_RS08175 0.41 -0.7 1,502,014 + MMJJ_RS08175 0.47 -2.6 1,502,061 + MMJJ_RS08175 0.53 +0.3 1,502,061 + MMJJ_RS08175 0.53 -1.6 1,502,084 + MMJJ_RS08175 0.55 -0.6 1,502,084 + MMJJ_RS08175 0.55 -1.0 1,502,092 - MMJJ_RS08175 0.56 +0.9 1,502,093 + MMJJ_RS08175 0.56 -0.8 1,502,121 + MMJJ_RS08175 0.60 -2.0 1,502,131 - MMJJ_RS08175 0.61 -0.4 1,502,134 + MMJJ_RS08175 0.61 -0.3 1,502,142 - MMJJ_RS08175 0.62 -1.3 1,502,148 - MMJJ_RS08175 0.63 +1.2 1,502,185 + MMJJ_RS08175 0.67 -0.3 1,502,188 - MMJJ_RS08175 0.67 -1.0 1,502,190 - MMJJ_RS08175 0.68 +0.8 1,502,199 - MMJJ_RS08175 0.69 +0.6 1,502,205 - MMJJ_RS08175 0.69 -2.3 1,502,205 - MMJJ_RS08175 0.69 +0.0 1,502,265 + MMJJ_RS08175 0.76 -1.3 1,502,374 - MMJJ_RS08175 0.89 -0.1 1,502,421 - -1.8 1,502,422 + -0.9 1,502,422 + +0.7 1,502,424 - -1.9 1,502,425 + -0.7 1,502,428 - -1.6 1,502,428 - +0.9 1,502,466 - -1.0 1,502,466 - -0.6 1,502,467 + -0.0 1,502,467 + -1.7 1,502,509 - +0.5 1,502,509 - -1.5 1,502,551 + -0.2 1,502,551 + +2.0 1,502,558 + -1.0 1,502,560 - -0.7 1,502,560 - +0.6 1,502,560 - -0.0 1,502,561 + -0.2 1,502,561 + +0.4 1,502,561 + -1.1 1,502,572 + -1.9 1,502,577 - -0.0 1,502,577 - +0.5 1,502,578 + -1.9 1,502,626 + +0.9 1,502,672 - MMJJ_RS08180 0.13 -0.9 1,502,672 - MMJJ_RS08180 0.13 -1.3 1,502,672 - MMJJ_RS08180 0.13 -1.4 1,502,673 + MMJJ_RS08180 0.13 -1.2 1,502,673 + MMJJ_RS08180 0.13 -1.5 1,502,673 + MMJJ_RS08180 0.13 +1.6 1,502,673 + MMJJ_RS08180 0.13 -1.1 1,502,673 + MMJJ_RS08180 0.13 -2.4 1,502,673 + MMJJ_RS08180 0.13 -2.0 1,502,673 + MMJJ_RS08180 0.13 +0.0 1,502,691 + MMJJ_RS08180 0.16 -0.9 1,502,736 - MMJJ_RS08180 0.22 -1.5 1,502,737 + MMJJ_RS08180 0.22 -1.1 1,502,762 - MMJJ_RS08180 0.25 -2.0 1,502,763 + MMJJ_RS08180 0.25 -1.1 1,502,817 + MMJJ_RS08180 0.32 +7.4 1,502,865 + MMJJ_RS08180 0.39 -0.6 1,502,931 + MMJJ_RS08180 0.47 -1.1 1,502,931 + MMJJ_RS08180 0.47 -1.2 1,502,943 - MMJJ_RS08180 0.49 -1.6 1,502,981 + MMJJ_RS08180 0.54 -0.9 1,503,092 - MMJJ_RS08180 0.68 -3.0 1,503,141 - MMJJ_RS08180 0.75 +0.3 1,503,335 + -0.7 1,503,335 + -1.4 1,503,361 + -0.7 1,503,362 - -0.6 1,503,378 - -1.9 1,503,419 + -3.4 1,503,420 - -0.0 1,503,420 - -0.7 1,503,420 - -2.2 1,503,452 - -3.1 1,503,452 - -1.1 1,503,453 + -0.9 1,503,453 + -0.7 1,503,460 - MMJJ_RS08185 0.11 +0.3 1,503,460 - MMJJ_RS08185 0.11 -0.7 1,503,460 - MMJJ_RS08185 0.11 -2.7 1,503,461 + MMJJ_RS08185 0.11 -1.9 1,503,461 + MMJJ_RS08185 0.11 -1.5 1,503,464 - MMJJ_RS08185 0.11 -1.7
Or see this region's nucleotide sequence