Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04140 and MMJJ_RS04145 are separated by 47 nucleotides MMJJ_RS04145 and MMJJ_RS04150 are separated by 9 nucleotides MMJJ_RS04150 and MMJJ_RS04155 are separated by 9 nucleotides MMJJ_RS04155 and MMJJ_RS04160 are separated by 78 nucleotides
MMJJ_RS04140: MMJJ_RS04140 - iron-containing alcohol dehydrogenase, at 769,344 to 770,348
_RS04140
MMJJ_RS04145: MMJJ_RS04145 - glutamate-1-semialdehyde 2,1-aminomutase, at 770,396 to 771,679
_RS04145
MMJJ_RS04150: MMJJ_RS04150 - DUF5379 domain-containing protein, at 771,689 to 771,967
_RS04150
MMJJ_RS04155: MMJJ_RS04155 - hypothetical protein, at 771,977 to 772,216
_RS04155
MMJJ_RS04160: MMJJ_RS04160 - sodium/proline symporter PutP, at 772,295 to 773,773
_RS04160
Position (kb) 770
771
772 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 770.463 kb on + strand at 770.470 kb on - strand at 770.498 kb on - strand at 770.505 kb on + strand at 770.526 kb on - strand, within MMJJ_RS04145 at 770.533 kb on + strand, within MMJJ_RS04145 at 770.533 kb on + strand, within MMJJ_RS04145 at 770.533 kb on + strand, within MMJJ_RS04145 at 770.575 kb on + strand, within MMJJ_RS04145 at 770.621 kb on - strand, within MMJJ_RS04145 at 770.621 kb on - strand, within MMJJ_RS04145 at 770.622 kb on + strand, within MMJJ_RS04145 at 770.622 kb on + strand, within MMJJ_RS04145 at 770.698 kb on - strand, within MMJJ_RS04145 at 770.794 kb on + strand, within MMJJ_RS04145 at 770.843 kb on - strand, within MMJJ_RS04145 at 770.913 kb on - strand, within MMJJ_RS04145 at 771.071 kb on + strand, within MMJJ_RS04145 at 771.154 kb on - strand, within MMJJ_RS04145 at 771.224 kb on - strand, within MMJJ_RS04145 at 771.225 kb on + strand, within MMJJ_RS04145 at 771.225 kb on + strand, within MMJJ_RS04145 at 771.271 kb on - strand, within MMJJ_RS04145 at 771.272 kb on + strand, within MMJJ_RS04145 at 771.283 kb on - strand, within MMJJ_RS04145 at 771.303 kb on + strand, within MMJJ_RS04145 at 771.367 kb on - strand, within MMJJ_RS04145 at 771.367 kb on - strand, within MMJJ_RS04145 at 771.456 kb on + strand, within MMJJ_RS04145 at 771.458 kb on - strand, within MMJJ_RS04145 at 771.460 kb on - strand, within MMJJ_RS04145 at 771.500 kb on - strand, within MMJJ_RS04145 at 771.642 kb on + strand at 771.709 kb on + strand at 771.709 kb on + strand at 771.726 kb on + strand, within MMJJ_RS04150 at 771.752 kb on - strand, within MMJJ_RS04150 at 771.801 kb on + strand, within MMJJ_RS04150 at 771.827 kb on + strand, within MMJJ_RS04150 at 771.878 kb on - strand, within MMJJ_RS04150 at 771.897 kb on - strand at 771.973 kb on + strand at 771.973 kb on + strand at 771.979 kb on + strand at 772.043 kb on + strand, within MMJJ_RS04155 at 772.044 kb on - strand, within MMJJ_RS04155 at 772.044 kb on - strand, within MMJJ_RS04155 at 772.048 kb on - strand at 772.048 kb on - strand, within MMJJ_RS04155 at 772.048 kb on - strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.049 kb on + strand, within MMJJ_RS04155 at 772.055 kb on - strand, within MMJJ_RS04155 at 772.061 kb on - strand at 772.067 kb on - strand, within MMJJ_RS04155 at 772.068 kb on + strand, within MMJJ_RS04155 at 772.068 kb on + strand at 772.068 kb on + strand, within MMJJ_RS04155 at 772.068 kb on + strand, within MMJJ_RS04155 at 772.079 kb on - strand, within MMJJ_RS04155 at 772.084 kb on + strand, within MMJJ_RS04155 at 772.099 kb on - strand, within MMJJ_RS04155 at 772.104 kb on + strand at 772.117 kb on - strand, within MMJJ_RS04155 at 772.117 kb on - strand, within MMJJ_RS04155 at 772.121 kb on - strand, within MMJJ_RS04155 at 772.173 kb on + strand, within MMJJ_RS04155 at 772.216 kb on - strand at 772.230 kb on + strand at 772.247 kb on - strand at 772.247 kb on - strand at 772.256 kb on - strand at 772.257 kb on + strand at 772.260 kb on - strand at 772.260 kb on - strand at 772.260 kb on - strand at 772.261 kb on + strand at 772.269 kb on - strand at 772.269 kb on - strand at 772.270 kb on + strand at 772.276 kb on - strand at 772.287 kb on - strand at 772.340 kb on - strand at 772.367 kb on - strand at 772.457 kb on - strand, within MMJJ_RS04160 at 772.617 kb on - strand, within MMJJ_RS04160 at 772.620 kb on - strand, within MMJJ_RS04160 at 772.621 kb on + strand, within MMJJ_RS04160 at 772.625 kb on - strand, within MMJJ_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 770,463 + +1.0 770,470 - -0.5 770,498 - -2.0 770,505 + -1.6 770,526 - MMJJ_RS04145 0.10 +0.5 770,533 + MMJJ_RS04145 0.11 -0.3 770,533 + MMJJ_RS04145 0.11 -1.8 770,533 + MMJJ_RS04145 0.11 +1.8 770,575 + MMJJ_RS04145 0.14 +0.2 770,621 - MMJJ_RS04145 0.18 -1.5 770,621 - MMJJ_RS04145 0.18 -0.2 770,622 + MMJJ_RS04145 0.18 -1.8 770,622 + MMJJ_RS04145 0.18 -0.1 770,698 - MMJJ_RS04145 0.24 -1.3 770,794 + MMJJ_RS04145 0.31 +0.8 770,843 - MMJJ_RS04145 0.35 -0.0 770,913 - MMJJ_RS04145 0.40 -0.1 771,071 + MMJJ_RS04145 0.53 +0.0 771,154 - MMJJ_RS04145 0.59 -0.4 771,224 - MMJJ_RS04145 0.64 +1.0 771,225 + MMJJ_RS04145 0.65 -0.6 771,225 + MMJJ_RS04145 0.65 -0.6 771,271 - MMJJ_RS04145 0.68 -1.3 771,272 + MMJJ_RS04145 0.68 -0.1 771,283 - MMJJ_RS04145 0.69 -0.5 771,303 + MMJJ_RS04145 0.71 -0.3 771,367 - MMJJ_RS04145 0.76 -0.5 771,367 - MMJJ_RS04145 0.76 -0.1 771,456 + MMJJ_RS04145 0.83 -0.4 771,458 - MMJJ_RS04145 0.83 -0.9 771,460 - MMJJ_RS04145 0.83 -0.4 771,500 - MMJJ_RS04145 0.86 -1.3 771,642 + +0.8 771,709 + +1.1 771,709 + -0.7 771,726 + MMJJ_RS04150 0.13 +0.6 771,752 - MMJJ_RS04150 0.23 +2.1 771,801 + MMJJ_RS04150 0.40 +0.2 771,827 + MMJJ_RS04150 0.49 +1.5 771,878 - MMJJ_RS04150 0.68 -1.5 771,897 - +1.3 771,973 + +0.5 771,973 + -0.6 771,979 + -0.4 772,043 + MMJJ_RS04155 0.28 -1.2 772,044 - MMJJ_RS04155 0.28 -1.4 772,044 - MMJJ_RS04155 0.28 +1.6 772,048 - +3.6 772,048 - MMJJ_RS04155 0.30 +0.5 772,048 - MMJJ_RS04155 0.30 +0.5 772,049 + MMJJ_RS04155 0.30 -0.7 772,049 + MMJJ_RS04155 0.30 -0.4 772,049 + MMJJ_RS04155 0.30 -1.1 772,049 + MMJJ_RS04155 0.30 +6.2 772,049 + MMJJ_RS04155 0.30 +0.5 772,049 + MMJJ_RS04155 0.30 +0.5 772,055 - MMJJ_RS04155 0.33 -0.3 772,061 - +2.1 772,067 - MMJJ_RS04155 0.38 -0.7 772,068 + MMJJ_RS04155 0.38 +0.2 772,068 + +0.8 772,068 + MMJJ_RS04155 0.38 +0.6 772,068 + MMJJ_RS04155 0.38 -1.2 772,079 - MMJJ_RS04155 0.42 -0.4 772,084 + MMJJ_RS04155 0.45 +0.3 772,099 - MMJJ_RS04155 0.51 +0.4 772,104 + +2.6 772,117 - MMJJ_RS04155 0.58 +0.3 772,117 - MMJJ_RS04155 0.58 -0.7 772,121 - MMJJ_RS04155 0.60 -0.1 772,173 + MMJJ_RS04155 0.82 -0.9 772,216 - -1.5 772,230 + +0.6 772,247 - +0.2 772,247 - +1.3 772,256 - -0.1 772,257 + +1.1 772,260 - +0.2 772,260 - +0.2 772,260 - +1.1 772,261 + -1.3 772,269 - +0.6 772,269 - +0.6 772,270 + -0.5 772,276 - -0.9 772,287 - -1.2 772,340 - +0.7 772,367 - -0.3 772,457 - MMJJ_RS04160 0.11 -0.8 772,617 - MMJJ_RS04160 0.22 -2.8 772,620 - MMJJ_RS04160 0.22 -0.3 772,621 + MMJJ_RS04160 0.22 +1.7 772,625 - MMJJ_RS04160 0.22 -0.0
Or see this region's nucleotide sequence