Experiment: Recovery broth with lactate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13905 and BBR_RS13910 overlap by 4 nucleotides BBR_RS13910 and BBR_RS13915 are separated by 155 nucleotides BBR_RS13915 and BBR_RS20805 are separated by 366 nucleotides
BBR_RS13905: BBR_RS13905 - ornithine carbamoyltransferase, at 899,670 to 900,635
_RS13905
BBR_RS13910: BBR_RS13910 - arginine repressor, at 900,632 to 901,144
_RS13910
BBR_RS13915: BBR_RS13915 - argininosuccinate synthase, at 901,300 to 902,538
_RS13915
BBR_RS20805: BBR_RS20805 - hypothetical protein, at 902,905 to 903,759
_RS20805
Position (kb)
901
902
903 Strain fitness (log2 ratio)
-2
-1
0
1 at 900.310 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.410 kb on + strand, within BBR_RS13905 at 900.418 kb on - strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.539 kb on + strand at 900.540 kb on + strand at 900.826 kb on + strand, within BBR_RS13910 at 900.932 kb on + strand, within BBR_RS13910 at 900.985 kb on + strand, within BBR_RS13910 at 901.159 kb on + strand at 901.167 kb on - strand at 901.171 kb on + strand at 901.179 kb on - strand at 901.190 kb on - strand at 901.205 kb on + strand at 901.213 kb on - strand at 901.316 kb on + strand at 901.326 kb on + strand at 901.329 kb on - strand at 901.339 kb on + strand at 901.339 kb on - strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.441 kb on - strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on - strand, within BBR_RS13915 at 901.489 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.567 kb on + strand, within BBR_RS13915 at 901.573 kb on - strand, within BBR_RS13915 at 901.606 kb on + strand, within BBR_RS13915 at 901.629 kb on - strand, within BBR_RS13915 at 901.676 kb on - strand, within BBR_RS13915 at 901.693 kb on + strand, within BBR_RS13915 at 901.693 kb on - strand, within BBR_RS13915 at 901.719 kb on - strand, within BBR_RS13915 at 901.728 kb on - strand, within BBR_RS13915 at 901.750 kb on + strand, within BBR_RS13915 at 901.774 kb on + strand, within BBR_RS13915 at 901.799 kb on + strand, within BBR_RS13915 at 901.809 kb on + strand, within BBR_RS13915 at 901.839 kb on + strand, within BBR_RS13915 at 901.851 kb on - strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.911 kb on + strand, within BBR_RS13915 at 901.919 kb on - strand, within BBR_RS13915 at 901.940 kb on - strand, within BBR_RS13915 at 901.998 kb on - strand, within BBR_RS13915 at 902.019 kb on - strand, within BBR_RS13915 at 902.021 kb on + strand, within BBR_RS13915 at 902.094 kb on - strand, within BBR_RS13915 at 902.105 kb on + strand, within BBR_RS13915 at 902.115 kb on + strand, within BBR_RS13915 at 902.122 kb on + strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.132 kb on + strand, within BBR_RS13915 at 902.137 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915 at 902.161 kb on + strand, within BBR_RS13915 at 902.193 kb on - strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.334 kb on - strand, within BBR_RS13915 at 902.347 kb on + strand, within BBR_RS13915 at 902.362 kb on + strand, within BBR_RS13915 at 902.362 kb on + strand, within BBR_RS13915 at 902.363 kb on + strand, within BBR_RS13915 at 902.422 kb on + strand at 902.451 kb on + strand at 902.507 kb on + strand at 902.554 kb on - strand at 902.567 kb on + strand at 902.575 kb on - strand at 902.599 kb on - strand at 902.601 kb on + strand at 902.604 kb on - strand at 902.604 kb on - strand at 902.609 kb on - strand at 902.609 kb on - strand at 902.618 kb on + strand at 902.755 kb on + strand at 902.821 kb on + strand at 902.834 kb on - strand at 902.893 kb on + strand at 902.906 kb on - strand at 902.933 kb on - strand at 902.949 kb on + strand at 903.006 kb on + strand, within BBR_RS20805 at 903.006 kb on + strand, within BBR_RS20805 at 903.014 kb on - strand, within BBR_RS20805 at 903.014 kb on - strand, within BBR_RS20805 at 903.039 kb on - strand, within BBR_RS20805 at 903.055 kb on + strand, within BBR_RS20805 at 903.084 kb on + strand, within BBR_RS20805 at 903.162 kb on - strand, within BBR_RS20805 at 903.177 kb on + strand, within BBR_RS20805 at 903.313 kb on - strand, within BBR_RS20805 at 903.326 kb on - strand, within BBR_RS20805 at 903.392 kb on + strand, within BBR_RS20805 at 903.400 kb on - strand, within BBR_RS20805
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with lactate 200 mM remove 900,310 + BBR_RS13905 0.66 +0.9 900,310 + BBR_RS13905 0.66 -0.5 900,410 + BBR_RS13905 0.77 -0.5 900,418 - BBR_RS13905 0.77 -0.3 900,425 + BBR_RS13905 0.78 -0.1 900,425 + BBR_RS13905 0.78 -0.4 900,539 + -0.1 900,540 + -0.4 900,826 + BBR_RS13910 0.38 -0.3 900,932 + BBR_RS13910 0.58 +0.1 900,985 + BBR_RS13910 0.69 -0.3 901,159 + -0.6 901,167 - -0.2 901,171 + -0.4 901,179 - +0.2 901,190 - +0.2 901,205 + -0.5 901,213 - -0.5 901,316 + +0.8 901,326 + +0.1 901,329 - -0.5 901,339 + +1.3 901,339 - -0.7 901,417 + +0.3 901,417 + -0.6 901,417 + +0.0 901,417 + -0.8 901,417 + +0.6 901,441 - BBR_RS13915 0.11 -0.8 901,442 + BBR_RS13915 0.11 -1.5 901,442 + BBR_RS13915 0.11 -0.7 901,442 - BBR_RS13915 0.11 -0.3 901,489 + BBR_RS13915 0.15 +0.4 901,539 + BBR_RS13915 0.19 +0.3 901,539 + BBR_RS13915 0.19 +0.8 901,567 + BBR_RS13915 0.22 -0.9 901,573 - BBR_RS13915 0.22 +0.0 901,606 + BBR_RS13915 0.25 +0.3 901,629 - BBR_RS13915 0.27 -0.2 901,676 - BBR_RS13915 0.30 +0.4 901,693 + BBR_RS13915 0.32 -0.3 901,693 - BBR_RS13915 0.32 +0.5 901,719 - BBR_RS13915 0.34 -0.0 901,728 - BBR_RS13915 0.35 +0.0 901,750 + BBR_RS13915 0.36 -0.0 901,774 + BBR_RS13915 0.38 -0.0 901,799 + BBR_RS13915 0.40 -1.0 901,809 + BBR_RS13915 0.41 +0.5 901,839 + BBR_RS13915 0.44 +0.9 901,851 - BBR_RS13915 0.44 -0.5 901,905 + BBR_RS13915 0.49 -0.3 901,905 + BBR_RS13915 0.49 +0.8 901,911 + BBR_RS13915 0.49 +0.9 901,919 - BBR_RS13915 0.50 -0.5 901,940 - BBR_RS13915 0.52 -0.2 901,998 - BBR_RS13915 0.56 +0.1 902,019 - BBR_RS13915 0.58 -0.4 902,021 + BBR_RS13915 0.58 +0.0 902,094 - BBR_RS13915 0.64 +0.0 902,105 + BBR_RS13915 0.65 +1.1 902,115 + BBR_RS13915 0.66 +0.3 902,122 + BBR_RS13915 0.66 +0.2 902,123 - BBR_RS13915 0.66 -0.9 902,123 - BBR_RS13915 0.66 -0.0 902,123 - BBR_RS13915 0.66 -1.0 902,123 - BBR_RS13915 0.66 -0.5 902,132 + BBR_RS13915 0.67 -0.1 902,137 + BBR_RS13915 0.68 +0.1 902,142 + BBR_RS13915 0.68 -0.6 902,142 + BBR_RS13915 0.68 -0.4 902,161 + BBR_RS13915 0.69 -0.5 902,193 - BBR_RS13915 0.72 -0.3 902,217 + BBR_RS13915 0.74 -0.5 902,217 + BBR_RS13915 0.74 +0.8 902,217 + BBR_RS13915 0.74 -0.7 902,232 + BBR_RS13915 0.75 +0.2 902,232 + BBR_RS13915 0.75 +0.8 902,232 + BBR_RS13915 0.75 +0.3 902,326 + BBR_RS13915 0.83 -0.1 902,326 + BBR_RS13915 0.83 +0.6 902,326 + BBR_RS13915 0.83 +0.1 902,326 + BBR_RS13915 0.83 -0.1 902,326 + BBR_RS13915 0.83 +0.2 902,334 - BBR_RS13915 0.83 +0.3 902,347 + BBR_RS13915 0.85 -0.2 902,362 + BBR_RS13915 0.86 -0.1 902,362 + BBR_RS13915 0.86 +0.3 902,363 + BBR_RS13915 0.86 +0.7 902,422 + -0.0 902,451 + -0.3 902,507 + +1.0 902,554 - +0.0 902,567 + +1.6 902,575 - -0.5 902,599 - +0.6 902,601 + -0.3 902,604 - -1.6 902,604 - -0.2 902,609 - +0.0 902,609 - +0.1 902,618 + +0.1 902,755 + -0.6 902,821 + +0.6 902,834 - +0.2 902,893 + -0.4 902,906 - -0.1 902,933 - +0.2 902,949 + -0.3 903,006 + BBR_RS20805 0.12 +0.3 903,006 + BBR_RS20805 0.12 +0.3 903,014 - BBR_RS20805 0.13 +0.0 903,014 - BBR_RS20805 0.13 +0.2 903,039 - BBR_RS20805 0.16 +0.9 903,055 + BBR_RS20805 0.18 -0.7 903,084 + BBR_RS20805 0.21 +0.6 903,162 - BBR_RS20805 0.30 -0.1 903,177 + BBR_RS20805 0.32 -0.1 903,313 - BBR_RS20805 0.48 +0.7 903,326 - BBR_RS20805 0.49 -0.4 903,392 + BBR_RS20805 0.57 +0.2 903,400 - BBR_RS20805 0.58 -2.7
Or see this region's nucleotide sequence