Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22985 and ABZR87_RS22990 are separated by 34 nucleotides ABZR87_RS22990 and ABZR87_RS22995 overlap by 10 nucleotides ABZR87_RS22995 and ABZR87_RS23000 overlap by 4 nucleotides
ABZR87_RS22985: ABZR87_RS22985 - glycosyltransferase family 1 protein, at 1,516,691 to 1,518,004
_RS22985
ABZR87_RS22990: ABZR87_RS22990 - SDR family oxidoreductase, at 1,518,039 to 1,518,800
_RS22990
ABZR87_RS22995: ABZR87_RS22995 - HAD-IIIA family hydrolase, at 1,518,791 to 1,519,459
_RS22995
ABZR87_RS23000: ABZR87_RS23000 - lipopolysaccharide heptosyltransferase II, at 1,519,456 to 1,520,673
_RS23000
Position (kb) 1518
1519
1520 Strain fitness (log2 ratio) -2
-1
0
1 at 1517.867 kb on + strand, within ABZR87_RS22985 at 1517.867 kb on + strand, within ABZR87_RS22985 at 1517.868 kb on - strand, within ABZR87_RS22985 at 1517.906 kb on + strand at 1518.731 kb on - strand at 1518.878 kb on + strand, within ABZR87_RS22995 at 1518.878 kb on + strand, within ABZR87_RS22995 at 1518.879 kb on - strand, within ABZR87_RS22995 at 1518.999 kb on - strand, within ABZR87_RS22995 at 1519.083 kb on - strand, within ABZR87_RS22995 at 1519.083 kb on - strand, within ABZR87_RS22995 at 1519.319 kb on + strand, within ABZR87_RS22995 at 1519.320 kb on - strand, within ABZR87_RS22995 at 1519.320 kb on - strand, within ABZR87_RS22995 at 1519.788 kb on + strand, within ABZR87_RS23000 at 1520.033 kb on + strand, within ABZR87_RS23000 at 1520.034 kb on - strand, within ABZR87_RS23000 at 1520.034 kb on - strand, within ABZR87_RS23000 at 1520.413 kb on + strand, within ABZR87_RS23000 at 1520.413 kb on + strand, within ABZR87_RS23000 at 1520.414 kb on - strand, within ABZR87_RS23000
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 1,517,867 + ABZR87_RS22985 0.89 +0.9 1,517,867 + ABZR87_RS22985 0.89 -0.0 1,517,868 - ABZR87_RS22985 0.90 -1.3 1,517,906 + +0.0 1,518,731 - +0.3 1,518,878 + ABZR87_RS22995 0.13 -1.3 1,518,878 + ABZR87_RS22995 0.13 +0.7 1,518,879 - ABZR87_RS22995 0.13 -0.3 1,518,999 - ABZR87_RS22995 0.31 -0.3 1,519,083 - ABZR87_RS22995 0.44 +0.6 1,519,083 - ABZR87_RS22995 0.44 -1.4 1,519,319 + ABZR87_RS22995 0.79 +0.8 1,519,320 - ABZR87_RS22995 0.79 -0.9 1,519,320 - ABZR87_RS22995 0.79 -0.8 1,519,788 + ABZR87_RS23000 0.27 -0.2 1,520,033 + ABZR87_RS23000 0.47 +0.8 1,520,034 - ABZR87_RS23000 0.47 -2.0 1,520,034 - ABZR87_RS23000 0.47 -1.3 1,520,413 + ABZR87_RS23000 0.79 +0.2 1,520,413 + ABZR87_RS23000 0.79 +0.3 1,520,414 - ABZR87_RS23000 0.79 +0.9
Or see this region's nucleotide sequence