Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22055 and ABZR87_RS22060 are separated by 38 nucleotides ABZR87_RS22060 and ABZR87_RS22065 are separated by 108 nucleotides ABZR87_RS22065 and ABZR87_RS22070 overlap by 11 nucleotides ABZR87_RS22070 and ABZR87_RS22075 are separated by 35 nucleotides
ABZR87_RS22055: ABZR87_RS22055 - flagellar hook-length control protein FliK, at 1,316,908 to 1,318,500
_RS22055
ABZR87_RS22060: ABZR87_RS22060 - flagellar export protein FliJ, at 1,318,539 to 1,319,003
_RS22060
ABZR87_RS22065: ABZR87_RS22065 - flagellar protein export ATPase FliI, at 1,319,112 to 1,320,548
_RS22065
ABZR87_RS22070: ABZR87_RS22070 - flagellar assembly protein FliH, at 1,320,538 to 1,321,275
_RS22070
ABZR87_RS22075: ABZR87_RS22075 - flagellar motor switch protein FliG, at 1,321,311 to 1,322,303
_RS22075
Position (kb)
1319
1320
1321 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1318.174 kb on - strand, within ABZR87_RS22055 at 1318.219 kb on - strand, within ABZR87_RS22055 at 1318.219 kb on - strand, within ABZR87_RS22055 at 1318.389 kb on + strand at 1318.390 kb on - strand at 1318.434 kb on + strand at 1318.435 kb on - strand at 1318.435 kb on - strand at 1318.789 kb on + strand, within ABZR87_RS22060 at 1318.790 kb on - strand, within ABZR87_RS22060 at 1318.850 kb on - strand, within ABZR87_RS22060 at 1318.904 kb on + strand, within ABZR87_RS22060 at 1318.904 kb on + strand, within ABZR87_RS22060 at 1319.027 kb on + strand at 1319.027 kb on + strand at 1319.027 kb on + strand at 1319.027 kb on + strand at 1319.028 kb on - strand at 1319.061 kb on + strand at 1319.248 kb on + strand at 1319.249 kb on - strand at 1319.293 kb on + strand, within ABZR87_RS22065 at 1319.293 kb on + strand, within ABZR87_RS22065 at 1319.294 kb on - strand, within ABZR87_RS22065 at 1319.294 kb on - strand, within ABZR87_RS22065 at 1319.294 kb on - strand, within ABZR87_RS22065 at 1319.500 kb on + strand, within ABZR87_RS22065 at 1319.599 kb on + strand, within ABZR87_RS22065 at 1319.713 kb on + strand, within ABZR87_RS22065 at 1319.713 kb on + strand, within ABZR87_RS22065 at 1319.713 kb on + strand, within ABZR87_RS22065 at 1319.713 kb on + strand, within ABZR87_RS22065 at 1319.713 kb on + strand, within ABZR87_RS22065 at 1319.714 kb on - strand, within ABZR87_RS22065 at 1319.734 kb on + strand, within ABZR87_RS22065 at 1319.735 kb on - strand, within ABZR87_RS22065 at 1321.232 kb on + strand at 1321.318 kb on + strand at 1321.318 kb on + strand at 1321.318 kb on + strand at 1321.319 kb on - strand at 1321.319 kb on - strand at 1321.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 1,318,174 - ABZR87_RS22055 0.79 +1.1 1,318,219 - ABZR87_RS22055 0.82 +0.7 1,318,219 - ABZR87_RS22055 0.82 +3.3 1,318,389 + +2.3 1,318,390 - +1.8 1,318,434 + +1.0 1,318,435 - +0.7 1,318,435 - +0.5 1,318,789 + ABZR87_RS22060 0.54 +1.8 1,318,790 - ABZR87_RS22060 0.54 +1.2 1,318,850 - ABZR87_RS22060 0.67 +2.9 1,318,904 + ABZR87_RS22060 0.78 +1.7 1,318,904 + ABZR87_RS22060 0.78 +2.1 1,319,027 + +0.9 1,319,027 + +0.6 1,319,027 + +1.1 1,319,027 + +0.5 1,319,028 - +0.7 1,319,061 + +1.1 1,319,248 + +2.7 1,319,249 - +1.7 1,319,293 + ABZR87_RS22065 0.13 +2.6 1,319,293 + ABZR87_RS22065 0.13 +1.6 1,319,294 - ABZR87_RS22065 0.13 +1.6 1,319,294 - ABZR87_RS22065 0.13 +2.1 1,319,294 - ABZR87_RS22065 0.13 +1.9 1,319,500 + ABZR87_RS22065 0.27 +2.1 1,319,599 + ABZR87_RS22065 0.34 +1.3 1,319,713 + ABZR87_RS22065 0.42 +2.3 1,319,713 + ABZR87_RS22065 0.42 +2.6 1,319,713 + ABZR87_RS22065 0.42 +2.6 1,319,713 + ABZR87_RS22065 0.42 +1.2 1,319,713 + ABZR87_RS22065 0.42 +2.6 1,319,714 - ABZR87_RS22065 0.42 +1.7 1,319,734 + ABZR87_RS22065 0.43 +3.0 1,319,735 - ABZR87_RS22065 0.43 +1.8 1,321,232 + +1.9 1,321,318 + +2.3 1,321,318 + +1.2 1,321,318 + +1.9 1,321,319 - +1.0 1,321,319 - +1.7 1,321,352 - +1.2
Or see this region's nucleotide sequence