Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21055 and ABZR87_RS21060 overlap by 4 nucleotides ABZR87_RS21060 and ABZR87_RS21065 overlap by 4 nucleotides ABZR87_RS21065 and ABZR87_RS21070 are separated by 28 nucleotides ABZR87_RS21070 and ABZR87_RS21075 are separated by 100 nucleotides
ABZR87_RS21055: ABZR87_RS21055 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,106,990 to 1,108,189
_RS21055
ABZR87_RS21060: ABZR87_RS21060 - DUF1365 domain-containing protein, at 1,108,186 to 1,108,953
_RS21060
ABZR87_RS21065: ABZR87_RS21065 - FAD-dependent oxidoreductase, at 1,108,950 to 1,110,245
_RS21065
ABZR87_RS21070: ABZR87_RS21070 - GTP cyclohydrolase I, at 1,110,274 to 1,111,002
_RS21070
ABZR87_RS21075: ABZR87_RS21075 - MerR family transcriptional regulator, at 1,111,103 to 1,112,089
_RS21075
Position (kb)
1108
1109
1110
1111 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1107.958 kb on - strand, within ABZR87_RS21055 at 1108.125 kb on - strand at 1108.404 kb on - strand, within ABZR87_RS21060 at 1108.414 kb on + strand, within ABZR87_RS21060 at 1108.414 kb on + strand, within ABZR87_RS21060 at 1108.556 kb on + strand, within ABZR87_RS21060 at 1108.632 kb on - strand, within ABZR87_RS21060 at 1108.735 kb on + strand, within ABZR87_RS21060 at 1108.736 kb on - strand, within ABZR87_RS21060 at 1108.736 kb on - strand, within ABZR87_RS21060 at 1108.772 kb on + strand, within ABZR87_RS21060 at 1109.039 kb on - strand at 1109.245 kb on + strand, within ABZR87_RS21065 at 1109.297 kb on - strand, within ABZR87_RS21065 at 1109.470 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.584 kb on + strand, within ABZR87_RS21065 at 1109.585 kb on - strand, within ABZR87_RS21065 at 1109.585 kb on - strand, within ABZR87_RS21065 at 1109.585 kb on - strand, within ABZR87_RS21065 at 1109.585 kb on - strand, within ABZR87_RS21065 at 1109.740 kb on + strand, within ABZR87_RS21065 at 1109.740 kb on + strand, within ABZR87_RS21065 at 1109.741 kb on - strand, within ABZR87_RS21065 at 1109.741 kb on - strand, within ABZR87_RS21065 at 1109.741 kb on - strand, within ABZR87_RS21065 at 1109.765 kb on - strand, within ABZR87_RS21065 at 1109.994 kb on + strand, within ABZR87_RS21065 at 1110.031 kb on + strand, within ABZR87_RS21065 at 1110.031 kb on + strand, within ABZR87_RS21065 at 1110.104 kb on + strand, within ABZR87_RS21065 at 1110.104 kb on + strand, within ABZR87_RS21065 at 1110.105 kb on - strand, within ABZR87_RS21065 at 1110.105 kb on - strand, within ABZR87_RS21065 at 1110.105 kb on - strand, within ABZR87_RS21065 at 1110.105 kb on - strand, within ABZR87_RS21065 at 1110.407 kb on + strand, within ABZR87_RS21070 at 1110.408 kb on - strand, within ABZR87_RS21070 at 1110.537 kb on - strand, within ABZR87_RS21070 at 1110.537 kb on - strand, within ABZR87_RS21070 at 1110.713 kb on + strand, within ABZR87_RS21070 at 1110.714 kb on - strand, within ABZR87_RS21070 at 1110.876 kb on - strand, within ABZR87_RS21070 at 1110.876 kb on - strand, within ABZR87_RS21070 at 1111.094 kb on + strand at 1111.094 kb on + strand at 1111.095 kb on - strand at 1111.095 kb on - strand at 1111.095 kb on - strand at 1111.096 kb on + strand at 1111.096 kb on + strand at 1111.096 kb on + strand at 1111.097 kb on - strand at 1111.146 kb on + strand at 1111.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 1,107,958 - ABZR87_RS21055 0.81 -1.4 1,108,125 - -3.9 1,108,404 - ABZR87_RS21060 0.28 -2.1 1,108,414 + ABZR87_RS21060 0.30 +0.1 1,108,414 + ABZR87_RS21060 0.30 -3.0 1,108,556 + ABZR87_RS21060 0.48 -2.7 1,108,632 - ABZR87_RS21060 0.58 -2.3 1,108,735 + ABZR87_RS21060 0.71 -0.7 1,108,736 - ABZR87_RS21060 0.72 -1.2 1,108,736 - ABZR87_RS21060 0.72 -3.5 1,108,772 + ABZR87_RS21060 0.76 -2.1 1,109,039 - -2.3 1,109,245 + ABZR87_RS21065 0.23 +0.5 1,109,297 - ABZR87_RS21065 0.27 -2.2 1,109,470 + ABZR87_RS21065 0.40 -3.4 1,109,584 + ABZR87_RS21065 0.49 -3.0 1,109,584 + ABZR87_RS21065 0.49 -4.3 1,109,584 + ABZR87_RS21065 0.49 -1.3 1,109,584 + ABZR87_RS21065 0.49 -1.6 1,109,584 + ABZR87_RS21065 0.49 -2.7 1,109,584 + ABZR87_RS21065 0.49 -1.2 1,109,585 - ABZR87_RS21065 0.49 -1.2 1,109,585 - ABZR87_RS21065 0.49 -3.5 1,109,585 - ABZR87_RS21065 0.49 -1.4 1,109,585 - ABZR87_RS21065 0.49 -2.4 1,109,740 + ABZR87_RS21065 0.61 -2.0 1,109,740 + ABZR87_RS21065 0.61 +0.8 1,109,741 - ABZR87_RS21065 0.61 -3.8 1,109,741 - ABZR87_RS21065 0.61 -2.3 1,109,741 - ABZR87_RS21065 0.61 -2.8 1,109,765 - ABZR87_RS21065 0.63 -0.6 1,109,994 + ABZR87_RS21065 0.81 -0.7 1,110,031 + ABZR87_RS21065 0.83 -1.7 1,110,031 + ABZR87_RS21065 0.83 -3.2 1,110,104 + ABZR87_RS21065 0.89 -2.8 1,110,104 + ABZR87_RS21065 0.89 -0.9 1,110,105 - ABZR87_RS21065 0.89 -2.3 1,110,105 - ABZR87_RS21065 0.89 -2.9 1,110,105 - ABZR87_RS21065 0.89 -2.8 1,110,105 - ABZR87_RS21065 0.89 -2.6 1,110,407 + ABZR87_RS21070 0.18 -1.3 1,110,408 - ABZR87_RS21070 0.18 -0.2 1,110,537 - ABZR87_RS21070 0.36 +0.8 1,110,537 - ABZR87_RS21070 0.36 +0.3 1,110,713 + ABZR87_RS21070 0.60 -1.0 1,110,714 - ABZR87_RS21070 0.60 +1.1 1,110,876 - ABZR87_RS21070 0.83 -0.1 1,110,876 - ABZR87_RS21070 0.83 +0.7 1,111,094 + -3.4 1,111,094 + -0.9 1,111,095 - +0.2 1,111,095 - +1.6 1,111,095 - +0.5 1,111,096 + -3.3 1,111,096 + -1.8 1,111,096 + -2.7 1,111,097 - +0.3 1,111,146 + +0.3 1,111,198 - +0.8
Or see this region's nucleotide sequence