Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS21055

Experiment: root EcoFAB sample 1; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS21045 and ABZR87_RS21050 are separated by 29 nucleotidesABZR87_RS21050 and ABZR87_RS21055 are separated by 2 nucleotidesABZR87_RS21055 and ABZR87_RS21060 overlap by 4 nucleotidesABZR87_RS21060 and ABZR87_RS21065 overlap by 4 nucleotides ABZR87_RS21045: ABZR87_RS21045 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,105,081 to 1,106,181 _RS21045 ABZR87_RS21050: ABZR87_RS21050 - DUF1295 domain-containing protein, at 1,106,211 to 1,106,987 _RS21050 ABZR87_RS21055: ABZR87_RS21055 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,106,990 to 1,108,189 _RS21055 ABZR87_RS21060: ABZR87_RS21060 - DUF1365 domain-containing protein, at 1,108,186 to 1,108,953 _RS21060 ABZR87_RS21065: ABZR87_RS21065 - FAD-dependent oxidoreductase, at 1,108,950 to 1,110,245 _RS21065 Position (kb) 1106 1107 1108 1109Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1106.200 kb on + strandat 1106.201 kb on - strandat 1106.201 kb on - strandat 1106.201 kb on - strandat 1106.209 kb on + strandat 1106.238 kb on + strandat 1106.238 kb on + strandat 1106.239 kb on - strandat 1106.257 kb on + strandat 1106.257 kb on + strandat 1106.257 kb on + strandat 1106.266 kb on + strandat 1106.267 kb on - strandat 1106.267 kb on - strandat 1106.288 kb on + strandat 1106.288 kb on + strandat 1106.398 kb on + strand, within ABZR87_RS21050at 1106.398 kb on + strand, within ABZR87_RS21050at 1106.399 kb on - strand, within ABZR87_RS21050at 1106.399 kb on - strand, within ABZR87_RS21050at 1106.428 kb on + strand, within ABZR87_RS21050at 1107.200 kb on + strand, within ABZR87_RS21055at 1107.200 kb on + strand, within ABZR87_RS21055at 1107.200 kb on + strand, within ABZR87_RS21055at 1107.201 kb on - strand, within ABZR87_RS21055at 1107.306 kb on - strand, within ABZR87_RS21055at 1107.385 kb on - strand, within ABZR87_RS21055at 1107.518 kb on - strand, within ABZR87_RS21055at 1107.757 kb on - strand, within ABZR87_RS21055at 1107.759 kb on - strand, within ABZR87_RS21055at 1107.777 kb on + strand, within ABZR87_RS21055at 1107.777 kb on + strand, within ABZR87_RS21055at 1107.778 kb on - strand, within ABZR87_RS21055at 1107.958 kb on - strand, within ABZR87_RS21055at 1108.125 kb on - strandat 1108.404 kb on - strand, within ABZR87_RS21060at 1108.414 kb on + strand, within ABZR87_RS21060at 1108.414 kb on + strand, within ABZR87_RS21060at 1108.556 kb on + strand, within ABZR87_RS21060at 1108.632 kb on - strand, within ABZR87_RS21060at 1108.735 kb on + strand, within ABZR87_RS21060at 1108.736 kb on - strand, within ABZR87_RS21060at 1108.736 kb on - strand, within ABZR87_RS21060at 1108.772 kb on + strand, within ABZR87_RS21060at 1109.039 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB
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1,106,200 + -1.4
1,106,201 - -0.5
1,106,201 - -0.5
1,106,201 - +0.4
1,106,209 + -0.7
1,106,238 + -1.3
1,106,238 + -0.4
1,106,239 - -3.7
1,106,257 + -2.0
1,106,257 + -0.8
1,106,257 + -2.0
1,106,266 + -1.5
1,106,267 - -2.1
1,106,267 - -2.9
1,106,288 + -2.5
1,106,288 + -2.7
1,106,398 + ABZR87_RS21050 0.24 -2.7
1,106,398 + ABZR87_RS21050 0.24 -0.4
1,106,399 - ABZR87_RS21050 0.24 -3.4
1,106,399 - ABZR87_RS21050 0.24 -3.1
1,106,428 + ABZR87_RS21050 0.28 -0.8
1,107,200 + ABZR87_RS21055 0.17 -2.2
1,107,200 + ABZR87_RS21055 0.17 -2.7
1,107,200 + ABZR87_RS21055 0.17 -1.8
1,107,201 - ABZR87_RS21055 0.18 -2.0
1,107,306 - ABZR87_RS21055 0.26 -4.0
1,107,385 - ABZR87_RS21055 0.33 -2.7
1,107,518 - ABZR87_RS21055 0.44 -3.7
1,107,757 - ABZR87_RS21055 0.64 -3.0
1,107,759 - ABZR87_RS21055 0.64 -0.5
1,107,777 + ABZR87_RS21055 0.66 -1.3
1,107,777 + ABZR87_RS21055 0.66 -2.0
1,107,778 - ABZR87_RS21055 0.66 -2.1
1,107,958 - ABZR87_RS21055 0.81 -1.4
1,108,125 - -3.9
1,108,404 - ABZR87_RS21060 0.28 -2.1
1,108,414 + ABZR87_RS21060 0.30 +0.1
1,108,414 + ABZR87_RS21060 0.30 -3.0
1,108,556 + ABZR87_RS21060 0.48 -2.7
1,108,632 - ABZR87_RS21060 0.58 -2.3
1,108,735 + ABZR87_RS21060 0.71 -0.7
1,108,736 - ABZR87_RS21060 0.72 -1.2
1,108,736 - ABZR87_RS21060 0.72 -3.5
1,108,772 + ABZR87_RS21060 0.76 -2.1
1,109,039 - -2.3

Or see this region's nucleotide sequence