Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS15090 and ABZR87_RS15095 are separated by 110 nucleotides ABZR87_RS15095 and ABZR87_RS15100 overlap by 4 nucleotides ABZR87_RS15100 and ABZR87_RS15105 are separated by 152 nucleotides
ABZR87_RS15090: ABZR87_RS15090 - 8-oxoguanine deaminase, at 3,184,283 to 3,185,701
_RS15090
ABZR87_RS15095: ABZR87_RS15095 - allantoinase PuuE, at 3,185,812 to 3,186,774
_RS15095
ABZR87_RS15100: ABZR87_RS15100 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, at 3,186,771 to 3,187,295
_RS15100
ABZR87_RS15105: ABZR87_RS15105 - porin, at 3,187,448 to 3,188,518
_RS15105
Position (kb)
3185
3186
3187 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3185.003 kb on + strand, within ABZR87_RS15090 at 3185.004 kb on - strand, within ABZR87_RS15090 at 3185.054 kb on + strand, within ABZR87_RS15090 at 3185.054 kb on + strand, within ABZR87_RS15090 at 3185.091 kb on - strand, within ABZR87_RS15090 at 3185.785 kb on + strand at 3185.785 kb on + strand at 3185.785 kb on + strand at 3185.786 kb on - strand at 3185.786 kb on - strand at 3185.823 kb on + strand at 3185.823 kb on + strand at 3185.823 kb on + strand at 3185.823 kb on + strand at 3185.824 kb on - strand at 3185.824 kb on - strand at 3185.824 kb on - strand at 3185.828 kb on - strand at 3185.849 kb on - strand at 3185.917 kb on + strand, within ABZR87_RS15095 at 3185.917 kb on + strand, within ABZR87_RS15095 at 3185.918 kb on - strand, within ABZR87_RS15095 at 3186.015 kb on + strand, within ABZR87_RS15095 at 3186.016 kb on - strand, within ABZR87_RS15095 at 3186.016 kb on - strand, within ABZR87_RS15095 at 3186.034 kb on + strand, within ABZR87_RS15095 at 3186.034 kb on + strand, within ABZR87_RS15095 at 3186.034 kb on + strand, within ABZR87_RS15095 at 3186.035 kb on - strand, within ABZR87_RS15095 at 3186.035 kb on - strand, within ABZR87_RS15095 at 3186.035 kb on - strand, within ABZR87_RS15095 at 3186.035 kb on - strand, within ABZR87_RS15095 at 3186.035 kb on - strand, within ABZR87_RS15095 at 3186.059 kb on + strand, within ABZR87_RS15095 at 3186.208 kb on + strand, within ABZR87_RS15095 at 3186.301 kb on + strand, within ABZR87_RS15095 at 3186.301 kb on + strand, within ABZR87_RS15095 at 3186.373 kb on + strand, within ABZR87_RS15095 at 3186.374 kb on - strand, within ABZR87_RS15095 at 3186.374 kb on - strand, within ABZR87_RS15095 at 3186.376 kb on + strand, within ABZR87_RS15095 at 3186.376 kb on + strand, within ABZR87_RS15095 at 3186.452 kb on - strand, within ABZR87_RS15095 at 3186.452 kb on - strand, within ABZR87_RS15095 at 3186.452 kb on - strand, within ABZR87_RS15095 at 3186.517 kb on + strand, within ABZR87_RS15095 at 3186.517 kb on + strand, within ABZR87_RS15095 at 3186.517 kb on + strand, within ABZR87_RS15095 at 3186.518 kb on - strand, within ABZR87_RS15095 at 3186.523 kb on + strand, within ABZR87_RS15095 at 3186.524 kb on - strand, within ABZR87_RS15095 at 3186.550 kb on + strand, within ABZR87_RS15095 at 3186.551 kb on - strand, within ABZR87_RS15095 at 3186.665 kb on - strand, within ABZR87_RS15095 at 3186.739 kb on + strand at 3186.739 kb on + strand at 3186.739 kb on + strand at 3186.787 kb on - strand at 3186.787 kb on - strand at 3186.787 kb on - strand at 3186.849 kb on + strand, within ABZR87_RS15100 at 3186.849 kb on + strand, within ABZR87_RS15100 at 3186.850 kb on - strand, within ABZR87_RS15100 at 3186.850 kb on - strand, within ABZR87_RS15100 at 3187.116 kb on + strand, within ABZR87_RS15100 at 3187.116 kb on + strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.117 kb on - strand, within ABZR87_RS15100 at 3187.162 kb on - strand, within ABZR87_RS15100 at 3187.443 kb on + strand at 3187.443 kb on + strand at 3187.443 kb on + strand at 3187.443 kb on + strand at 3187.444 kb on - strand at 3187.444 kb on - strand at 3187.444 kb on - strand at 3187.548 kb on - strand at 3187.548 kb on - strand at 3187.548 kb on - strand at 3187.640 kb on + strand, within ABZR87_RS15105 at 3187.640 kb on + strand, within ABZR87_RS15105 at 3187.731 kb on - strand, within ABZR87_RS15105
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 3,185,003 + ABZR87_RS15090 0.51 -1.6 3,185,004 - ABZR87_RS15090 0.51 +0.2 3,185,054 + ABZR87_RS15090 0.54 -0.6 3,185,054 + ABZR87_RS15090 0.54 +0.6 3,185,091 - ABZR87_RS15090 0.57 +0.5 3,185,785 + -0.2 3,185,785 + +0.3 3,185,785 + +0.8 3,185,786 - -0.1 3,185,786 - +0.2 3,185,823 + -1.1 3,185,823 + -0.1 3,185,823 + -1.7 3,185,823 + -0.8 3,185,824 - -0.1 3,185,824 - -1.9 3,185,824 - +1.7 3,185,828 - -1.6 3,185,849 - -0.2 3,185,917 + ABZR87_RS15095 0.11 -1.3 3,185,917 + ABZR87_RS15095 0.11 -0.4 3,185,918 - ABZR87_RS15095 0.11 -0.1 3,186,015 + ABZR87_RS15095 0.21 -3.2 3,186,016 - ABZR87_RS15095 0.21 -3.4 3,186,016 - ABZR87_RS15095 0.21 +0.2 3,186,034 + ABZR87_RS15095 0.23 -3.2 3,186,034 + ABZR87_RS15095 0.23 -0.4 3,186,034 + ABZR87_RS15095 0.23 -2.0 3,186,035 - ABZR87_RS15095 0.23 -0.5 3,186,035 - ABZR87_RS15095 0.23 -1.5 3,186,035 - ABZR87_RS15095 0.23 +2.4 3,186,035 - ABZR87_RS15095 0.23 -1.4 3,186,035 - ABZR87_RS15095 0.23 +0.2 3,186,059 + ABZR87_RS15095 0.26 -1.5 3,186,208 + ABZR87_RS15095 0.41 +0.2 3,186,301 + ABZR87_RS15095 0.51 -3.0 3,186,301 + ABZR87_RS15095 0.51 -2.1 3,186,373 + ABZR87_RS15095 0.58 -3.1 3,186,374 - ABZR87_RS15095 0.58 +1.4 3,186,374 - ABZR87_RS15095 0.58 -1.8 3,186,376 + ABZR87_RS15095 0.59 -2.6 3,186,376 + ABZR87_RS15095 0.59 -2.5 3,186,452 - ABZR87_RS15095 0.66 -2.8 3,186,452 - ABZR87_RS15095 0.66 -0.1 3,186,452 - ABZR87_RS15095 0.66 -0.8 3,186,517 + ABZR87_RS15095 0.73 +0.7 3,186,517 + ABZR87_RS15095 0.73 -0.8 3,186,517 + ABZR87_RS15095 0.73 -0.7 3,186,518 - ABZR87_RS15095 0.73 -1.5 3,186,523 + ABZR87_RS15095 0.74 -0.5 3,186,524 - ABZR87_RS15095 0.74 -0.7 3,186,550 + ABZR87_RS15095 0.77 -1.0 3,186,551 - ABZR87_RS15095 0.77 -2.4 3,186,665 - ABZR87_RS15095 0.89 +1.8 3,186,739 + +0.1 3,186,739 + +0.5 3,186,739 + +0.5 3,186,787 - -1.0 3,186,787 - +1.2 3,186,787 - +2.1 3,186,849 + ABZR87_RS15100 0.15 -1.0 3,186,849 + ABZR87_RS15100 0.15 +0.1 3,186,850 - ABZR87_RS15100 0.15 -0.3 3,186,850 - ABZR87_RS15100 0.15 -0.5 3,187,116 + ABZR87_RS15100 0.66 +1.5 3,187,116 + ABZR87_RS15100 0.66 -0.3 3,187,117 - ABZR87_RS15100 0.66 -1.3 3,187,117 - ABZR87_RS15100 0.66 +0.8 3,187,117 - ABZR87_RS15100 0.66 -0.7 3,187,117 - ABZR87_RS15100 0.66 +0.1 3,187,117 - ABZR87_RS15100 0.66 -0.2 3,187,117 - ABZR87_RS15100 0.66 +0.1 3,187,162 - ABZR87_RS15100 0.74 +0.9 3,187,443 + -0.4 3,187,443 + +0.9 3,187,443 + +0.9 3,187,443 + +1.2 3,187,444 - +2.6 3,187,444 - -0.7 3,187,444 - +1.2 3,187,548 - -0.1 3,187,548 - +0.1 3,187,548 - +0.7 3,187,640 + ABZR87_RS15105 0.18 +0.9 3,187,640 + ABZR87_RS15105 0.18 -0.0 3,187,731 - ABZR87_RS15105 0.26 -0.6
Or see this region's nucleotide sequence